SP6RNAp
SP6 RNA PolymeraseBBF10K_003291
source
Enterobacteria phage SP6 (bacteriophage SP6
DNA-dependent RNA polymerase derived from SP6 bacteriophage that catalyzes the transcription of viral genes.
attr.
Chiara Gandini, Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-SP6rpol
plasmid size
4748
insert size
2625
origin
ColE1 High Copy
copy number
high (3-500)
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE