BT1_RiboJ_B34_His
BT1_RiboJ_B34_HisBBF10K_003390
source
N/A
Riboj is a ribozyme that reduces DNA context from expression. Additionally contains His tag. Defined as a BN1 part.
attr.
Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - TACT … CCAT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-BT1_RiboJ_B34_His
plasmid size
2457
insert size
137
origin
ColE1
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE