LOCUS       BBF10K_000004           3485 bp ds-DNA     circular     17-MAY-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator      1..3485
                     /label="""transcription terminator for bacteriophage T7 RNA"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     source          1..3485
                     /label="source"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      38..85
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      complement(173..761)
                     /label="ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(952..1812)
                     /label="bla"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     exon            complement(952..1812)
                     /label="Translation 1812-952"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     1933..1949
                     /label="M13 for"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      1950..1981
                     /label="tonB terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2002..3485
                     /label="MERS negative control"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 cgctgaggtg tcaatcgtcg gagccgctga gcaataacta gcataacccc ttggggcctc
       61 taaacgggtc ttgaggggtt ttttgcatgg tcatagctgt ttcctgagag cttggcaggt
      121 gatgacacac attaacaaat ttcgtgagga gtctccagaa gaatgccatt aatttccata
      181 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      241 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      301 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      361 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      421 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      481 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      541 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
      601 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
      661 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
      721 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaaggccta ctattagcaa
      781 caacgatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
      841 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
      901 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
      961 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1021 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1081 aatgataccg cgagaaccac gctcaccggc tccagattta tcagcaataa accagccagc
     1141 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     1201 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     1261 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     1321 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     1381 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     1441 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     1501 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     1561 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     1621 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     1681 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     1741 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     1801 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     1861 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     1921 ctgcaccagt cagtaaaacg acggccagta gtcaaaagcc tccgaccgga ggcttttgac
     1981 ttggttcagg tggagtggga gCGTCACTAG AGGGCCCTGC ATTAGGATCG ATCTCGATCC
     2041 CGCGAAATTA ATACGACTCA CTATAGGGGC AACGCGATGA CGATGGATAG CGATTCATCG
     2101 ATGAGCTGAC CCGATCGCCG CCGCCGGAGG GTTGCGTTTG AGACGGGCGA CAGATATGGC
     2161 ATCCCCTGCT GCACCTCGTG CTGTTTCCTT TGCCGATAAC AATGATATAA CAAATACAAA
     2221 CCTATCTCGA GGTAGAGGAC GTAATCCAAA ACCACGAGCT GCACCAAATA ACACTGTCTC
     2281 TTGGTACACT GGGCTTACCC AACACGGGAA AGTCCCTCTT ACCTTTCCAC CTGGGCAGGG
     2341 TGTACCTCTT AATGCCAATT CTACCCCTGC GCAAAATGCT GGGTATTGGC GGAGACAGGA
     2401 CAGAAAAATT AATACCGGGA ATGGAATTAA GCAACTGGCT CCCAGGTGGT ACTTCTACTA
     2461 CACTGGAACT GGACCCGAAG CAGCACTCCC ATTCCGGGCT GTTAAGGATG GCATCGTTTG
     2521 GGTCCATGAA GATGGCGCCA CTGATGCTCC TTCAACTTTT GGGACGCGGA ACCCTAACAA
     2581 TGATTCAGCT ATTGTTACAC AATTCGCGCC CGGTACTAAG CTTCCTAAAA ACTTCCACAT
     2641 TGAGGGGACT GGAGGCAATA GTCAATCATC TTCAAGAGCC TCTAGCTTAA GCAGAAACTC
     2701 TTCCAGATCT AGTTCACAAG GTTCAAGATC AGGAAACTCT ACCCGCGGCA CTTCTCCAGG
     2761 TCCATCTGGA ATCGGAGCAG TAGGAGGTGA TCTACTTTAC CTTGATCTTC TGAACAGACT
     2821 ACAAGCCCTT GAGTCTGGCA AAGTAAAGCA ATCGCAGCCA AAAGTAATCA CTAAGAAAGA
     2881 TGCTGCTGCT GCTAAAAATA AGATGCGCCA CAAGCGCACT TCCACCAAAA GTTTCAACAT
     2941 GGTGCAAGCT TTTGGTCTTC GCGGACCAGG AGACCTCCAG GGAAACTTTG GTGATCTTCA
     3001 ATTGAATAAA CTCGGCACTG AGGACCCACG TTGGCCCCAA ATTGCTGAGC TTGCTCCTAC
     3061 AGCCAGTGCT TTTATGGGTA TGTCGCAATT TAAACTTACC CATCAGAACA ATGATGATCA
     3121 TGGCAACCCT GTGTACTTCC TTCGGTACAG TGGAGCCATT AAACTTGACC CAAAGAATCC
     3181 CAACTACAAT AAGTGGTTGG AGCTTCTTGA GCAAAATATT GATGCCTACA AAACCTTCCC
     3241 TAAGAAGGAA AAGAAACAAA AGGCACCAAA AGAAGAATCA ACAGACCAAA TGTCTGAACC
     3301 TCCAAAGGAG CAGCGTGTGC AAGGTAGCAT CACTCAGCGC ACTCGCACCC GTCCAAGTGT
     3361 TCAGCCTGGT CCAATGATTG ATGTTAACAC TGATTAGGCT TCGCCTAGGC CGCTGAGCAA
     3421 TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAACC
     3481 TCGCT
//