EcoRIM
EcoRI methyltransferaseBBF10K_003282
source
Escherichia coli (strain?)
Methylase that recognizes the double-stranded sequence GAATTC, causes specific methylation on A-3 on both strands, and protects the DNA from cleavage by the EcoRI endonuclease.
attr.
Chiara Gandini, Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-EcoRIM
plasmid size
3104
insert size
981
origin
ColE1 High Copy
copy number
high (3-500)
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE