T7EI
T7EI (T7 Endonuclease I)BBF10K_003319
source
Escherichia phage T7 (Bacteriophage T7)
DNA Endonuclease; catalyzes the cleavage of DNA mismatches and non-β DNA structures including Holliday junctions and cruciform leaving 3'-OH and 5'-phosphate. best at C mismatches and does not recognize all DNA mismatches. To a lesser extent cleaves across a nick in dsDNA; Key enzyme for use in genome editing mutation detection workflows
attr.
Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-T7EI
plasmid size
2573
insert size
471
origin
ColE1
copy number
high (3-500)
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE