KODpol
KOD DNA polymeraseBBF10K_003252
source
Thermococcus kodakarensis (Pyrococcus sp. KOD1)
High fidelity DNA polymerase designed for accurate PCR amplification of long strand and GC- rich DNA templates for cloning and cDNA amplification applications. Generates blunt-ended PCR product. Lacks 3'-5' exonuclease activity
attr.
Chiara Gandini, Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-KODpol
plasmid size
4451
insert size
2328
origin
ColE1 High Copy
copy number
high (3-500)
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE