T5gene122
T5 DNA PolymeraseBBF10K_003269
source
Escherichia phage T5 (Bacteriophage T5)
Replicates the viral genomic DNA. Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'-5' direction for proofreading purposes. The DNA synthesis very likely occurs by strand displacement.
attr.
Chiara Gandini, Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-T5gene122
plasmid size
4691
insert size
2568
origin
ColE1 High Copy
copy number
high (3-500)
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE