T2T3_int_∆I
T2T3_int_∆IBBF10K_003421
source
No Value
A mini intein based on the Mtu RecA intein. Defined as a T2T3 part.
attr.
Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - TACT … AATG - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-T2T3_int_∆I
plasmid size
2640
insert size
520
origin
ColE1
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE