T5E_6H
T5E_6HBBF10K_003467
source
N/A
A 6his C terminal purification tag. Defined as a T5E part.
attr.
Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - TTCG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-T5E_6H
plasmid size
2417
insert size
31
origin
ColE1
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE