BC_BCD2_His_TEVcut
BC_BCD2_His_TEVcutBBF10K_003419
source
N/A
A BCD combined with a his N terminal purification tag and a TEV cleavage site. Defined as a BC part.
attr.
Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - GTCA … AATG - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-BC_BCD2_His_TEVcut
plasmid size
2434
insert size
154
origin
ColE1
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE