LOCUS       Exported     4562 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4562
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2575..2622
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     4450..4466
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      4467..4498
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0007"
                     /note="GyrA: catalyzes the interconversion of DNA topological isomers"
                     /translation="MNNENNNNDSLNENQDHYHGKISPIDISTEVRKDFLEYAMSVIVSRALPDLKDGLKPVHRRIIYAMNDLGITSDKPHKKSARIVGEVIGKYHPHGDSAVYETMVRMAQEFSYRYPLIDGHGNFGSIDGDGAAAMRYTEARLAKISNYLIKDIDMDTVPFIDNYDASEREPAYLTGYLPNLLVNGTMGIAVGMATSIPPHNLKEVVSAINAYIDNNDITIDEILNDHILGPDFPTGALMTNGSKMREGYKTGRGSVIIRAKIDFEENKKHDRFVVTEIPYQTNKAKIIEKIAELVKDKTIEGIFDIRDESNYEGIRIIIELKKDANPDVVLSKLYKYTALQSSFSINLLTLNNNLPVLLDLKTIIKNYVEFQISVIIKRSIFEKNKLTKRYHILEALHIALDNVDDVINIIKNSKTSEEAKVQLTNKYNFDEEQNKAILDMRLQRLVGLERDKITLEMTNIKERLTYLDVLINTKEEQDNVLKNQLNEIADKFGDNRRTELIDEELINIEDEELIPDLKWMILLSQEGYIRRINPDEFRIQKRGGRGVSVNAEPSDPIDIATMGKAKDWVLFFTNSGKVYRTKLYNIRSYSRTARGLPIVNFLNDLTSEDKITAILPLRNNKEKFNYLTFVTQKGMIKRTKISEFENINRNGKKAINLRDNDQLVSVFATTGQDTVFIANESGKVIRIKESVVNPQSRVGGGVKALKLEDDDVVVGAISSFKLTHITTVSNKGLFKKTPIDDYRISGRNGKGIKVMNLNQRTGKFKAIIGARETDLIMIISSDGNLIKTKVSNIPSLSRNASGVKAIRLTDNQEINAITLEYRKHGLENEDFEED"
     primer_bind     complement(2627..2643)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2710..3298)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(3469..4329)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(4330..4434)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0007"
                     /note="GyrA: catalyzes the interconversion of DNA topological isomers"
                     /translation="MNNENNNNDSLNENQDHYHGKISPIDISTEVRKDFLEYAMSVIVSRALPDLKDGLKPVHRRIIYAMNDLGITSDKPHKKSARIVGEVIGKYHPHGDSAVYETMVRMAQEFSYRYPLIDGHGNFGSIDGDGAAAMRYTEARLAKISNYLIKDIDMDTVPFIDNYDASEREPAYLTGYLPNLLVNGTMGIAVGMATSIPPHNLKEVVSAINAYIDNNDITIDEILNDHILGPDFPTGALMTNGSKMREGYKTGRGSVIIRAKIDFEENKKHDRFVVTEIPYQTNKAKIIEKIAELVKDKTIEGIFDIRDESNYEGIRIIIELKKDANPDVVLSKLYKYTALQSSFSINLLTLNNNLPVLLDLKTIIKNYVEFQISVIIKRSIFEKNKLTKRYHILEALHIALDNVDDVINIIKNSKTSEEAKVQLTNKYNFDEEQNKAILDMRLQRLVGLERDKITLEMTNIKERLTYLDVLINTKEEQDNVLKNQLNEIADKFGDNRRTELIDEELINIEDEELIPDLKWMILLSQEGYIRRINPDEFRIQKRGGRGVSVNAEPSDPIDIATMGKAKDWVLFFTNSGKVYRTKLYNIRSYSRTARGLPIVNFLNDLTSEDKITAILPLRNNKEKFNYLTFVTQKGMIKRTKISEFENINRNGKKAINLRDNDQLVSVFATTGQDTVFIANESGKVIRIKESVVNPQSRVGGGVKALKLEDDDVVVGAISSFKLTHITTVSNKGLFKKTPIDDYRISGRNGKGIKVMNLNQRTGKFKAIIGARETDLIMIISSDGNLIKTKVSNIPSLSRNASGVKAIRLTDNQEINAITLEYRKHGLENEDFEED"
ORIGIN
        1 ATGAATAACG AAAATAACAA CAATGACTCG CTTAACGAGA ATCAGGATCA CTACCACGGT
       61 AAGATTAGTC CTATTGATAT TTCTACCGAA GTGCGCAAAG ACTTTTTAGA GTACGCCATG
      121 TCTGTAATCG TGTCCCGTGC ATTACCAGAT CTTAAGGACG GGTTAAAACC TGTACACCGC
      181 CGCATCATCT ATGCCATGAA TGACTTGGGG ATCACGTCTG ACAAACCCCA CAAAAAGTCA
      241 GCACGTATTG TCGGTGAGGT CATTGGTAAG TATCACCCTC ACGGAGATTC GGCCGTGTAT
      301 GAGACTATGG TACGTATGGC TCAAGAGTTC TCTTACCGCT ACCCACTGAT CGACGGCCAT
      361 GGAAACTTCG GGTCTATCGA CGGGGATGGT GCCGCCGCCA TGCGCTACAC CGAAGCTCGT
      421 TTGGCTAAGA TCAGCAACTA CTTGATTAAG GACATTGATA TGGACACGGT GCCGTTTATC
      481 GACAATTACG ATGCGTCAGA GCGCGAACCC GCTTATCTTA CCGGATACTT GCCCAATTTG
      541 TTAGTGAATG GGACAATGGG GATCGCCGTA GGGATGGCTA CGTCGATCCC TCCACACAAT
      601 TTGAAAGAAG TTGTCTCGGC CATTAATGCT TACATTGATA ATAACGATAT TACAATTGAT
      661 GAAATCCTTA ACGATCACAT TTTGGGGCCC GATTTCCCGA CAGGGGCTCT TATGACCAAT
      721 GGATCCAAAA TGCGTGAGGG ATATAAGACA GGGCGTGGTA GTGTTATCAT CCGTGCGAAG
      781 ATCGACTTTG AGGAGAACAA GAAGCACGAT CGCTTTGTCG TCACGGAAAT CCCTTATCAA
      841 ACGAACAAGG CGAAAATCAT TGAGAAAATC GCCGAATTGG TCAAGGACAA GACCATCGAG
      901 GGAATCTTCG ATATTCGTGA CGAGTCGAAT TATGAAGGTA TTCGCATCAT CATCGAGTTG
      961 AAGAAAGATG CTAACCCTGA CGTGGTATTG TCAAAGTTAT ACAAGTATAC GGCCCTTCAA
     1021 TCTTCTTTTT CAATCAATCT TTTAACCCTG AATAACAATC TGCCTGTTCT TCTTGATCTT
     1081 AAGACAATCA TCAAGAATTA TGTAGAATTC CAGATTAGCG TCATCATCAA GCGTAGCATT
     1141 TTCGAAAAGA ATAAGTTAAC TAAACGCTAC CACATTTTAG AGGCCCTTCA TATCGCTCTT
     1201 GACAATGTAG ATGACGTTAT CAACATTATT AAAAACTCTA AGACGAGTGA GGAGGCTAAA
     1261 GTGCAATTAA CTAACAAGTA TAACTTTGAC GAGGAGCAAA ACAAAGCAAT CTTGGATATG
     1321 CGTCTGCAAC GTTTAGTTGG ATTGGAACGT GACAAAATTA CACTTGAGAT GACCAATATC
     1381 AAAGAGCGCT TAACTTATTT AGACGTTTTG ATTAACACTA AAGAGGAGCA GGACAACGTA
     1441 CTGAAGAATC AGCTGAATGA GATCGCCGAC AAATTTGGGG ATAACCGTCG TACGGAACTT
     1501 ATTGATGAGG AGCTTATCAA CATCGAGGAC GAGGAATTGA TCCCTGACCT TAAATGGATG
     1561 ATTTTGCTGT CTCAAGAGGG TTATATTCGT CGCATCAACC CAGACGAATT TCGCATTCAG
     1621 AAACGCGGTG GTCGTGGCGT ATCCGTAAAT GCTGAACCGT CCGATCCAAT TGATATTGCG
     1681 ACCATGGGCA AGGCTAAGGA CTGGGTGTTA TTTTTCACAA ATTCGGGCAA AGTCTACCGT
     1741 ACAAAGTTAT ACAATATCCG TTCATATTCG CGTACCGCTC GCGGCCTTCC GATCGTCAAT
     1801 TTTCTTAATG ACTTAACAAG TGAGGACAAA ATTACGGCCA TTTTACCACT TCGCAATAAC
     1861 AAAGAAAAGT TTAACTACCT GACTTTCGTT ACACAAAAGG GGATGATTAA ACGTACGAAG
     1921 ATTAGTGAGT TTGAGAATAT CAACCGTAAT GGCAAGAAGG CAATCAACCT TCGTGATAAT
     1981 GACCAATTAG TCTCGGTGTT CGCCACGACG GGACAAGATA CAGTGTTTAT CGCTAACGAG
     2041 TCGGGAAAGG TAATTCGCAT TAAAGAAAGC GTGGTTAATC CCCAGTCGCG CGTGGGGGGC
     2101 GGGGTGAAAG CACTGAAATT AGAGGACGAT GATGTGGTTG TGGGTGCGAT TAGTTCGTTC
     2161 AAGTTAACGC ATATTACCAC CGTTTCTAAT AAAGGACTTT TCAAAAAGAC GCCTATCGAC
     2221 GACTATCGTA TCAGTGGGCG TAATGGTAAA GGCATCAAGG TAATGAATCT TAATCAGCGT
     2281 ACCGGCAAGT TCAAAGCTAT TATCGGCGCC CGTGAGACTG ATCTGATTAT GATCATCAGC
     2341 TCGGATGGGA ATTTGATCAA GACCAAAGTT AGCAATATCC CCTCTCTTTC ACGTAATGCA
     2401 TCTGGTGTCA AAGCCATTCG TTTAACAGAT AACCAAGAAA TTAATGCTAT TACGCTGGAA
     2461 TATCGCAAAC ACGGCCTGGA GAATGAGGAT TTCGAGGAGG ATTGAAGAGC TTAGAGACCG
     2521 GTACTAGTTC CTGCAGGGCT CGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA
     2581 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC
     2641 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA
     2701 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     2761 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     2821 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     2881 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     2941 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     3001 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     3061 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     3121 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     3181 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     3241 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     3301 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG
     3361 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG
     3421 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT
     3481 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT
     3541 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT
     3601 GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG
     3661 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG
     3721 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT
     3781 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC
     3841 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT
     3901 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC
     3961 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA
     4021 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC
     4081 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA
     4141 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA
     4201 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG
     4261 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG
     4321 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT
     4381 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG
     4441 CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG
     4501 GTCAGGTGGA GTGAATTCGC GGCCGCTTCT AGAGGCTCTT CGTAATTACG AAGAGGTCTC
     4561 TA
//