LOCUS Exported 4625 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..4625 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 2638..2685 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 4513..4529 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 4530..4561 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 1..0 /note="RnsC: nucleoside ABC transporter, permease protein" /translation="MQSKISWTLSKKSKYLFGSDTTKTKLKYIKGSIFSIIVGFIVSGIFLSFLNINPFTYFALLFGINFDKNFYQISLNWMAVYIVAGLSMAIAFKSGVFNIGASGQILTATSVATIVFFSISGKDASSITPFMIILMLITCIISAAFIAFIAGILKALFNIHEVVSTILLNWSVFYIFKWFFGRYQDFSSGLSYTSKNIPSDQLSIGSNTVIIPLLIALICVIIIWILFSKTVLGFKLKAVGSSITGSKYIGINVKRQIITSLTLSGAVAGIAGFLSMFTVSPNNFFASNSLPTLGFDAIAVSLVAFNNPIGIIAIGWLWAIIKTGGGPISSLYSISTQISGLISGILIYFTAIVSVFIAFKPWELLKNKYNLYTSKVNREIYWKLKLYTFKLRLKKIFLIFTKDYKQEVNNKYQIYTKQNQITNKTSNPHLFWNGRKNIEIELKNDLKQSIYLVKSKIDEIKKFVDQDKNSLNVSGLKNDLNKQVNLLASKYIQNLRDLDLELADHKYKIQKITSSILNEYQTNIKQAKKTHRLKIQQIKVFKESQIGIITYKFDSHNNIIEIKANKLKTIAQLKEQIKNIKSELRLEKQISNLNKSSTQTNNSEKLEKIKQLKEQIKVVRKQANAKILEQKNKYKNQKNSVKQEQVQLQDILHQYNNYLNKEKDRFKESKKAALVLKQKRLQAIDMNLSQSDVNKAVSLLNDLKTLITDNLDLNLNKQAIKSNQKTLKNALEIKSKIDEVLTQNIISEYDAPEHIKQKSKISLTTFKLITNLKKQISYVITRVEEQELIDKYQQWISQAKQVVQDEKNNYEQIIKKAPRKNLADLENLFNLEKSLKEQTNLKILNLTNTMLKETK" /label="MMSYN1_0009" primer_bind complement(2690..2706) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(2773..3361) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(3532..4392) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(4393..4497) /note="ampicillin resistance gene promoter" /label="bla promoter" CDS complement(1..0) /label="MMSYN1_0009" /note="RnsC: nucleoside ABC transporter, permease protein" /translation="MQSKISWTLSKKSKYLFGSDTTKTKLKYIKGSIFSIIVGFIVSGIFLSFLNINPFTYFALLFGINFDKNFYQISLNWMAVYIVAGLSMAIAFKSGVFNIGASGQILTATSVATIVFFSISGKDASSITPFMIILMLITCIISAAFIAFIAGILKALFNIHEVVSTILLNWSVFYIFKWFFGRYQDFSSGLSYTSKNIPSDQLSIGSNTVIIPLLIALICVIIIWILFSKTVLGFKLKAVGSSITGSKYIGINVKRQIITSLTLSGAVAGIAGFLSMFTVSPNNFFASNSLPTLGFDAIAVSLVAFNNPIGIIAIGWLWAIIKTGGGPISSLYSISTQISGLISGILIYFTAIVSVFIAFKPWELLKNKYNLYTSKVNREIYWKLKLYTFKLRLKKIFLIFTKDYKQEVNNKYQIYTKQNQITNKTSNPHLFWNGRKNIEIELKNDLKQSIYLVKSKIDEIKKFVDQDKNSLNVSGLKNDLNKQVNLLASKYIQNLRDLDLELADHKYKIQKITSSILNEYQTNIKQAKKTHRLKIQQIKVFKESQIGIITYKFDSHNNIIEIKANKLKTIAQLKEQIKNIKSELRLEKQISNLNKSSTQTNNSEKLEKIKQLKEQIKVVRKQANAKILEQKNKYKNQKNSVKQEQVQLQDILHQYNNYLNKEKDRFKESKKAALVLKQKRLQAIDMNLSQSDVNKAVSLLNDLKTLITDNLDLNLNKQAIKSNQKTLKNALEIKSKIDEVLTQNIISEYDAPEHIKQKSKISLTTFKLITNLKKQISYVITRVEEQELIDKYQQWISQAKQVVQDEKNNYEQIIKKAPRKNLADLENLFNLEKSLKEQTNLKILNLTNTMLKETK" ORIGIN 1 ATGCAGAGTA AGATTAGTTG GACATTAAGC AAGAAAAGTA AGTATCTTTT CGGCTCTGAC 61 ACCACCAAGA CCAAGTTAAA GTACATTAAG GGCAGCATTT TCTCTATTAT TGTCGGCTTC 121 ATTGTAAGCG GTATCTTCCT GTCCTTCTTG AATATCAATC CTTTTACGTA CTTTGCCCTG 181 TTGTTTGGAA TCAACTTCGA TAAGAATTTT TATCAGATCT CCTTGAACTG GATGGCCGTA 241 TATATCGTGG CAGGCTTAAG TATGGCCATT GCTTTCAAAA GCGGCGTGTT TAACATTGGG 301 GCCTCAGGGC AAATTTTGAC TGCAACATCG GTGGCCACCA TTGTATTCTT TTCCATCAGT 361 GGTAAGGATG CCTCGAGTAT CACACCCTTT ATGATCATCT TAATGTTGAT CACGTGTATT 421 ATCTCTGCTG CGTTTATTGC ATTTATTGCC GGGATTCTTA AGGCACTTTT TAATATCCAC 481 GAGGTCGTAT CGACGATTTT ACTTAATTGG TCCGTATTTT ATATCTTCAA GTGGTTTTTC 541 GGGCGTTACC AGGACTTCTC TTCGGGATTG TCATATACAT CCAAAAACAT CCCATCCGAC 601 CAGCTTTCGA TCGGTTCCAA TACTGTAATT ATTCCGTTAT TGATCGCGTT GATCTGTGTA 661 ATCATTATTT GGATCTTATT TTCAAAGACT GTACTTGGCT TTAAGCTTAA GGCCGTAGGA 721 AGTTCGATTA CTGGTTCGAA GTATATTGGT ATCAACGTAA AACGCCAGAT CATTACAAGC 781 CTTACGCTTT CAGGTGCCGT AGCCGGTATC GCGGGTTTCC TTTCGATGTT CACTGTAAGT 841 CCTAACAACT TCTTCGCCAG CAACTCTTTG CCTACTTTGG GGTTCGATGC CATTGCCGTA 901 TCGTTGGTGG CGTTCAACAA TCCCATCGGG ATCATTGCGA TTGGATGGCT GTGGGCCATC 961 ATTAAGACTG GAGGAGGCCC AATCTCCAGC CTGTACTCGA TCTCGACTCA GATTTCCGGA 1021 TTGATTTCGG GGATCTTAAT TTATTTTACC GCGATTGTCT CAGTCTTTAT TGCCTTCAAG 1081 CCATGGGAAC TTCTTAAGAA TAAATACAAT TTGTACACGT CTAAAGTGAA TCGTGAGATT 1141 TACTGGAAGC TTAAGCTTTA CACATTTAAG TTGCGCCTCA AGAAAATCTT TTTAATCTTT 1201 ACTAAAGACT ATAAGCAGGA GGTAAACAAC AAGTATCAAA TTTACACGAA ACAGAATCAG 1261 ATCACCAACA AGACGAGCAA TCCGCACTTA TTTTGGAATG GACGTAAGAA TATTGAAATC 1321 GAACTGAAAA ATGATCTGAA GCAGTCAATC TATCTTGTCA AGAGCAAGAT TGACGAGATT 1381 AAGAAGTTTG TAGATCAAGA CAAAAATTCG TTGAACGTGT CCGGCTTGAA GAACGACCTG 1441 AACAAGCAAG TGAACTTATT GGCTTCAAAA TACATCCAGA ATTTACGCGA TTTAGACTTA 1501 GAATTAGCCG ATCATAAGTA CAAGATCCAG AAGATCACAT CTAGCATCCT GAATGAGTAC 1561 CAGACAAACA TTAAGCAAGC GAAGAAAACA CACCGCTTGA AGATCCAGCA GATCAAGGTG 1621 TTCAAGGAAT CCCAGATCGG CATTATCACG TATAAATTCG ACTCGCACAA CAACATTATT 1681 GAGATCAAAG CTAATAAGCT GAAAACAATC GCTCAACTGA AGGAACAGAT CAAAAATATC 1741 AAGAGTGAAC TGCGTTTAGA GAAGCAGATT TCTAACCTGA ACAAATCCTC TACCCAGACT 1801 AATAATTCAG AGAAGTTGGA AAAGATTAAA CAATTAAAAG AGCAAATTAA AGTGGTGCGT 1861 AAACAGGCGA ATGCGAAGAT TTTGGAACAG AAAAATAAGT ATAAAAATCA GAAGAATTCG 1921 GTGAAGCAAG AACAGGTCCA ATTACAAGAT ATCTTGCACC AATACAACAA TTACCTTAAT 1981 AAAGAGAAGG ATCGTTTTAA AGAGTCGAAA AAAGCAGCAC TTGTTTTAAA GCAGAAGCGC 2041 CTTCAAGCTA TTGATATGAA TTTAAGTCAA TCCGACGTAA ATAAAGCCGT GAGTCTGCTT 2101 AACGATCTGA AAACATTAAT CACAGATAAC TTGGACCTTA ACTTGAACAA GCAGGCAATC 2161 AAGTCCAACC AGAAAACTTT GAAGAATGCG CTGGAAATCA AGTCGAAGAT CGACGAGGTG 2221 CTTACACAGA ACATTATTAG TGAGTATGAC GCACCGGAAC ATATTAAACA AAAGAGCAAA 2281 ATCTCGCTTA CCACGTTCAA GCTTATCACT AACCTGAAGA AACAGATCAG TTACGTGATC 2341 ACACGCGTTG AAGAACAAGA GTTGATCGAC AAGTATCAAC AGTGGATCTC CCAGGCTAAG 2401 CAAGTCGTCC AAGATGAGAA AAATAACTAC GAGCAGATTA TTAAAAAGGC ACCCCGTAAA 2461 AATCTTGCCG ACTTGGAAAA CTTATTCAAC TTGGAAAAGA GTCTGAAAGA ACAAACAAAT 2521 TTGAAAATCC TGAACCTTAC AAATACCATG CTTAAAGAGA CTAAGTGAAG AGCTTAGAGA 2581 CCGGTACTAG TTCCTGCAGG GCTCGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA 2641 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT 2701 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA 2761 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 2821 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 2881 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 2941 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 3001 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 3061 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 3121 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 3181 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 3241 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 3301 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 3361 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 3421 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 3481 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 3541 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 3601 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 3661 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 3721 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 3781 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 3841 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 3901 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 3961 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 4021 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 4081 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 4141 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 4201 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 4261 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 4321 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 4381 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 4441 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 4501 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 4561 TTGGTCAGGT GGAGTGAATT CGCGGCCGCT TCTAGAGGCT CTTCGTAATT ACGAAGAGGT 4621 CTCTA //