T4T5_Int_SaltS
T4T5_Int_SaltSBBF10K_003462
source
N/A
Salt-cleavable intein. Cleaves itself in the presence of 1.5 M NaCl (has little to no activity at 1 M NaCl). Usable as self-cleaving c-terminal tag. Defined as a T4T5 part.
attr.
Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - TTCG … CGGC - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-T4T5_Int_SaltS
plasmid size
2735
insert size
615
origin
ColE1
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE