LOCUS       BBF10K_000006           8846 bp ds-DNA     circular     17-MAY-2022
DEFINITION  .
KEYWORDS    "marker:KanR"                                                      
FEATURES             Location/Qualifiers
     source          1..8846
                     /label="source"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      38..85
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    265..515
                     /label="incC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(518..1273)
                     /label="repE"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     exon            complement(518..1273)
                     /label="Translation 1273-518"
     rep_origin      1364..1583
                     /label="ori2"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     protein_bind    2003..2024
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        2039..2069
                     /label="lac promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     protein_bind    2077..2093
                     /label="lac operator"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     2101..2117
                     /label="M13 rev"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             2190..3035
                     /label="repL"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     exon            2190..3035
                     /label="Translation 2190-3035"
     misc_feature    3143..3294
                     /label="nnpp promoters"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             3334..4149
                     /label="KanR"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     exon            3334..4149
                     /label="Translation 3334-4149"
     terminator      complement(4259..4302)
                     /label="bacterial terminator"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     4320..4336
                     /label="M13 fwd"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      4337..4368
                     /label="tonB terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(4538..5620)
                     /label="lacI"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     promoter        complement(5621..5698)
                     /label="lacIq promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        6007..6025
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    6026..6050
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     RBS             6065..6087
                     /label="RBS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             6100..6129
                     /label="10xHis"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             6142..6153
                     /label="Factor Xa site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             6157..8481
                     /label="Pfu pol"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             6157..8691
                     /label="Pfu_Ssod7"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     terminator      8751..8798
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 cgctgaggtg tcaatcgtcg gagccgctga gcaataacta gcataacccc ttggggcctc
       61 taaacgggtc ttgaggggtt ttttgcatgg tcatagctgt ttcctgagag cttggcaggt
      121 gtcgcgtatg catcgatcac gtgtccacgt tctttaatag tggactcttg ttccaaactg
      181 gaacaacact cggatcgatc cggcgcgcac cgtgggaaaa actccaggta gaggtacaca
      241 cgcggatagc caattcagag taataaactg tgataatcaa ccctcatcaa tgatgacgaa
      301 ctaacccccg atatcaggtc acatgacgaa gggaaagaga aggaaatcaa ctgtgacaaa
      361 ctgccctcaa atttggcttc cttaaaaatt acagttcaaa aagtatgaga aaatccatgc
      421 aggctgaagg aaacagcaaa actgtgacaa attaccctca gtaggtcaga acaaatgtga
      481 cgaaccaccc tcaaatctgt gacagataac cctcagacta tcctgtcgtc atggaagtga
      541 tatcgcggaa ggaaaatacg atatgagtcg tctggcggcc tttctttttc tcaatgtatg
      601 agaggcgcat tggagttctg ctgttgatct cattaacaca gacttgcagg aagcggcggc
      661 ggaagtcagg catacgctgg taactttgag gcagctggta acgctctatg atccagtcga
      721 ttttcagaga cacgatgcct gagccatccg gcttacgata ctgacacagg gattcgtata
      781 aacgcatggc atacggattg gtgatttctt ttgtttcact aagccgaaac tgcgtaaacc
      841 ggttctgtaa cccgataaag aagggaatga gatatgggtt gatatgtaca ctgtaaagcc
      901 ctctggatgg actgtgcgca cgtttgataa accaaggaaa agattcatag cctttttcat
      961 caccggcatc ctcttcaggg cgataaaaaa ccacttcctt ccccgcgaaa ctcttcaatg
     1021 cctgccgtat atccttactg gcttccgcag aggtcaatcc gaatatttca gcatatttag
     1081 caacatggat ctcgcagata ccgtcatgtt cctgtagggt gccatcagat tttctgatct
     1141 ggtcaacgaa cagatacagc atacgttttt gatcccggga gagactatat gccgcctcag
     1201 tgaggtcgtt tgactggacg attcgcgggc tatttttacg tttcttgtga ttgataaccg
     1261 ctgtttccgc catgacagat ccatgtgaag tgtgacaagt ttttagattg tcacactaaa
     1321 taaaaaagag tcaataagca gggataactt tgtgaaaaaa cagcttcttc tgagggcaat
     1381 ttgtcacagg gttaagggca atttgtcaca gacaggactg tcatttgagg gtgatttgtc
     1441 acactgaaag ggcaatttgt cacaacacct tctgtagaac cagcatggat aaaggcctac
     1501 aaggcgctct aaaaaagaag atctaaaaac tataaaaaaa ataattataa aaatatcccc
     1561 gtggataagt ggataacccc aagggaagtt ttttcaggca tcgtgtgtaa gcagaatata
     1621 taagtgctgt tccctggtgc ttcctcgctc actcgaaatt cccggggata gctttatgct
     1681 tgtaaaccgt tttgtgaaaa aatttttaaa ataaaaaagg ggacctctag ggtccccaat
     1741 taattagtaa tataatctat taaaggtcat tcaaaaggtc atccaccgga tcaattcccc
     1801 tgctcgcgca ggctgggtgc caagctctcg ggtaacatca aggcccgatc cttggagccc
     1861 ttgccctccc gcacgatgat cgtgccgtga tcgaaatcca gatccttgac ccgcagttgc
     1921 aaaccctcac tgatccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     1981 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     2041 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     2101 caggaaacag ctatgaccat gattacgcca agcgcggtac ctgtcgcggc aacgcgctaa
     2161 cagacgtagt aagaaccacc agcattgtaa tgctggctaa agtcactttc ctgagctgta
     2221 taacgatgag cgattttact ttttctggct atgaattggc ctgctttgta acacactccg
     2281 gtctatcccg tagcgccggg catatcctgt cgcaatgtgc aaatctcgcg gcaacaacca
     2341 gtgaatactt cattcacaag cctcaccgcc tgatcgcggc agaaactggt tatagccaat
     2401 caaccgtcgt tcgtgcattc cgtgaagctg taaacaaagg tatcctgtct gtagagattg
     2461 ttatcggcga tcaccgtgaa cgtcgcgcta acctgtaccg gtttacacca tcctttttgg
     2521 ccttcgcaca acaagccaaa aatgcgctga tagaaagcaa attaaagatc tcttcagcgg
     2581 caaccaaggt taaagctgtt ctcgctaaga cattggcttt atttaatttt ttatccacac
     2641 ccccatgtca aaatgatacc ccctccccct gtcaggatga cgtggcaata aagaataaga
     2701 agtcacaagt taaaaaaaca aaaagatcag tttccggcgg tgccggaaca accagcctca
     2761 aaaaattgac ttcatggatc gctaaggcaa aagcaaaggc tgacaatctg cggttatcca
     2821 aaaaacgcac tcaaaaacat gagttcaagc agaaagtaga ggcggctgcg cggaaatatg
     2881 cttacctgaa gaacaagcgt tcgcctgata ttggcgggat atcaaacttc gataacctac
     2941 cgcattgcat gacggtaaac gaagctctta atgcggtttt agccaaaaat aaagataacg
     3001 aacaatgggg tataccggca ggattcagag ggtaatgaat tgctctaatt ataaccatgc
     3061 atactttcaa cacctctagt ttgccatgag gcaaactcat aggtgtcctg gtaagaggac
     3121 actgttgcca aaactggacg ccccattatt gcaattaata aacaactaac ggacaattct
     3181 acctaacaat aagtggagtt gcggccggcc gcgatccgct agcaaagcca cgttgtgtct
     3241 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     3301 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct
     3361 tgctcgcgcc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     3421 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg
     3481 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     3541 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     3601 actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta
     3661 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     3721 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgcctc
     3781 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     3841 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactgtt gccattctca
     3901 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     3961 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     4021 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     4081 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     4141 tttttctaat cagaattggt taattggttg taacactggc gagctctgcc acctgacgtc
     4201 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat
     4261 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctgcacctg caccagtcag
     4321 taaaacgacg gccagtagtc aaaagcctcc gaccggaggc ttttgacttg gttcaggtgg
     4381 agtgggagTT AATTAACATT ACTCGCATCC ATTCTCAGGC TGTCTCGTCT CGTCTCGCGC
     4441 CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC
     4501 CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG
     4561 AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG
     4621 TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGACAC GGGCAACAGC TGATTGCCCT
     4681 TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC
     4741 GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTATCT TCGGTATCGT
     4801 CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA
     4861 TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT
     4921 TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG
     4981 CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG
     5041 CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA
     5101 GATGCTCCAC GCCCAGTCGC GTACCATCTT CATGGGAGAA AATAATACTG TTGATGGGTG
     5161 TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA
     5221 TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA
     5281 GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA
     5341 CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT
     5401 GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT
     5461 GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG
     5521 TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC
     5581 CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC
     5641 TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCACCATTCG ATGGTGTCCG
     5701 GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG
     5761 AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT
     5821 CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG
     5881 TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT
     5941 GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG
     6001 CGAAATTAAT ACGACTCACT ATAGGGGAAT TGTGAGCGGA TAACAATTCC CCTCTAGAAA
     6061 TAATTTTGTT TAACTTTAAG AAGGAGATAT ACCATGGGCC ATCATCACCA TCATCATCAC
     6121 CATCATCACA GCAGCGGCCA TATCGAAGGT CGTCATATGA TTTTAGATGT GGATTACATA
     6181 ACTGAAGAAG GAAAACCTGT TATTAGGCTA TTCAAAAAAG AGAACGGAAA ATTTAAGATA
     6241 GAGCATGATA GAACTTTTAG ACCATACATT TACGCTCTGC TCAGGGATGA TTCAAAGATT
     6301 GAAGAAGTTA AGAAAATAAC GGGGGAAAGG CATGGAAAGA TTGTGAGAAT TGTTGATGTA
     6361 GAGAAGGTTG AGAAAAAGTT TCTCGGCAAG CCTATTACCG TGTGGAAACT TTATTTGGAA
     6421 CATCCCCAAG ATGTTCCCAC TATTAGAGAA AAAGTTAGAG AACATCCAGC AGTTGTGGAC
     6481 ATCTTCGAAT ACGATATTCC ATTTGCAAAG AGATACCTCA TCGACAAAGG CCTAATACCA
     6541 ATGGAGGGGG AAGAGGAACT AAAGATTCTT GCCTTCGATA TAGAAACCCT CTATCACGAA
     6601 GGAGAAGAGT TTGGAAAAGG CCCAATTATA ATGATTAGTT ATGCAGATGA AAATGAAGCA
     6661 AAGGTGATTA CTTGGAAAAA CATAGACCTT CCATACGTTG AGGTTGTATC AAGCGAGAGA
     6721 GAGATGATAA AGAGATTTCT CAGGATTATC AGGGAGAAGG ATCCTGACAT TATAGTTACT
     6781 TATAATGGAG ACTCATTCGA CTTCCCATAT TTAGCGAAAA GGGCAGAAAA ACTTGGGATT
     6841 AAATTAACCA TTGGAAGAGA TGGAAGCGAG CCCAAGATGC AGAGAATAGG CGATATGACG
     6901 GCTGTAGAAG TCAAGGGAAG AATACATTTC GACTTGTATC ATGTAATAAC AAGGACAATA
     6961 AATCTCCCAA CATACACACT AGAGGCTGTA TATGAAGCAA TTTTTGGAAA GCCAAAGGAG
     7021 AAGGTATACG CCGACGAGAT AGCAAAAGCC TGGGAAAGTG GAGAGAACCT TGAGAGAGTT
     7081 GCCAAATACT CGATGGAAGA TGCAAAGGCA ACTTATGAAC TCGGGAAAGA GTTCCTTCCA
     7141 ATGGAAATTC AGCTTTCAAG ATTAGTTGGA CAACCTTTAT GGGATGTTTC AAGGTCAAGC
     7201 ACAGGGAACC TTGTAGAGTG GTTCTTACTT AGGAAAGCCT ACGAAAGAAA CGAAGTAGCT
     7261 CCAAACAAGC CAAGTGAAGA GGAGTATCAA AGAAGGCTCA GGGAGAGCTA CACAGGTGGA
     7321 TTCGTTAAAG AGCCAGAAAA GGGGTTGTGG GAAAACATAG TATACCTAGA TTTTAGAGCC
     7381 CTATATCCCT CGATTATAAT TACCCACAAT GTTTCTCCCG ATACTCTAAA TCTTGAGGGA
     7441 TGCAAGAACT ATGATATCGC TCCTCAAGTA GGCCACAAGT TCTGCAAGGA CATCCCTGGT
     7501 TTTATACCAA GTCTCTTGGG ACATTTGTTA GAGGAAAGAC AAAAGATTAA GACAAAAATG
     7561 AAGGAAACTC AAGATCCTAT AGAAAAAATA CTCCTTGACT ATAGACAAAA AGCGATAAAA
     7621 CTCTTAGCAA ATTCTTTCTA CGGATATTAT GGCTATGCAA AAGCAAGATG GTACTGTAAG
     7681 GAGTGTGCTG AGAGCGTTAC TGCCTGGGGA AGAAAGTACA TCGAGTTAGT ATGGAAGGAG
     7741 CTCGAAGAAA AGTTTGGATT TAAAGTCCTC TACATTGACA CTGATGGTCT GTATGCAACT
     7801 ATCCCAGGAG GAGAAAGTGA GGAAATAAAG AAAAAGGCTC TGGAATTTGT AAAATACATA
     7861 AATTCAAAGC TCCCTGGACT GCTAGAGCTT GAATATGAAG GGTTCTATAA GAGGGGATTC
     7921 TTCGTTACGA AGAAGAGGTA TGCAGTAATA GATGAAGAAG GAAAAGTCAT TACTCGTGGT
     7981 TTAGAGATAG TTAGGAGAGA TTGGAGTGAA ATTGCAAAAG AAACTCAAGC TAGAGTTTTG
     8041 GAGACAATAC TAAAACACGG AGATGTTGAA GAAGCTGTGA GAATAGTAAA AGAAGTAATA
     8101 CAAAAGCTTG CCAATTATGA AATTCCACCA GAGAAGCTCG CAATATATGA GCAGATAACA
     8161 AGACCATTAC ATGAGTATAA GGCGATAGGT CCTCACGTAG CTGTTGCAAA GAAACTAGCT
     8221 GCTAAAGGAG TTAAAATAAA GCCAGGAATG GTAATTGGAT ACATAGTACT TAGAGGCGAT
     8281 GGTCCAATTA GCAATAGGGC AATTCTAGCT GAGGAATACG ATCCCAAAAA GCACAAGTAT
     8341 GACGCAGAAT ATTACATTGA GAACCAGGTT CTTCCAGCGG TACTTAGGAT ATTGGAGGGA
     8401 TTTGGATACA GAAAGGAGGA CCTCAGATAC CAAAAGACAA GACAAGTCGG CCTAACTTCC
     8461 TGGCTTAACA TTAAAAAATC CGGTACCGGC GGTGGCGGTG CAACCGTAAA GTTCAAGTAC
     8521 AAAGGCGAAG AAAAAGAGGT AGACATCTCC AAGATCAAGA AAGTATGGCG TGTGGGCAAG
     8581 ATGATCTCCT TCACCTACGA CGAGGGCGGT GGCAAGACCG GCCGTGGTGC GGTAAGCGAA
     8641 AAGGACGCGC CGAAGGAGCT GCTCCAGATG CTGGAGAAGC AGAAAAAGTG ATAACTGCTA
     8701 ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC
     8761 CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCC CCAGGATACA TAGATTACCA
     8821 CAACTCCGAG CCCTTCCACC ACTAGT
//