T1T2_H6
T1T2_H6BBF10K_003394
source
N/A
A 6his N terminal purification tag. Defined as a N1N2 part. Has not been successfully synthesized.
attr.
Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - TACT … CCAT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-T1T2_H6
plasmid size
2409
insert size
31
origin
ColE1
copy number
500-700
Safety
BSL
BSL1
other information
No ValueReferences
Available Elsewhere
FALSE