Pwopol

Pwo DNA Polymerase
BBF10K_003254

source

Pyrococcus woesei

DNA polymerase; additionally exhibits 3' to 5' exonuclease activity.

attr.

Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain

{shipping_strain}

growth conditions

37 C, shaking 300 rpm

antibiotic

ampicillin

expression

strain

N/A

promoter

N/A

inducer

N/A

cloning

method

GoldenGate

enzyme

BsaI

overhangs

3' - AATG … GCTT - 5'

sequencing

forward primer

M13 For

reverse primer

M13 Rev

Construct

plasmid name

pOpen-Pwopol

plasmid size

4451

insert size

2328

origin

ColE1 High Copy

copy number

high (3-500)

genbank file

Safety

BSL

BSL1

other information

No Value

References

primary references

NCBI

No Value

UniProt

P61876

Available Elsewhere

FALSE

protocols and instructions

https://docs.google.com/document/d/1c8PyT1Z9cWYUif4XQzcWl8MPbqVuL8-RNKCAancaVQU/edit, https://openbioeconomy.org/projects/expression-guide/

citations

Frey, B. and Suppmann, B., 1995. Demonstration of the Expand PCR system’s greater fidelity and higher yields with a lacI-based PCR fidelity assay. Biochemica, 2, pp.8-9. Ghasemi, A., Salmanian, A.H., Sadeghifard, N., Salarian, A.A. and Gholi, M.K., 2011. Cloning, expression and purification of Pwo polymerase from Pyrococcus woesei. Iranian journal of microbiology, 3(3), p.118.

intellectual property

We are unaware of third-party property rights claims on uses of this item.