LOCUS       Exported     4625 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4625
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2638..2685
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     4513..4529
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      4530..4561
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /note="RnsC: nucleoside ABC transporter, permease protein"
                     /translation="MQSKISWTLSKKSKYLFGSDTTKTKLKYIKGSIFSIIVGFIVSGIFLSFLNINPFTYFALLFGINFDKNFYQISLNWMAVYIVAGLSMAIAFKSGVFNIGASGQILTATSVATIVFFSISGKDASSITPFMIILMLITCIISAAFIAFIAGILKALFNIHEVVSTILLNWSVFYIFKWFFGRYQDFSSGLSYTSKNIPSDQLSIGSNTVIIPLLIALICVIIIWILFSKTVLGFKLKAVGSSITGSKYIGINVKRQIITSLTLSGAVAGIAGFLSMFTVSPNNFFASNSLPTLGFDAIAVSLVAFNNPIGIIAIGWLWAIIKTGGGPISSLYSISTQISGLISGILIYFTAIVSVFIAFKPWELLKNKYNLYTSKVNREIYWKLKLYTFKLRLKKIFLIFTKDYKQEVNNKYQIYTKQNQITNKTSNPHLFWNGRKNIEIELKNDLKQSIYLVKSKIDEIKKFVDQDKNSLNVSGLKNDLNKQVNLLASKYIQNLRDLDLELADHKYKIQKITSSILNEYQTNIKQAKKTHRLKIQQIKVFKESQIGIITYKFDSHNNIIEIKANKLKTIAQLKEQIKNIKSELRLEKQISNLNKSSTQTNNSEKLEKIKQLKEQIKVVRKQANAKILEQKNKYKNQKNSVKQEQVQLQDILHQYNNYLNKEKDRFKESKKAALVLKQKRLQAIDMNLSQSDVNKAVSLLNDLKTLITDNLDLNLNKQAIKSNQKTLKNALEIKSKIDEVLTQNIISEYDAPEHIKQKSKISLTTFKLITNLKKQISYVITRVEEQELIDKYQQWISQAKQVVQDEKNNYEQIIKKAPRKNLADLENLFNLEKSLKEQTNLKILNLTNTMLKETK"
                     /label="MMSYN1_0009"
     primer_bind     complement(2690..2706)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2773..3361)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(3532..4392)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(4393..4497)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0009"
                     /note="RnsC: nucleoside ABC transporter, permease protein"
                     /translation="MQSKISWTLSKKSKYLFGSDTTKTKLKYIKGSIFSIIVGFIVSGIFLSFLNINPFTYFALLFGINFDKNFYQISLNWMAVYIVAGLSMAIAFKSGVFNIGASGQILTATSVATIVFFSISGKDASSITPFMIILMLITCIISAAFIAFIAGILKALFNIHEVVSTILLNWSVFYIFKWFFGRYQDFSSGLSYTSKNIPSDQLSIGSNTVIIPLLIALICVIIIWILFSKTVLGFKLKAVGSSITGSKYIGINVKRQIITSLTLSGAVAGIAGFLSMFTVSPNNFFASNSLPTLGFDAIAVSLVAFNNPIGIIAIGWLWAIIKTGGGPISSLYSISTQISGLISGILIYFTAIVSVFIAFKPWELLKNKYNLYTSKVNREIYWKLKLYTFKLRLKKIFLIFTKDYKQEVNNKYQIYTKQNQITNKTSNPHLFWNGRKNIEIELKNDLKQSIYLVKSKIDEIKKFVDQDKNSLNVSGLKNDLNKQVNLLASKYIQNLRDLDLELADHKYKIQKITSSILNEYQTNIKQAKKTHRLKIQQIKVFKESQIGIITYKFDSHNNIIEIKANKLKTIAQLKEQIKNIKSELRLEKQISNLNKSSTQTNNSEKLEKIKQLKEQIKVVRKQANAKILEQKNKYKNQKNSVKQEQVQLQDILHQYNNYLNKEKDRFKESKKAALVLKQKRLQAIDMNLSQSDVNKAVSLLNDLKTLITDNLDLNLNKQAIKSNQKTLKNALEIKSKIDEVLTQNIISEYDAPEHIKQKSKISLTTFKLITNLKKQISYVITRVEEQELIDKYQQWISQAKQVVQDEKNNYEQIIKKAPRKNLADLENLFNLEKSLKEQTNLKILNLTNTMLKETK"
ORIGIN
        1 ATGCAGAGTA AGATTAGTTG GACATTAAGC AAGAAAAGTA AGTATCTTTT CGGCTCTGAC
       61 ACCACCAAGA CCAAGTTAAA GTACATTAAG GGCAGCATTT TCTCTATTAT TGTCGGCTTC
      121 ATTGTAAGCG GTATCTTCCT GTCCTTCTTG AATATCAATC CTTTTACGTA CTTTGCCCTG
      181 TTGTTTGGAA TCAACTTCGA TAAGAATTTT TATCAGATCT CCTTGAACTG GATGGCCGTA
      241 TATATCGTGG CAGGCTTAAG TATGGCCATT GCTTTCAAAA GCGGCGTGTT TAACATTGGG
      301 GCCTCAGGGC AAATTTTGAC TGCAACATCG GTGGCCACCA TTGTATTCTT TTCCATCAGT
      361 GGTAAGGATG CCTCGAGTAT CACACCCTTT ATGATCATCT TAATGTTGAT CACGTGTATT
      421 ATCTCTGCTG CGTTTATTGC ATTTATTGCC GGGATTCTTA AGGCACTTTT TAATATCCAC
      481 GAGGTCGTAT CGACGATTTT ACTTAATTGG TCCGTATTTT ATATCTTCAA GTGGTTTTTC
      541 GGGCGTTACC AGGACTTCTC TTCGGGATTG TCATATACAT CCAAAAACAT CCCATCCGAC
      601 CAGCTTTCGA TCGGTTCCAA TACTGTAATT ATTCCGTTAT TGATCGCGTT GATCTGTGTA
      661 ATCATTATTT GGATCTTATT TTCAAAGACT GTACTTGGCT TTAAGCTTAA GGCCGTAGGA
      721 AGTTCGATTA CTGGTTCGAA GTATATTGGT ATCAACGTAA AACGCCAGAT CATTACAAGC
      781 CTTACGCTTT CAGGTGCCGT AGCCGGTATC GCGGGTTTCC TTTCGATGTT CACTGTAAGT
      841 CCTAACAACT TCTTCGCCAG CAACTCTTTG CCTACTTTGG GGTTCGATGC CATTGCCGTA
      901 TCGTTGGTGG CGTTCAACAA TCCCATCGGG ATCATTGCGA TTGGATGGCT GTGGGCCATC
      961 ATTAAGACTG GAGGAGGCCC AATCTCCAGC CTGTACTCGA TCTCGACTCA GATTTCCGGA
     1021 TTGATTTCGG GGATCTTAAT TTATTTTACC GCGATTGTCT CAGTCTTTAT TGCCTTCAAG
     1081 CCATGGGAAC TTCTTAAGAA TAAATACAAT TTGTACACGT CTAAAGTGAA TCGTGAGATT
     1141 TACTGGAAGC TTAAGCTTTA CACATTTAAG TTGCGCCTCA AGAAAATCTT TTTAATCTTT
     1201 ACTAAAGACT ATAAGCAGGA GGTAAACAAC AAGTATCAAA TTTACACGAA ACAGAATCAG
     1261 ATCACCAACA AGACGAGCAA TCCGCACTTA TTTTGGAATG GACGTAAGAA TATTGAAATC
     1321 GAACTGAAAA ATGATCTGAA GCAGTCAATC TATCTTGTCA AGAGCAAGAT TGACGAGATT
     1381 AAGAAGTTTG TAGATCAAGA CAAAAATTCG TTGAACGTGT CCGGCTTGAA GAACGACCTG
     1441 AACAAGCAAG TGAACTTATT GGCTTCAAAA TACATCCAGA ATTTACGCGA TTTAGACTTA
     1501 GAATTAGCCG ATCATAAGTA CAAGATCCAG AAGATCACAT CTAGCATCCT GAATGAGTAC
     1561 CAGACAAACA TTAAGCAAGC GAAGAAAACA CACCGCTTGA AGATCCAGCA GATCAAGGTG
     1621 TTCAAGGAAT CCCAGATCGG CATTATCACG TATAAATTCG ACTCGCACAA CAACATTATT
     1681 GAGATCAAAG CTAATAAGCT GAAAACAATC GCTCAACTGA AGGAACAGAT CAAAAATATC
     1741 AAGAGTGAAC TGCGTTTAGA GAAGCAGATT TCTAACCTGA ACAAATCCTC TACCCAGACT
     1801 AATAATTCAG AGAAGTTGGA AAAGATTAAA CAATTAAAAG AGCAAATTAA AGTGGTGCGT
     1861 AAACAGGCGA ATGCGAAGAT TTTGGAACAG AAAAATAAGT ATAAAAATCA GAAGAATTCG
     1921 GTGAAGCAAG AACAGGTCCA ATTACAAGAT ATCTTGCACC AATACAACAA TTACCTTAAT
     1981 AAAGAGAAGG ATCGTTTTAA AGAGTCGAAA AAAGCAGCAC TTGTTTTAAA GCAGAAGCGC
     2041 CTTCAAGCTA TTGATATGAA TTTAAGTCAA TCCGACGTAA ATAAAGCCGT GAGTCTGCTT
     2101 AACGATCTGA AAACATTAAT CACAGATAAC TTGGACCTTA ACTTGAACAA GCAGGCAATC
     2161 AAGTCCAACC AGAAAACTTT GAAGAATGCG CTGGAAATCA AGTCGAAGAT CGACGAGGTG
     2221 CTTACACAGA ACATTATTAG TGAGTATGAC GCACCGGAAC ATATTAAACA AAAGAGCAAA
     2281 ATCTCGCTTA CCACGTTCAA GCTTATCACT AACCTGAAGA AACAGATCAG TTACGTGATC
     2341 ACACGCGTTG AAGAACAAGA GTTGATCGAC AAGTATCAAC AGTGGATCTC CCAGGCTAAG
     2401 CAAGTCGTCC AAGATGAGAA AAATAACTAC GAGCAGATTA TTAAAAAGGC ACCCCGTAAA
     2461 AATCTTGCCG ACTTGGAAAA CTTATTCAAC TTGGAAAAGA GTCTGAAAGA ACAAACAAAT
     2521 TTGAAAATCC TGAACCTTAC AAATACCATG CTTAAAGAGA CTAAGTGAAG AGCTTAGAGA
     2581 CCGGTACTAG TTCCTGCAGG GCTCGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA
     2641 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT
     2701 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA
     2761 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     2821 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     2881 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     2941 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     3001 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     3061 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     3121 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     3181 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
     3241 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     3301 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     3361 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
     3421 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
     3481 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
     3541 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     3601 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     3661 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     3721 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     3781 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     3841 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     3901 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     3961 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     4021 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     4081 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     4141 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     4201 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     4261 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     4321 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     4381 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     4441 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     4501 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     4561 TTGGTCAGGT GGAGTGAATT CGCGGCCGCT TCTAGAGGCT CTTCGTAATT ACGAAGAGGT
     4621 CTCTA
//