THEAQpolA
Taq DNA PolymeraseBBF10K_003257
source
Thermus aquaticus
DNA polymerase used in (routine) PCR method because of its high thermostability, standard testing, screening and output testing. Has a relatively high error rate because it lacks 3'-5' exonuclease activity and proofreading functionality. 5'→3' flap endonuclease degrades displaced strand."
attr.
Chiara Gandini, Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-THEAQpolA
plasmid size
4622
insert size
2499
origin
ColE1 High Copy
copy number
high (3-500)
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE