LOCUS Exported 4562 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..4562 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 2575..2622 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" primer_bind 4450..4466 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 4467..4498 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 1..0 /label="MMSYN1_0007" /note="GyrA: catalyzes the interconversion of DNA topological isomers" /translation="MNNENNNNDSLNENQDHYHGKISPIDISTEVRKDFLEYAMSVIVSRALPDLKDGLKPVHRRIIYAMNDLGITSDKPHKKSARIVGEVIGKYHPHGDSAVYETMVRMAQEFSYRYPLIDGHGNFGSIDGDGAAAMRYTEARLAKISNYLIKDIDMDTVPFIDNYDASEREPAYLTGYLPNLLVNGTMGIAVGMATSIPPHNLKEVVSAINAYIDNNDITIDEILNDHILGPDFPTGALMTNGSKMREGYKTGRGSVIIRAKIDFEENKKHDRFVVTEIPYQTNKAKIIEKIAELVKDKTIEGIFDIRDESNYEGIRIIIELKKDANPDVVLSKLYKYTALQSSFSINLLTLNNNLPVLLDLKTIIKNYVEFQISVIIKRSIFEKNKLTKRYHILEALHIALDNVDDVINIIKNSKTSEEAKVQLTNKYNFDEEQNKAILDMRLQRLVGLERDKITLEMTNIKERLTYLDVLINTKEEQDNVLKNQLNEIADKFGDNRRTELIDEELINIEDEELIPDLKWMILLSQEGYIRRINPDEFRIQKRGGRGVSVNAEPSDPIDIATMGKAKDWVLFFTNSGKVYRTKLYNIRSYSRTARGLPIVNFLNDLTSEDKITAILPLRNNKEKFNYLTFVTQKGMIKRTKISEFENINRNGKKAINLRDNDQLVSVFATTGQDTVFIANESGKVIRIKESVVNPQSRVGGGVKALKLEDDDVVVGAISSFKLTHITTVSNKGLFKKTPIDDYRISGRNGKGIKVMNLNQRTGKFKAIIGARETDLIMIISSDGNLIKTKVSNIPSLSRNASGVKAIRLTDNQEINAITLEYRKHGLENEDFEED" primer_bind complement(2627..2643) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(2710..3298) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3469..4329) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(4330..4434) /label="bla promoter" /note="ampicillin resistance gene promoter" CDS complement(1..0) /label="MMSYN1_0007" /note="GyrA: catalyzes the interconversion of DNA topological isomers" /translation="MNNENNNNDSLNENQDHYHGKISPIDISTEVRKDFLEYAMSVIVSRALPDLKDGLKPVHRRIIYAMNDLGITSDKPHKKSARIVGEVIGKYHPHGDSAVYETMVRMAQEFSYRYPLIDGHGNFGSIDGDGAAAMRYTEARLAKISNYLIKDIDMDTVPFIDNYDASEREPAYLTGYLPNLLVNGTMGIAVGMATSIPPHNLKEVVSAINAYIDNNDITIDEILNDHILGPDFPTGALMTNGSKMREGYKTGRGSVIIRAKIDFEENKKHDRFVVTEIPYQTNKAKIIEKIAELVKDKTIEGIFDIRDESNYEGIRIIIELKKDANPDVVLSKLYKYTALQSSFSINLLTLNNNLPVLLDLKTIIKNYVEFQISVIIKRSIFEKNKLTKRYHILEALHIALDNVDDVINIIKNSKTSEEAKVQLTNKYNFDEEQNKAILDMRLQRLVGLERDKITLEMTNIKERLTYLDVLINTKEEQDNVLKNQLNEIADKFGDNRRTELIDEELINIEDEELIPDLKWMILLSQEGYIRRINPDEFRIQKRGGRGVSVNAEPSDPIDIATMGKAKDWVLFFTNSGKVYRTKLYNIRSYSRTARGLPIVNFLNDLTSEDKITAILPLRNNKEKFNYLTFVTQKGMIKRTKISEFENINRNGKKAINLRDNDQLVSVFATTGQDTVFIANESGKVIRIKESVVNPQSRVGGGVKALKLEDDDVVVGAISSFKLTHITTVSNKGLFKKTPIDDYRISGRNGKGIKVMNLNQRTGKFKAIIGARETDLIMIISSDGNLIKTKVSNIPSLSRNASGVKAIRLTDNQEINAITLEYRKHGLENEDFEED" ORIGIN 1 ATGAATAACG AAAATAACAA CAATGACTCG CTTAACGAGA ATCAGGATCA CTACCACGGT 61 AAGATTAGTC CTATTGATAT TTCTACCGAA GTGCGCAAAG ACTTTTTAGA GTACGCCATG 121 TCTGTAATCG TGTCCCGTGC ATTACCAGAT CTTAAGGACG GGTTAAAACC TGTACACCGC 181 CGCATCATCT ATGCCATGAA TGACTTGGGG ATCACGTCTG ACAAACCCCA CAAAAAGTCA 241 GCACGTATTG TCGGTGAGGT CATTGGTAAG TATCACCCTC ACGGAGATTC GGCCGTGTAT 301 GAGACTATGG TACGTATGGC TCAAGAGTTC TCTTACCGCT ACCCACTGAT CGACGGCCAT 361 GGAAACTTCG GGTCTATCGA CGGGGATGGT GCCGCCGCCA TGCGCTACAC CGAAGCTCGT 421 TTGGCTAAGA TCAGCAACTA CTTGATTAAG GACATTGATA TGGACACGGT GCCGTTTATC 481 GACAATTACG ATGCGTCAGA GCGCGAACCC GCTTATCTTA CCGGATACTT GCCCAATTTG 541 TTAGTGAATG GGACAATGGG GATCGCCGTA GGGATGGCTA CGTCGATCCC TCCACACAAT 601 TTGAAAGAAG TTGTCTCGGC CATTAATGCT TACATTGATA ATAACGATAT TACAATTGAT 661 GAAATCCTTA ACGATCACAT TTTGGGGCCC GATTTCCCGA CAGGGGCTCT TATGACCAAT 721 GGATCCAAAA TGCGTGAGGG ATATAAGACA GGGCGTGGTA GTGTTATCAT CCGTGCGAAG 781 ATCGACTTTG AGGAGAACAA GAAGCACGAT CGCTTTGTCG TCACGGAAAT CCCTTATCAA 841 ACGAACAAGG CGAAAATCAT TGAGAAAATC GCCGAATTGG TCAAGGACAA GACCATCGAG 901 GGAATCTTCG ATATTCGTGA CGAGTCGAAT TATGAAGGTA TTCGCATCAT CATCGAGTTG 961 AAGAAAGATG CTAACCCTGA CGTGGTATTG TCAAAGTTAT ACAAGTATAC GGCCCTTCAA 1021 TCTTCTTTTT CAATCAATCT TTTAACCCTG AATAACAATC TGCCTGTTCT TCTTGATCTT 1081 AAGACAATCA TCAAGAATTA TGTAGAATTC CAGATTAGCG TCATCATCAA GCGTAGCATT 1141 TTCGAAAAGA ATAAGTTAAC TAAACGCTAC CACATTTTAG AGGCCCTTCA TATCGCTCTT 1201 GACAATGTAG ATGACGTTAT CAACATTATT AAAAACTCTA AGACGAGTGA GGAGGCTAAA 1261 GTGCAATTAA CTAACAAGTA TAACTTTGAC GAGGAGCAAA ACAAAGCAAT CTTGGATATG 1321 CGTCTGCAAC GTTTAGTTGG ATTGGAACGT GACAAAATTA CACTTGAGAT GACCAATATC 1381 AAAGAGCGCT TAACTTATTT AGACGTTTTG ATTAACACTA AAGAGGAGCA GGACAACGTA 1441 CTGAAGAATC AGCTGAATGA GATCGCCGAC AAATTTGGGG ATAACCGTCG TACGGAACTT 1501 ATTGATGAGG AGCTTATCAA CATCGAGGAC GAGGAATTGA TCCCTGACCT TAAATGGATG 1561 ATTTTGCTGT CTCAAGAGGG TTATATTCGT CGCATCAACC CAGACGAATT TCGCATTCAG 1621 AAACGCGGTG GTCGTGGCGT ATCCGTAAAT GCTGAACCGT CCGATCCAAT TGATATTGCG 1681 ACCATGGGCA AGGCTAAGGA CTGGGTGTTA TTTTTCACAA ATTCGGGCAA AGTCTACCGT 1741 ACAAAGTTAT ACAATATCCG TTCATATTCG CGTACCGCTC GCGGCCTTCC GATCGTCAAT 1801 TTTCTTAATG ACTTAACAAG TGAGGACAAA ATTACGGCCA TTTTACCACT TCGCAATAAC 1861 AAAGAAAAGT TTAACTACCT GACTTTCGTT ACACAAAAGG GGATGATTAA ACGTACGAAG 1921 ATTAGTGAGT TTGAGAATAT CAACCGTAAT GGCAAGAAGG CAATCAACCT TCGTGATAAT 1981 GACCAATTAG TCTCGGTGTT CGCCACGACG GGACAAGATA CAGTGTTTAT CGCTAACGAG 2041 TCGGGAAAGG TAATTCGCAT TAAAGAAAGC GTGGTTAATC CCCAGTCGCG CGTGGGGGGC 2101 GGGGTGAAAG CACTGAAATT AGAGGACGAT GATGTGGTTG TGGGTGCGAT TAGTTCGTTC 2161 AAGTTAACGC ATATTACCAC CGTTTCTAAT AAAGGACTTT TCAAAAAGAC GCCTATCGAC 2221 GACTATCGTA TCAGTGGGCG TAATGGTAAA GGCATCAAGG TAATGAATCT TAATCAGCGT 2281 ACCGGCAAGT TCAAAGCTAT TATCGGCGCC CGTGAGACTG ATCTGATTAT GATCATCAGC 2341 TCGGATGGGA ATTTGATCAA GACCAAAGTT AGCAATATCC CCTCTCTTTC ACGTAATGCA 2401 TCTGGTGTCA AAGCCATTCG TTTAACAGAT AACCAAGAAA TTAATGCTAT TACGCTGGAA 2461 TATCGCAAAC ACGGCCTGGA GAATGAGGAT TTCGAGGAGG ATTGAAGAGC TTAGAGACCG 2521 GTACTAGTTC CTGCAGGGCT CGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA 2581 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC 2641 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA 2701 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 2761 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 2821 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 2881 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 2941 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 3001 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 3061 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 3121 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 3181 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 3241 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 3301 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 3361 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 3421 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT 3481 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT 3541 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT 3601 GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG 3661 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG 3721 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT 3781 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC 3841 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT 3901 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC 3961 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA 4021 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC 4081 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA 4141 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA 4201 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG 4261 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG 4321 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT 4381 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG 4441 CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG 4501 GTCAGGTGGA GTGAATTCGC GGCCGCTTCT AGAGGCTCTT CGTAATTACG AAGAGGTCTC 4561 TA //