BT1_BCD2
BT1_BCD2BBF10K_003384
source
N/A
A strong BiCistronic Designed (BCDs) RBS. BCDs reduce the variation in RBS-CDS pairing. Defined as a BN1 part.
attr.
Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - GGAG … TACT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-BT1_BCD2
plasmid size
2413
insert size
93
origin
ColE1
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE