T2T3_int_∆I

T2T3_int_∆I
BBF10K_003421
source
No Value

A mini intein based on the Mtu RecA intein. Defined as a T2T3 part.

attr.
Open Bioeconomy Lab

Usage

growth

shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - TACT … AATG - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-T2T3_int_∆I
plasmid size
2640
insert size
520
origin
ColE1
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.