trmD
tRNA (guanine-N(1)-)-methyltransferaseBBF10K_001773
source
Mesoplasma florum L1
methylates guanosine-37 in various tRNAs; usesS-adenosyl-L-methionine to transfer methyl group to tRNA
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-trmD
plasmid size
2795
insert size
732
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE