BT1_BCD2_His
BT1_BCD2_HisBBF10K_003389
source
N/A
A strong BiCistronic Designed (BCDs) RBS. Additionally contains a his tag. BCDs reduce the variation in RBS-CDS pairing. Defined as a BN1 part.
attr.
Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - TACT … CCAT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-BT1_BCD2_His
plasmid size
2443
insert size
123
origin
ColE1
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE