GBDpol
Pyrococcus Sp. Heat-Stable DNA Polymerase (Deep Vent™ at NEB)BBF10K_003256
source
Pyrococcus sp. GB-D
Robust and extremely thermostable polymerase with a half-life of 23 hours at 95°C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences.
attr.
Chiara Gandini, Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-GBDpol
plasmid size
4439
insert size
2316
origin
ColE1 High Copy
copy number
high (3-500)
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE