TthRT

Thermus thermophilus (Tth) RT
BBF10K_003287

source

Thermus thermophilus

DNA polymerase that efficiently reverse-transcribes RNA in the presence of MnCl2

attr.

Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain

{shipping_strain}

growth conditions

37 C, shaking 300 rpm

antibiotic

ampicillin

expression

strain

N/A

promoter

N/A

inducer

N/A

cloning

method

GoldenGate

enzyme

BsaI

overhangs

3' - AATG … GCTT - 5'

sequencing

forward primer

M13 For

reverse primer

M13 Rev

Construct

plasmid name

pOpen-TthRT

plasmid size

4628

insert size

2505

origin

ColE1 High Copy

copy number

high (3-500)

genbank file

Safety

BSL

BSL1

other information

No Value

References

primary references

NCBI

No Value

UniProt

No Value

Available Elsewhere

FALSE

protocols and instructions

https://docs.google.com/document/d/1c8PyT1Z9cWYUif4XQzcWl8MPbqVuL8-RNKCAancaVQU/edit, https://openbioeconomy.org/projects/expression-guide/

citations

RÜTTIMANN, C., COTORÁS, M., ZALDÍÅ, J. and VICUÑA, R., 1985. DNA polymerases from the extremely thermophilic bacterium Thermus thermophilus HB‐8. European journal of biochemistry, 149(1), pp.41-46.https://doi.org/10.1111/j.1432-1033.1985.tb08890.x Myers, T.W. and Gelfand, D.H., 1991. Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase. Biochemistry, 30(31), pp.7661-7666. DOI: 10.1021/bi00245a001 Auer, T., Landre, P.A. and Myers, T.W., 1995. Properties of the 5' - 3'Exonuclease/Ribonuclease H Activity of Thermus thermophilus DNA Polymerase. Biochemistry, 34(15), pp.4994-5002. DOI: 10.1021/bi00015a010

intellectual property

We are unaware of third-party property rights claims on uses of this item.