LOCUS       Exported     3704 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3704
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1717..1764
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3592..3608
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3609..3640
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0011"
                     /note="RnsB: nucleoside ABC transporter, solute binding protein"
                     /translation="MKKLLTVLTTFIGISGSVSMVISCKVPTFAEGILGQRVLVVTDGGNIRDKTFNESSWEGVIKYGSQIHSNFDIKNELEARQFNYKSSIGGHTKWDETKHMFINEDYEYAKQNSNNYVETPDHTIDAFRTSYNTAIYKKADALLLAGFGHLGAVDYAADRMKKAGNKTVVFLDAQYKKDNVISVIFNSELAGFNAGWDAIMWANLPKMTSLNSGEFSKEAIDASNSKTDMVLQGSATGNKYISIGMFGGITNKNAVDNYMWGLLAAMHVYNNKFAGKEIELTDNKENKVKYKLQPVYYANLGSKAEVEKLNDVNESSWFSKGFDVGGAKKSGIVDSLIKNQADIIFPVAGPQINDVLESTGHKPYVIGVDTDQVTSVGSSKKGNETRFITSAKKNIVSASVYALNRARSLQKAIIKDKTYTSKHEKEIKDGTTLVGEQADWSISSSRKADTKWSVEKVNGSLTNAANLSVESIDYSKDKAKEIEKNLKETLLKSGETFKQYLTKTSLDKALESISKSIKDEEWEKLTLKDNGIAGIKNYWEMLIQSTKK"
     primer_bind     complement(1769..1785)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1852..2440)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2611..3471)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3472..3576)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     CDS             complement(1..0)
                     /translation="MKKLLTVLTTFIGISGSVSMVISCKVPTFAEGILGQRVLVVTDGGNIRDKTFNESSWEGVIKYGSQIHSNFDIKNELEARQFNYKSSIGGHTKWDETKHMFINEDYEYAKQNSNNYVETPDHTIDAFRTSYNTAIYKKADALLLAGFGHLGAVDYAADRMKKAGNKTVVFLDAQYKKDNVISVIFNSELAGFNAGWDAIMWANLPKMTSLNSGEFSKEAIDASNSKTDMVLQGSATGNKYISIGMFGGITNKNAVDNYMWGLLAAMHVYNNKFAGKEIELTDNKENKVKYKLQPVYYANLGSKAEVEKLNDVNESSWFSKGFDVGGAKKSGIVDSLIKNQADIIFPVAGPQINDVLESTGHKPYVIGVDTDQVTSVGSSKKGNETRFITSAKKNIVSASVYALNRARSLQKAIIKDKTYTSKHEKEIKDGTTLVGEQADWSISSSRKADTKWSVEKVNGSLTNAANLSVESIDYSKDKAKEIEKNLKETLLKSGETFKQYLTKTSLDKALESISKSIKDEEWEKLTLKDNGIAGIKNYWEMLIQSTKK"
                     /label="MMSYN1_0011"
                     /note="RnsB: nucleoside ABC transporter, solute binding protein"
ORIGIN
        1 ATGAAAAAAT TGTTAACTGT ATTAACAACT TTCATCGGGA TCTCCGGGAG TGTTTCTATG
       61 GTGATCAGTT GCAAGGTGCC CACTTTCGCA GAAGGTATCC TGGGTCAACG TGTGTTGGTA
      121 GTAACCGATG GAGGAAATAT TCGCGATAAG ACTTTTAATG AATCGAGTTG GGAAGGCGTG
      181 ATCAAATATG GTAGTCAGAT CCACTCGAAC TTCGATATCA AGAACGAACT GGAGGCCCGT
      241 CAATTCAATT ACAAATCCTC AATCGGGGGG CACACTAAAT GGGATGAGAC TAAACATATG
      301 TTCATCAATG AGGATTATGA ATATGCGAAA CAGAACTCCA ACAACTACGT CGAAACCCCC
      361 GACCATACAA TCGACGCCTT TCGCACATCT TATAACACAG CGATTTATAA GAAGGCTGAC
      421 GCCTTATTAT TGGCCGGATT CGGGCATCTG GGCGCGGTAG ATTATGCGGC TGATCGCATG
      481 AAAAAAGCGG GTAATAAAAC GGTCGTGTTT CTGGACGCAC AGTATAAGAA AGATAACGTG
      541 ATCTCGGTGA TCTTCAACTC GGAACTTGCT GGTTTCAATG CGGGATGGGA TGCGATCATG
      601 TGGGCGAATC TTCCGAAAAT GACGTCCCTT AACTCTGGCG AGTTTTCTAA AGAAGCAATT
      661 GATGCTTCCA ACTCCAAAAC CGATATGGTG TTACAAGGTT CAGCTACGGG TAACAAGTAC
      721 ATCTCTATTG GCATGTTCGG AGGGATCACA AATAAGAACG CCGTCGATAA CTACATGTGG
      781 GGGCTGTTGG CTGCCATGCA TGTCTACAAT AACAAATTCG CGGGGAAAGA AATCGAATTA
      841 ACAGATAACA AGGAAAATAA AGTAAAGTAC AAATTACAGC CCGTCTATTA TGCAAATTTG
      901 GGCTCTAAGG CGGAGGTCGA GAAATTGAAC GACGTGAATG AGTCCTCTTG GTTCTCAAAG
      961 GGGTTCGATG TTGGTGGGGC GAAGAAGTCG GGCATCGTTG ACTCACTGAT TAAAAACCAA
     1021 GCAGATATTA TTTTCCCGGT AGCCGGCCCC CAAATTAACG ACGTCTTAGA GAGTACAGGA
     1081 CACAAACCCT ATGTGATTGG GGTCGATACA GATCAGGTAA CATCGGTCGG AAGTTCTAAA
     1141 AAGGGGAATG AGACTCGTTT TATTACCAGC GCGAAGAAGA ATATAGTCTC GGCATCAGTT
     1201 TATGCGCTGA ACCGCGCCCG CTCATTGCAG AAGGCGATCA TCAAGGACAA AACCTACACC
     1261 TCCAAACACG AAAAGGAGAT CAAAGACGGA ACGACACTGG TCGGGGAGCA AGCTGATTGG
     1321 AGTATCTCCA GCTCGCGCAA GGCTGACACC AAATGGTCCG TTGAAAAGGT CAATGGATCC
     1381 TTAACCAATG CTGCAAACCT GTCGGTCGAG AGCATTGACT ACAGTAAGGA CAAGGCGAAG
     1441 GAGATCGAAA AGAACCTGAA GGAAACCCTG CTGAAGTCGG GTGAAACGTT TAAGCAGTAT
     1501 CTTACTAAAA CAAGCCTGGA TAAAGCTTTA GAAAGCATCT CCAAAAGTAT CAAGGACGAA
     1561 GAATGGGAAA AGCTTACCCT GAAAGATAAC GGTATCGCGG GGATCAAAAA TTACTGGGAA
     1621 ATGCTTATCC AATCTACCAA AAAGTGAAGA GCTTAGAGAC CGGTACTAGT TCCTGCAGGG
     1681 CTCGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT
     1741 AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG
     1801 ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG
     1861 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
     1921 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
     1981 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
     2041 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
     2101 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
     2161 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
     2221 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
     2281 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
     2341 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
     2401 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC
     2461 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT
     2521 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA
     2581 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT
     2641 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC
     2701 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG
     2761 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG
     2821 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT
     2881 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT
     2941 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT
     3001 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT
     3061 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT
     3121 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT
     3181 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC
     3241 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA
     3301 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA
     3361 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA
     3421 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT
     3481 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
     3541 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA
     3601 CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTCAGGTG GAGTGAATTC
     3661 GCGGCCGCTT CTAGAGGCTC TTCGTAATTA CGAAGAGGTC TCTA
//