DT4_TEV
DT4_TEVBBF10K_003453
source
N/A
TEV protease recognition linker, useful for protein purification. Defined as a DT4 part.
attr.
Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AGGT … TTCG - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-DT4_TEV
plasmid size
2409
insert size
39
origin
ColE1
copy number
500-700
Safety
BSL
BSL1
other information
No ValueReferences
Available Elsewhere
FALSE