LOCUS BBF10K_000013 7201 bp ds-DNA circular 18-MAY-2022 DEFINITION . FEATURES Location/Qualifiers source 1..7201 /label="source" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 terminator 38..85 /label="T7 terminator" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer_bind complement(90..106) /label="M13 rev" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 265..515 /label="incC" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac CDS complement(518..1273) /label="repE" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 exon complement(518..1273) /label="Translation 1273-518" rep_origin 1364..1583 /label="ori2" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 protein_bind 2003..2024 /label="CAP binding site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 promoter 2039..2069 /label="lac promoter" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 protein_bind 2077..2093 /label="lac operator" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 primer_bind 2101..2117 /label="M13 rev" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc CDS 2190..3035 /label="repL" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 exon 2190..3035 /label="Translation 2190-3035" misc_feature 3143..3294 /label="nnpp promoters" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 CDS 3334..4149 /label="KanR" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd exon 3334..4149 /label="Translation 3334-4149" terminator complement(4259..4302) /label="bacterial terminator" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer_bind 4320..4336 /label="M13 fwd" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e terminator 4337..4368 /label="tonB terminator" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 4389..7201 /label="gp32" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 ORIGIN 1 cgctgaggtg tcaatcgtcg gagccgctga gcaataacta gcataacccc ttggggcctc 61 taaacgggtc ttgaggggtt ttttgcatgg tcatagctgt ttcctgagag cttggcaggt 121 gtcgcgtatg catcgatcac gtgtccacgt tctttaatag tggactcttg ttccaaactg 181 gaacaacact cggatcgatc cggcgcgcac cgtgggaaaa actccaggta gaggtacaca 241 cgcggatagc caattcagag taataaactg tgataatcaa ccctcatcaa tgatgacgaa 301 ctaacccccg atatcaggtc acatgacgaa gggaaagaga aggaaatcaa ctgtgacaaa 361 ctgccctcaa atttggcttc cttaaaaatt acagttcaaa aagtatgaga aaatccatgc 421 aggctgaagg aaacagcaaa actgtgacaa attaccctca gtaggtcaga acaaatgtga 481 cgaaccaccc tcaaatctgt gacagataac cctcagacta tcctgtcgtc atggaagtga 541 tatcgcggaa ggaaaatacg atatgagtcg tctggcggcc tttctttttc tcaatgtatg 601 agaggcgcat tggagttctg ctgttgatct cattaacaca gacttgcagg aagcggcggc 661 ggaagtcagg catacgctgg taactttgag gcagctggta acgctctatg atccagtcga 721 ttttcagaga cacgatgcct gagccatccg gcttacgata ctgacacagg gattcgtata 781 aacgcatggc atacggattg gtgatttctt ttgtttcact aagccgaaac tgcgtaaacc 841 ggttctgtaa cccgataaag aagggaatga gatatgggtt gatatgtaca ctgtaaagcc 901 ctctggatgg actgtgcgca cgtttgataa accaaggaaa agattcatag cctttttcat 961 caccggcatc ctcttcaggg cgataaaaaa ccacttcctt ccccgcgaaa ctcttcaatg 1021 cctgccgtat atccttactg gcttccgcag aggtcaatcc gaatatttca gcatatttag 1081 caacatggat ctcgcagata ccgtcatgtt cctgtagggt gccatcagat tttctgatct 1141 ggtcaacgaa cagatacagc atacgttttt gatcccggga gagactatat gccgcctcag 1201 tgaggtcgtt tgactggacg attcgcgggc tatttttacg tttcttgtga ttgataaccg 1261 ctgtttccgc catgacagat ccatgtgaag tgtgacaagt ttttagattg tcacactaaa 1321 taaaaaagag tcaataagca gggataactt tgtgaaaaaa cagcttcttc tgagggcaat 1381 ttgtcacagg gttaagggca atttgtcaca gacaggactg tcatttgagg gtgatttgtc 1441 acactgaaag ggcaatttgt cacaacacct tctgtagaac cagcatggat aaaggcctac 1501 aaggcgctct aaaaaagaag atctaaaaac tataaaaaaa ataattataa aaatatcccc 1561 gtggataagt ggataacccc aagggaagtt ttttcaggca tcgtgtgtaa gcagaatata 1621 taagtgctgt tccctggtgc ttcctcgctc actcgaaatt cccggggata gctttatgct 1681 tgtaaaccgt tttgtgaaaa aatttttaaa ataaaaaagg ggacctctag ggtccccaat 1741 taattagtaa tataatctat taaaggtcat tcaaaaggtc atccaccgga tcaattcccc 1801 tgctcgcgca ggctgggtgc caagctctcg ggtaacatca aggcccgatc cttggagccc 1861 ttgccctccc gcacgatgat cgtgccgtga tcgaaatcca gatccttgac ccgcagttgc 1921 aaaccctcac tgatccgatt cattaatgca gctggcacga caggtttccc gactggaaag 1981 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 2041 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 2101 caggaaacag ctatgaccat gattacgcca agcgcggtac ctgtcgcggc aacgcgctaa 2161 cagacgtagt aagaaccacc agcattgtaa tgctggctaa agtcactttc ctgagctgta 2221 taacgatgag cgattttact ttttctggct atgaattggc ctgctttgta acacactccg 2281 gtctatcccg tagcgccggg catatcctgt cgcaatgtgc aaatctcgcg gcaacaacca 2341 gtgaatactt cattcacaag cctcaccgcc tgatcgcggc agaaactggt tatagccaat 2401 caaccgtcgt tcgtgcattc cgtgaagctg taaacaaagg tatcctgtct gtagagattg 2461 ttatcggcga tcaccgtgaa cgtcgcgcta acctgtaccg gtttacacca tcctttttgg 2521 ccttcgcaca acaagccaaa aatgcgctga tagaaagcaa attaaagatc tcttcagcgg 2581 caaccaaggt taaagctgtt ctcgctaaga cattggcttt atttaatttt ttatccacac 2641 ccccatgtca aaatgatacc ccctccccct gtcaggatga cgtggcaata aagaataaga 2701 agtcacaagt taaaaaaaca aaaagatcag tttccggcgg tgccggaaca accagcctca 2761 aaaaattgac ttcatggatc gctaaggcaa aagcaaaggc tgacaatctg cggttatcca 2821 aaaaacgcac tcaaaaacat gagttcaagc agaaagtaga ggcggctgcg cggaaatatg 2881 cttacctgaa gaacaagcgt tcgcctgata ttggcgggat atcaaacttc gataacctac 2941 cgcattgcat gacggtaaac gaagctctta atgcggtttt agccaaaaat aaagataacg 3001 aacaatgggg tataccggca ggattcagag ggtaatgaat tgctctaatt ataaccatgc 3061 atactttcaa cacctctagt ttgccatgag gcaaactcat aggtgtcctg gtaagaggac 3121 actgttgcca aaactggacg ccccattatt gcaattaata aacaactaac ggacaattct 3181 acctaacaat aagtggagtt gcggccggcc gcgatccgct agcaaagcca cgttgtgtct 3241 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg 3301 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct 3361 tgctcgcgcc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct 3421 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg 3481 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg 3541 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt 3601 actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta 3661 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc 3721 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgcctc 3781 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag 3841 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactgtt gccattctca 3901 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg 3961 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt 4021 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa 4081 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag 4141 tttttctaat cagaattggt taattggttg taacactggc gagctctgcc acctgacgtc 4201 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat 4261 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctgcacctg caccagtcag 4321 taaaacgacg gccagtagtc aaaagcctcc gaccggaggc ttttgacttg gttcaggtgg 4381 agtgggagTT AATTAACATT ACTCGCATCC ATTCTCAGGC TGTCTCGTCT CGTCTCACGA 4441 TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG 4501 GTCGAGATCC CGGTGCCTAA TGAGTGAGCT AACTTACATT AATTGCGTTG CGCTCACTGC 4561 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG 4621 GGAGAGGCGG TTTGCGTATT GGGCGCCAGG GTGGTTTTTC TTTTCACCAG TGACACGGGC 4681 AACAGCTGAT TGCCCTTCAC CGCCTGGCCC TGAGAGAGTT GCAGCAAGCG GTCCACGCTG 4741 GTTTGCCCCA GCAGGCGAAA ATCCTGTTTG ATGGTGGTTA ACGGCGGGAT ATAACATGAG 4801 CTATCTTCGG TATCGTCGTA TCCCACTACC GAGATATCCG CACCAACGCG CAGCCCGGAC 4861 TCGGTAATGG CGCGCATTGC GCCCAGCGCC ATCTGATCGT TGGCAACCAG CATCGCAGTG 4921 GGAACGATGC CCTCATTCAG CATTTGCATG GTTTGTTGAA AACCGGACAT GGCACTCCAG 4981 TCGCCTTCCC GTTCCGCTAT CGGCTGAATT TGATTGCGAG TGAGATATTT ATGCCAGCCA 5041 GCCAGACGCA GACGCGCCGA GACAGAACTT AATGGGCCCG CTAACAGCGC GATTTGCTGG 5101 TGACCCAATG CGACCAGATG CTCCACGCCC AGTCGCGTAC CATCTTCATG GGAGAAAATA 5161 ATACTGTTGA TGGGTGTCTG GTCAGAGACA TCAAGAAATA ACGCCGGAAC ATTAGTGCAG 5221 GCAGCTTCCA CAGCAATGGC ATCCTGGTCA TCCAGCGGAT AGTTAATGAT CAGCCCACTG 5281 ACGCGTTGCG CGAGAAGATT GTGCACCGCC GCTTTACAGG CTTCGACGCC GCTTCGTTCT 5341 ACCATCGACA CCACCACGCT GGCACCCAGT TGATCGGCGC GAGATTTAAT CGCCGCGACA 5401 ATTTGCGACG GCGCGTGCAG GGCCAGACTG GAGGTGGCAA CGCCAATCAG CAACGACTGT 5461 TTGCCCGCCA GTTGTTGTGC CACGCGGTTG GGAATGTAAT TCAGCTCCGC CATCGCCGCT 5521 TCCACTTTTT CCCGCGTTTT CGCAGAAACG TGGCTGGCCT GGTTCACCAC GCGGGAAACG 5581 GTCTGATAAG AGACACCGGC ATACTCTGCG ACATCGTATA ACGTTACTGG TTTCACATTC 5641 ACCACCCTGA ATTGACTCTC TTCCGGGCGC TATCATGCCA TACCGCGAAA GGTTTTGCAC 5701 CATTCGATGG TGTCCGGGAT CTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA 5761 GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA 5821 GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC 5881 GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC 5941 GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT 6001 CGAGATCTCG ATCCCGCGAA ATTAATACGA CTCACTATAG GGGAATTGTG AGCGGATAAC 6061 AATTCCCCTC TAGATTTACA CTTTATGCTT CCGGCTCGTA TGTTTCTAGA GAATTCAAAT 6121 AATTTTGTTT AACTTTAAGA AGGAGATATA CCATGCACCA CCACCACCAC CACGGGTCCT 6181 CCGGATTCAA ACGTAAGTCC ACAGCTGAAC TGGCTGCTCA AATGGCAAAG TTAAACGGGA 6241 ATAAAGGATT TTCTTCGGAG GACAAGGGGG AGTGGAAATT GAAGCTGGAT AACGCGGGTA 6301 ATGGGCAAGC GGTCATTCGC TTTCTGCCCT CTAAGAACGA TGAGCAAGCG CCATTCGCAA 6361 TTCTGGTGAA CCATGGGTTC AAAAAAAACG GTAAGTGGTA CATTGAAACG TGCAGTTCAA 6421 CACATGGAGA TTATGATTCC TGTCCCGTAT GCCAGTATAT CTCCAAGAAT GACCTGTACA 6481 ATACCGATAA TAAAGAATAC TCACTGGTCA AACGTAAAAC CAGTTATTGG GCTAACATTC 6541 TGGTAGTTAA AGATCCGGCG GCACCCGAGA ACGAGGGTAA GGTATTCAAG TACCGTTTCG 6601 GTAAAAAGAT TTGGGATAAA ATTAACGCAA TGATCGCCGT AGACGTCGAG ATGGGGGAAA 6661 CCCCCGTTGA TGTTACGTGC CCTTGGGAAG GTGCAAACTT TGTCTTGAAA GTTAAGCAAG 6721 TATCTGGATT TAGTAACTAT GACGAGTCAA AGTTTCTTAA TCAATCAGCG ATTCCAAACA 6781 TTGACGATGA GTCATTCCAA AAAGAACTGT TTGAGCAAAT GGTTGATTTA TCGGAGATGA 6841 CCAGTAAAGA CAAATTTAAG AGTTTCGAGG AACTTAATAC TAAGTTCGGA CAAGTAATGG 6901 GAACCGCCGT AATGGGCGGT GCAGCGGCAA CCGCTGCAAA GAAAGCAGAT AAAGTCGCCG 6961 ACGATTTGGA TGCATTTAAC GTTGACGATT TCAATACGAA AACCGAGGAT GACTTCATGA 7021 GTAGCTCGTC CGGCTCTAGC AGCTCAGCGG ACGATACAGA CTTAGATGAT TTACTGAATG 7081 ATCTGTAATA ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT 7141 GAGGGGTTTT TTGCCCCAGG ATACATAGAT TACCACAACT CCGAGCCCTT CCACCACTAG 7201 T //