TthpolA
Tth DNA polymeraseBBF10K_003260
source
Thermus thermophilus
DNA Polymerase whose activity is resistant to prolonged incubations at high temperatures (95 °C); can therefore be used for PCR amplification of DNA fragments. Also accepts modified deoxyribonucleotides as substrates and can thus be used to label DNA fragments with radiolabeled nucleotides, digoxigenin, or biotin. Intrinsic Mn-dependent RT activity allows the enzyme to efficiently transcribe RNA targets into cDNA. Tth DNA Polymerase has been used for real time PCR.
attr.
Chiara Gandini, Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-TthpolA
plasmid size
4628
insert size
2505
origin
ColE1 High Copy
copy number
high (3-500)
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE