LOCUS       Exported     3410 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3410
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1423..1470
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3298..3314
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3315..3346
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0001"
                     /note="Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids."
                     /translation="MNVNDILKELKLSLMANKNIDESVYNDYIKTINIHKKGFSDYIVVVKSQFGLLAIKQFRQTIENEIKNILKEPVNISFTYEQEYKKQLEKDELINKDHSDIITKKVKKTNENTFENFVIGASNEQAFIAVQTVSKNPGISYNPLFIYGESGMGKTHLLKAAKNYIESNFSDLKVSYMSGDEFARKAVDILQKTHKEIEQFKNEVCQNDVLIIDDVQFLSYKEKTNEIFFTIFNNFIENDKQLFFSSDKSPELLNGFDNRLITRFNMGLSIAIQKLDNKTATAIIKKEIKNQNIKSEVTSEAINFISNYYSDDVRKIKGSVSRLNFWSQQNPEEKIITIEIISDLFRDIPTSKLGILNVKKIKEVVSEKYGISVNAIDGKARSKSIVTARHIAMFLTKEILNHTLAQIGEEFGGRDHTTVINAERKIETMLKKDKQLKKTVDILKNKILTK"
     primer_bind     complement(1475..1491)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1558..2146)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2317..3177)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3178..3282)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0001"
                     /note="Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids."
                     /translation="MNVNDILKELKLSLMANKNIDESVYNDYIKTINIHKKGFSDYIVVVKSQFGLLAIKQFRQTIENEIKNILKEPVNISFTYEQEYKKQLEKDELINKDHSDIITKKVKKTNENTFENFVIGASNEQAFIAVQTVSKNPGISYNPLFIYGESGMGKTHLLKAAKNYIESNFSDLKVSYMSGDEFARKAVDILQKTHKEIEQFKNEVCQNDVLIIDDVQFLSYKEKTNEIFFTIFNNFIENDKQLFFSSDKSPELLNGFDNRLITRFNMGLSIAIQKLDNKTATAIIKKEIKNQNIKSEVTSEAINFISNYYSDDVRKIKGSVSRLNFWSQQNPEEKIITIEIISDLFRDIPTSKLGILNVKKIKEVVSEKYGISVNAIDGKARSKSIVTARHIAMFLTKEILNHTLAQIGEEFGGRDHTTVINAERKIETMLKKDKQLKKTVDILKNKILTK"
ORIGIN
        1 ATGAACGTAA ATGACATTCT GAAGGAATTG AAACTTTCTC TGATGGCAAA TAAGAACATC
       61 GACGAGAGTG TCTATAACGA CTATATCAAG ACAATTAACA TTCACAAGAA GGGTTTTTCG
      121 GATTATATTG TAGTAGTTAA GAGCCAATTC GGATTGCTTG CCATTAAACA ATTTCGTCAG
      181 ACTATCGAAA ATGAGATTAA GAATATTTTA AAAGAGCCAG TGAATATCAG CTTCACTTAC
      241 GAGCAGGAGT ATAAGAAACA ACTGGAAAAA GACGAATTAA TTAATAAAGA TCACTCGGAC
      301 ATCATTACTA AGAAAGTGAA AAAGACTAAC GAAAATACTT TTGAAAACTT CGTGATTGGC
      361 GCAAGTAACG AACAGGCATT TATTGCCGTT CAGACTGTTT CCAAAAATCC AGGTATCAGT
      421 TATAATCCTC TTTTCATTTA CGGTGAGTCA GGTATGGGTA AAACCCATTT GTTAAAGGCT
      481 GCTAAGAACT ATATCGAGTC AAATTTTTCT GACTTGAAAG TTTCTTACAT GAGTGGTGAT
      541 GAGTTCGCAC GTAAGGCCGT GGATATCCTT CAGAAAACTC ACAAGGAGAT TGAACAATTT
      601 AAGAACGAAG TATGTCAAAA TGATGTTCTG ATTATTGATG ACGTACAGTT CTTGTCATAC
      661 AAGGAAAAGA CGAATGAAAT CTTTTTCACC ATCTTTAATA ACTTCATCGA GAATGATAAA
      721 CAGCTTTTCT TCTCTTCCGA CAAGTCCCCA GAGCTTTTGA ACGGATTTGA TAATCGCTTG
      781 ATCACGCGCT TTAACATGGG CCTGTCTATC GCCATTCAAA AATTAGACAA CAAAACTGCT
      841 ACGGCGATCA TTAAAAAGGA GATCAAGAAC CAAAATATCA AAAGCGAAGT CACTTCGGAG
      901 GCAATCAACT TCATTAGTAA CTATTACTCT GATGATGTTC GCAAAATCAA GGGCTCTGTC
      961 TCACGCTTGA ATTTTTGGTC ACAGCAGAAC CCGGAAGAGA AGATTATCAC CATCGAAATC
     1021 ATCTCAGACC TTTTCCGTGA TATTCCTACG AGCAAACTTG GAATTTTAAA CGTTAAGAAA
     1081 ATTAAAGAGG TAGTCTCCGA AAAGTATGGT ATCTCAGTGA ACGCTATCGA TGGGAAGGCC
     1141 CGCAGCAAGT CCATTGTCAC TGCGCGCCAT ATCGCAATGT TCTTGACGAA GGAAATTCTT
     1201 AATCACACCC TGGCACAAAT TGGAGAGGAG TTCGGTGGAC GCGATCATAC TACAGTCATC
     1261 AACGCTGAAC GTAAGATTGA AACGATGCTG AAAAAAGACA AGCAATTGAA AAAGACGGTT
     1321 GATATCTTAA AGAATAAAAT TTTAACTAAG TGAAGAGCTT AGAGACCGGT ACTAGTTCCT
     1381 GCAGGGCTCG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA ACCCCTTGGG
     1441 GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT GAGAGCTTGG
     1501 CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG CCATTAATTT
     1561 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
     1621 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
     1681 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
     1741 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
     1801 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
     1861 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
     1921 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
     1981 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
     2041 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
     2101 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
     2161 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT
     2221 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC
     2281 AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC
     2341 ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA
     2401 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA
     2461 ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG
     2521 CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC
     2581 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT
     2641 CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG
     2701 GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT
     2761 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA
     2821 TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA
     2881 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA
     2941 TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG
     3001 GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC
     3061 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG
     3121 AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT
     3181 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT
     3241 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA
     3301 AAACGACGGC CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT CAGGTGGAGT
     3361 GAATTCGCGG CCGCTTCTAG AGGCTCTTCG TAATTACGAA GAGGTCTCTA
//