EcPPA Pyrophosphatase
Inorganic pyrophosphatase from E.coliBBF10K_003504
source
Escherichia coli
Used in NASBA test. Used for generation of phosphate for in-vitro reactions. This is an expression vector, inducible with IPTG and his-tagged
attr.
Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin
expression
strain
Escherichia coli
promoter
T7
inducer
LacIq
cloning
method
N/A
enzyme
N/A
overhangs
3' - N/A … N/A - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-EcPPA Pyrophosphatase
plasmid size
4404
insert size
2403
origin
ColE1
copy number
high (3-500)
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE