LOCUS       Exported     3035 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3035
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1048..1095
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2923..2939
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      2940..2971
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0008"
                     /note=""
                     /translation="MTILFNTSILTWALALVGVLLFASLSSLVSEKAGVVNIAVEGMMIIGALVVSILGTYLTSNDNKSNYTQIPIVLLAGVITAVFALLHAFPAITLKANQIISGTAINILALGLGIFLSTSNWFGKQSQVIASGYSSIDVINITKVVNGKTQQVASMLPIWTIIAIILAIGLFVFFKYTKQGMRYAMVGENPNAIDAAGISVTKYRYLAVILSGFLAGVGGGVFVVTAVSGGGLFSGNMLGYGFLGIAIMIFGQWRISFIVIGSIIFSWLFALGQQIGTLSTNKTIQAISTLFNTLPFVLTILAMVAFSKTSRAPAAVGVPFDKAKR"
     primer_bind     complement(1100..1116)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1183..1771)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1942..2802)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2803..2907)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0008"
                     /note=""
                     /translation="MTILFNTSILTWALALVGVLLFASLSSLVSEKAGVVNIAVEGMMIIGALVVSILGTYLTSNDNKSNYTQIPIVLLAGVITAVFALLHAFPAITLKANQIISGTAINILALGLGIFLSTSNWFGKQSQVIASGYSSIDVINITKVVNGKTQQVASMLPIWTIIAIILAIGLFVFFKYTKQGMRYAMVGENPNAIDAAGISVTKYRYLAVILSGFLAGVGGGVFVVTAVSGGGLFSGNMLGYGFLGIAIMIFGQWRISFIVIGSIIFSWLFALGQQIGTLSTNKTIQAISTLFNTLPFVLTILAMVAFSKTSRAPAAVGVPFDKAKR"
ORIGIN
        1 ATGACCATTC TGTTTAATAC ATCAATTTTG ACTTGGGCGC TTGCTCTGGT CGGTGTACTG
       61 CTGTTTGCTA GCTTATCCTC CCTTGTATCC GAGAAAGCTG GGGTAGTGAA TATCGCAGTA
      121 GAAGGGATGA TGATTATCGG GGCGTTGGTC GTTAGTATCC TGGGGACATA TCTGACATCG
      181 AATGATAACA AAAGTAACTA CACCCAGATC CCCATTGTCC TGCTTGCGGG CGTAATCACA
      241 GCGGTATTTG CCTTGTTACA CGCATTTCCG GCTATTACAT TAAAAGCCAA TCAGATTATT
      301 TCTGGGACAG CTATCAACAT CTTAGCCTTG GGGTTAGGTA TCTTTTTGTC CACCTCTAAC
      361 TGGTTTGGGA AACAGTCACA GGTTATTGCC AGTGGCTATT CGTCCATTGA CGTTATTAAC
      421 ATTACTAAAG TCGTTAACGG AAAGACCCAA CAGGTTGCGA GCATGTTGCC GATCTGGACG
      481 ATTATCGCGA TTATTTTGGC GATTGGGCTG TTCGTGTTCT TCAAGTACAC AAAACAAGGG
      541 ATGCGCTATG CAATGGTGGG GGAAAACCCG AATGCGATCG ATGCGGCTGG TATTTCAGTC
      601 ACGAAGTACC GTTACCTTGC AGTCATTCTG TCCGGGTTTC TGGCAGGAGT TGGTGGCGGG
      661 GTATTCGTAG TCACCGCAGT GTCTGGAGGC GGTTTGTTCT CGGGTAATAT GTTAGGCTAT
      721 GGATTTCTGG GTATTGCGAT CATGATTTTT GGTCAATGGC GTATCAGTTT CATTGTCATC
      781 GGGTCTATCA TTTTCAGCTG GCTGTTCGCT CTTGGCCAGC AAATTGGAAC TTTGTCAACC
      841 AATAAGACGA TTCAAGCAAT CTCAACGCTT TTTAACACTC TGCCTTTCGT TCTTACTATT
      901 TTAGCTATGG TCGCCTTTAG TAAAACGAGC CGCGCCCCCG CAGCTGTGGG TGTCCCTTTT
      961 GACAAGGCAA AGCGCTGAAG AGCTTAGAGA CCGGTACTAG TTCCTGCAGG GCTCGAGGTG
     1021 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC
     1081 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC
     1141 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC
     1201 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     1261 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     1321 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     1381 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     1441 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     1501 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     1561 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     1621 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     1681 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     1741 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC
     1801 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG
     1861 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA
     1921 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT
     1981 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG
     2041 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC
     2101 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC
     2161 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC
     2221 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC
     2281 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC
     2341 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA
     2401 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT
     2461 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA
     2521 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA
     2581 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG
     2641 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC
     2701 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC
     2761 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA
     2821 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA
     2881 GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA
     2941 GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTCAGGT GGAGTGAATT CGCGGCCGCT
     3001 TCTAGAGGCT CTTCGTAATT ACGAAGAGGT CTCTA
//