beta-lactamase
beta-lactamaseBBF10K_003371
source
No Value
Cleaves a wide range of beta-lactam compounds (usually antibiotics) but also efficiently cleaves chromogenic substrates such as PADAC and nitrocefin resulting useful as reporter gene
attr.
Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-beta-lactamase
plasmid size
2984
insert size
861
origin
ColE1
copy number
high (3-500)
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE