LOCUS Exported 3035 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3035 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1048..1095 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2923..2939 /note="common sequencing primer, one of multiple similar variants" /label="M13 for" terminator 2940..2971 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 1..0 /label="MMSYN1_0008" /note="" /translation="MTILFNTSILTWALALVGVLLFASLSSLVSEKAGVVNIAVEGMMIIGALVVSILGTYLTSNDNKSNYTQIPIVLLAGVITAVFALLHAFPAITLKANQIISGTAINILALGLGIFLSTSNWFGKQSQVIASGYSSIDVINITKVVNGKTQQVASMLPIWTIIAIILAIGLFVFFKYTKQGMRYAMVGENPNAIDAAGISVTKYRYLAVILSGFLAGVGGGVFVVTAVSGGGLFSGNMLGYGFLGIAIMIFGQWRISFIVIGSIIFSWLFALGQQIGTLSTNKTIQAISTLFNTLPFVLTILAMVAFSKTSRAPAAVGVPFDKAKR" primer_bind complement(1100..1116) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1183..1771) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1942..2802) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2803..2907) /label="bla promoter" /note="ampicillin resistance gene promoter" CDS complement(1..0) /label="MMSYN1_0008" /note="" /translation="MTILFNTSILTWALALVGVLLFASLSSLVSEKAGVVNIAVEGMMIIGALVVSILGTYLTSNDNKSNYTQIPIVLLAGVITAVFALLHAFPAITLKANQIISGTAINILALGLGIFLSTSNWFGKQSQVIASGYSSIDVINITKVVNGKTQQVASMLPIWTIIAIILAIGLFVFFKYTKQGMRYAMVGENPNAIDAAGISVTKYRYLAVILSGFLAGVGGGVFVVTAVSGGGLFSGNMLGYGFLGIAIMIFGQWRISFIVIGSIIFSWLFALGQQIGTLSTNKTIQAISTLFNTLPFVLTILAMVAFSKTSRAPAAVGVPFDKAKR" ORIGIN 1 ATGACCATTC TGTTTAATAC ATCAATTTTG ACTTGGGCGC TTGCTCTGGT CGGTGTACTG 61 CTGTTTGCTA GCTTATCCTC CCTTGTATCC GAGAAAGCTG GGGTAGTGAA TATCGCAGTA 121 GAAGGGATGA TGATTATCGG GGCGTTGGTC GTTAGTATCC TGGGGACATA TCTGACATCG 181 AATGATAACA AAAGTAACTA CACCCAGATC CCCATTGTCC TGCTTGCGGG CGTAATCACA 241 GCGGTATTTG CCTTGTTACA CGCATTTCCG GCTATTACAT TAAAAGCCAA TCAGATTATT 301 TCTGGGACAG CTATCAACAT CTTAGCCTTG GGGTTAGGTA TCTTTTTGTC CACCTCTAAC 361 TGGTTTGGGA AACAGTCACA GGTTATTGCC AGTGGCTATT CGTCCATTGA CGTTATTAAC 421 ATTACTAAAG TCGTTAACGG AAAGACCCAA CAGGTTGCGA GCATGTTGCC GATCTGGACG 481 ATTATCGCGA TTATTTTGGC GATTGGGCTG TTCGTGTTCT TCAAGTACAC AAAACAAGGG 541 ATGCGCTATG CAATGGTGGG GGAAAACCCG AATGCGATCG ATGCGGCTGG TATTTCAGTC 601 ACGAAGTACC GTTACCTTGC AGTCATTCTG TCCGGGTTTC TGGCAGGAGT TGGTGGCGGG 661 GTATTCGTAG TCACCGCAGT GTCTGGAGGC GGTTTGTTCT CGGGTAATAT GTTAGGCTAT 721 GGATTTCTGG GTATTGCGAT CATGATTTTT GGTCAATGGC GTATCAGTTT CATTGTCATC 781 GGGTCTATCA TTTTCAGCTG GCTGTTCGCT CTTGGCCAGC AAATTGGAAC TTTGTCAACC 841 AATAAGACGA TTCAAGCAAT CTCAACGCTT TTTAACACTC TGCCTTTCGT TCTTACTATT 901 TTAGCTATGG TCGCCTTTAG TAAAACGAGC CGCGCCCCCG CAGCTGTGGG TGTCCCTTTT 961 GACAAGGCAA AGCGCTGAAG AGCTTAGAGA CCGGTACTAG TTCCTGCAGG GCTCGAGGTG 1021 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC 1081 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC 1141 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC 1201 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 1261 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 1321 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 1381 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 1441 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 1501 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 1561 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 1621 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 1681 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 1741 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 1801 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 1861 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 1921 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT 1981 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG 2041 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC 2101 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC 2161 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC 2221 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC 2281 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC 2341 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA 2401 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT 2461 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA 2521 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA 2581 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG 2641 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC 2701 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC 2761 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA 2821 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA 2881 GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA 2941 GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTCAGGT GGAGTGAATT CGCGGCCGCT 3001 TCTAGAGGCT CTTCGTAATT ACGAAGAGGT CTCTA //