pBuild

pOpen_v3
BBF10K_003498

source

Synthetic

Robust, flexible E. coli cloning vector. Designed to be compatible with multiple cloning strategies including restriction ligation, Gibson assembly, SLiCE, and Golden Gate assembly using the FreeGenes MoClo standard.

attr.

Keoni Gandall

Usage

growth

shipping strain

{shipping_strain}

growth conditions

37 C, shaking 300 rpm

antibiotic

Ampicillin

expression

strain

Escherichia coli

promoter

N/A

inducer

N/A

cloning

method

GoldenGate

enzyme

BbsI, BtgZI, AarI

overhangs

3' - varies … varies - 5'

sequencing

forward primer

M13 For

reverse primer

M13 Rev

Construct

plasmid name

pOpen_build

plasmid size

2687

insert size

690

origin

ColE1

copy number

high (3-500)

genbank file

Safety

BSL

BSL1

other information

No Value

References

primary references

NCBI

N/A

UniProt

N/A

Available Elsewhere

FALSE

protocols and instructions

https://docs.google.com/document/d/1c8PyT1Z9cWYUif4XQzcWl8MPbqVuL8-RNKCAancaVQU/edit

citations

N/A

intellectual property

We are unaware of third-party property rights claims on uses of this item.