9N7polA
9°N-7 DNA polymeraseBBF10K_003247
source
Thermococcus sp. 9°N-7
thermophilic DNA polymerase; genetically engineered to have a decreased 3'→ 5' proofreading exonuclease activity (1-5% of the wildtype). Features a half-life of 6.7 hours at 95°C.
attr.
Chiara Gandini, Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-9N7polA
plasmid size
4451
insert size
2328
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE