Bstpol
Bst DNA Polymerase, Full LengthBBF10K_003261
source
Bacillus stearothermophilus
full length DNA polymerase from Bacillus stearothermophilus. Has 5' → 3' polymerase and double-strand specific 5' → 3' exonuclease activity, but lacks the 3' → 5' exonuclease activity. Used for isothermal DNA amplification, primer extension, etc.
attr.
Chiara Gandini, Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-Bstpol
plasmid size
4754
insert size
2631
origin
ColE1 High Copy
copy number
high (3-500)
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE