DF_TEVcut_His_Stop_TZ
DF_TEVcut_His_Stop_TZBBF10K_003459
source
N/A
A TEV protease recognition domain, HIS tag, and stop codon. Defined as a DF part.
attr.
Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AGGT … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-DF_TEVcut_His_Stop_TZ
plasmid size
2495
insert size
375
origin
ColE1
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE