CE_beta-galactosidase
CE_beta-galactosidaseBBF10K_003366
source
No Value
colorimetric reporter; hydrolyse β-galactosides into monosaccharides through the breaking of a glycosidic bond. cleaving of chromogenic 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside(X-gal) or chlorophenol red (CPR)–β-D-galactopyranoside produce a change of colour
attr.
Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-beta-galactosidase
plasmid size
5192
insert size
3069
origin
ColE1
copy number
high (3-500)
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE