HIV-RT
His-tagged HIV-RT p66BBF10K_003494
source
Human immunodeficiency virus 1 (HIV-1)
Human Immunodeficiency Virus reverse transcriptase expression plasmid. Apparently expressed better than most other RTs in E.coli. Originally created by the Dickinson Lab at the University of Chicago
attr.
Dickinson lab, UC Chicago
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Kanamycin
expression
strain
Escherichia coli
promoter
T7
inducer
LacI
cloning
method
N/A
enzyme
N/A
overhangs
3' - N/A … N/A - 5'
sequencing
forward primer
N/A
reverse primer
N/A
Construct
plasmid name
pET30A-HIV-RT
plasmid size
6930
insert size
1686
origin
ColE1
copy number
high (3-500)
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE