CD_EcRNAseH
CD_EcRNAseHBBF10K_003450
source
No Value
Thermostable RNAseH, 5-fold more active than Tth at 37C, degrades DNA:RNA hybrids after reverse transcription. Defined as a CD part.
attr.
Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … AGGT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-CD_EcRNAseH
plasmid size
2588
insert size
468
origin
ColE1
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE