LOCUS       BBF10K_000014           8672 bp ds-DNA     circular     18-MAY-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     source          1..8672
                     /label="source"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      38..85
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    265..515
                     /label="incC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(518..1273)
                     /label="repE"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     exon            complement(518..1273)
                     /label="Translation 1273-518"
     rep_origin      1364..1583
                     /label="ori2"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     protein_bind    2003..2024
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        2039..2069
                     /label="lac promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     protein_bind    2077..2093
                     /label="lac operator"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     2101..2117
                     /label="M13 rev"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             2190..3035
                     /label="repL"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     exon            2190..3035
                     /label="Translation 2190-3035"
     misc_feature    3143..3294
                     /label="nnpp promoters"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             3334..4149
                     /label="KanR"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     exon            3334..4149
                     /label="Translation 3334-4149"
     terminator      complement(4259..4302)
                     /label="bacterial terminator"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     4320..4336
                     /label="M13 fwd"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      4337..4368
                     /label="tonB terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    4389..8672
                     /label="E coli optimized RNAse Inhibitor"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 cgctgaggtg tcaatcgtcg gagccgctga gcaataacta gcataacccc ttggggcctc
       61 taaacgggtc ttgaggggtt ttttgcatgg tcatagctgt ttcctgagag cttggcaggt
      121 gtcgcgtatg catcgatcac gtgtccacgt tctttaatag tggactcttg ttccaaactg
      181 gaacaacact cggatcgatc cggcgcgcac cgtgggaaaa actccaggta gaggtacaca
      241 cgcggatagc caattcagag taataaactg tgataatcaa ccctcatcaa tgatgacgaa
      301 ctaacccccg atatcaggtc acatgacgaa gggaaagaga aggaaatcaa ctgtgacaaa
      361 ctgccctcaa atttggcttc cttaaaaatt acagttcaaa aagtatgaga aaatccatgc
      421 aggctgaagg aaacagcaaa actgtgacaa attaccctca gtaggtcaga acaaatgtga
      481 cgaaccaccc tcaaatctgt gacagataac cctcagacta tcctgtcgtc atggaagtga
      541 tatcgcggaa ggaaaatacg atatgagtcg tctggcggcc tttctttttc tcaatgtatg
      601 agaggcgcat tggagttctg ctgttgatct cattaacaca gacttgcagg aagcggcggc
      661 ggaagtcagg catacgctgg taactttgag gcagctggta acgctctatg atccagtcga
      721 ttttcagaga cacgatgcct gagccatccg gcttacgata ctgacacagg gattcgtata
      781 aacgcatggc atacggattg gtgatttctt ttgtttcact aagccgaaac tgcgtaaacc
      841 ggttctgtaa cccgataaag aagggaatga gatatgggtt gatatgtaca ctgtaaagcc
      901 ctctggatgg actgtgcgca cgtttgataa accaaggaaa agattcatag cctttttcat
      961 caccggcatc ctcttcaggg cgataaaaaa ccacttcctt ccccgcgaaa ctcttcaatg
     1021 cctgccgtat atccttactg gcttccgcag aggtcaatcc gaatatttca gcatatttag
     1081 caacatggat ctcgcagata ccgtcatgtt cctgtagggt gccatcagat tttctgatct
     1141 ggtcaacgaa cagatacagc atacgttttt gatcccggga gagactatat gccgcctcag
     1201 tgaggtcgtt tgactggacg attcgcgggc tatttttacg tttcttgtga ttgataaccg
     1261 ctgtttccgc catgacagat ccatgtgaag tgtgacaagt ttttagattg tcacactaaa
     1321 taaaaaagag tcaataagca gggataactt tgtgaaaaaa cagcttcttc tgagggcaat
     1381 ttgtcacagg gttaagggca atttgtcaca gacaggactg tcatttgagg gtgatttgtc
     1441 acactgaaag ggcaatttgt cacaacacct tctgtagaac cagcatggat aaaggcctac
     1501 aaggcgctct aaaaaagaag atctaaaaac tataaaaaaa ataattataa aaatatcccc
     1561 gtggataagt ggataacccc aagggaagtt ttttcaggca tcgtgtgtaa gcagaatata
     1621 taagtgctgt tccctggtgc ttcctcgctc actcgaaatt cccggggata gctttatgct
     1681 tgtaaaccgt tttgtgaaaa aatttttaaa ataaaaaagg ggacctctag ggtccccaat
     1741 taattagtaa tataatctat taaaggtcat tcaaaaggtc atccaccgga tcaattcccc
     1801 tgctcgcgca ggctgggtgc caagctctcg ggtaacatca aggcccgatc cttggagccc
     1861 ttgccctccc gcacgatgat cgtgccgtga tcgaaatcca gatccttgac ccgcagttgc
     1921 aaaccctcac tgatccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     1981 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     2041 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     2101 caggaaacag ctatgaccat gattacgcca agcgcggtac ctgtcgcggc aacgcgctaa
     2161 cagacgtagt aagaaccacc agcattgtaa tgctggctaa agtcactttc ctgagctgta
     2221 taacgatgag cgattttact ttttctggct atgaattggc ctgctttgta acacactccg
     2281 gtctatcccg tagcgccggg catatcctgt cgcaatgtgc aaatctcgcg gcaacaacca
     2341 gtgaatactt cattcacaag cctcaccgcc tgatcgcggc agaaactggt tatagccaat
     2401 caaccgtcgt tcgtgcattc cgtgaagctg taaacaaagg tatcctgtct gtagagattg
     2461 ttatcggcga tcaccgtgaa cgtcgcgcta acctgtaccg gtttacacca tcctttttgg
     2521 ccttcgcaca acaagccaaa aatgcgctga tagaaagcaa attaaagatc tcttcagcgg
     2581 caaccaaggt taaagctgtt ctcgctaaga cattggcttt atttaatttt ttatccacac
     2641 ccccatgtca aaatgatacc ccctccccct gtcaggatga cgtggcaata aagaataaga
     2701 agtcacaagt taaaaaaaca aaaagatcag tttccggcgg tgccggaaca accagcctca
     2761 aaaaattgac ttcatggatc gctaaggcaa aagcaaaggc tgacaatctg cggttatcca
     2821 aaaaacgcac tcaaaaacat gagttcaagc agaaagtaga ggcggctgcg cggaaatatg
     2881 cttacctgaa gaacaagcgt tcgcctgata ttggcgggat atcaaacttc gataacctac
     2941 cgcattgcat gacggtaaac gaagctctta atgcggtttt agccaaaaat aaagataacg
     3001 aacaatgggg tataccggca ggattcagag ggtaatgaat tgctctaatt ataaccatgc
     3061 atactttcaa cacctctagt ttgccatgag gcaaactcat aggtgtcctg gtaagaggac
     3121 actgttgcca aaactggacg ccccattatt gcaattaata aacaactaac ggacaattct
     3181 acctaacaat aagtggagtt gcggccggcc gcgatccgct agcaaagcca cgttgtgtct
     3241 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     3301 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct
     3361 tgctcgcgcc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     3421 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg
     3481 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     3541 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     3601 actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta
     3661 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     3721 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgcctc
     3781 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     3841 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactgtt gccattctca
     3901 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     3961 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     4021 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     4081 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     4141 tttttctaat cagaattggt taattggttg taacactggc gagctctgcc acctgacgtc
     4201 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat
     4261 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctgcacctg caccagtcag
     4321 taaaacgacg gccagtagtc aaaagcctcc gaccggaggc ttttgacttg gttcaggtgg
     4381 agtgggagTT AATTAACATT ACTCGCATCC ATTCTCAGGC TGTCTCGTCT CGTCTCCCGA
     4441 CACCATCGAA TGGTGCAAAA CCTTTCGCGG TATGGCATGA TAGCGCCCGG AAGAGAGTCA
     4501 ATTCAGGGTG GTGAATGTGA AACCAGTAAC GTTATACGAT GTCGCAGAGT ATGCCGGTGT
     4561 CTCTTATCAG ACCGTTTCCC GCGTGGTGAA CCAGGCCAGC CACGTTTCTG CGAAAACGCG
     4621 GGAAAAAGTG GAAGCGGCGA TGGCGGAGCT GAATTACATT CCCAACCGCG TGGCACAACA
     4681 ACTGGCGGGC AAACAGTCGT TGCTGATTGG CGTTGCCACC TCCAGTCTGG CCCTGCACGC
     4741 GCCGTCGCAA ATTGTCGCGG CGATTAAATC TCGCGCCGAT CAACTGGGTG CCAGCGTGGT
     4801 GGTGTCGATG GTAGAACGAA GCGGCGTCGA AGCCTGTAAA GCGGCGGTGC ACAATCTTCT
     4861 CGCGCAACGC GTCAGTGGGC TGATCATTAA CTATCCGCTG GATGACCAGG ATGCCATTGC
     4921 TGTGGAAGCT GCCTGCACTA ATGTTCCGGC GTTATTTCTT GATGTCTCTG ACCAGACACC
     4981 CATCAACAGT ATTATTTTCT CCCATGAAGA TGGTACGCGA CTGGGCGTGG AGCATCTGGT
     5041 CGCATTGGGT CACCAGCAAA TCGCGCTGTT AGCGGGCCCA TTAAGTTCTG TCTCGGCGCG
     5101 TCTGCGTCTG GCTGGCTGGC ATAAATATCT CACTCGCAAT CAAATTCAGC CGATAGCGGA
     5161 ACGGGAAGGC GACTGGAGTG CCATGTCCGG TTTTCAACAA ACCATGCAAA TGCTGAATGA
     5221 GGGCATCGTT CCCACTGCGA TGCTGGTTGC CAACGATCAG ATGGCGCTGG GCGCAATGCG
     5281 CGCCATTACC GAGTCCGGGC TGCGCGTTGG TGCGGATATC TCGGTAGTGG GATACGACGA
     5341 TACCGAAGAT AGCTCATGTT ATATCCCGCC GTTAACCACC ATCAAACAGG ATTTTCGCCT
     5401 GCTGGGGCAA ACCAGCGTGG ACCGCTTGCT GCAACTCTCT CAGGGCCAGG CGGTGAAGGG
     5461 CAATCAGCTG TTGCCCGTGT CACTGGTGAA AAGAAAAACC ACCCTGGCGC CCAATACGCA
     5521 AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG
     5581 ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTAAG TTAGCTCACT CATTAGGCAC
     5641 AATTCTCATG TTTGACAGCT TATCATCGAC TGCACGGTGC ACCAATGCTT CTGGCGTCAG
     5701 GCAGCCATCG GAAGCTGTGG TATGGCTGTG CAGGTCGTAA ATCACTGCAT AATTCGTGTC
     5761 GCTCAAGGCG CACTCCCGTT CTGGATAATG TTTTTTGCGC CGACATCATA ACGGTTCTGG
     5821 CAAATATTCT GAAATGAGCT GTTGACAATT AATCATCGGC TCGTATAATG TGTGGAATTG
     5881 TGAGCGGATA ACAATTTCAC ACAGGAAACA GCCAGTCCGT TTAGGTGTTT TCACGAGCAA
     5941 TTGACCAACA AGGACCATAG ATTATGCACC ACCACCACCA CCACAAAATC GAAGAAGGTA
     6001 AACTGGTAAT CTGGATTAAC GGCGATAAAG GCTATAACGG CCTCGCGGAA GTCGGTAAGA
     6061 AATTCGAGAA AGATACCGGA ATTAAAGTCA CCGTTGAGCA TCCGGATAAA CTGGAAGAGA
     6121 AATTCCCACA GGTTGCGGCA ACTGGCGATG GCCCTGACAT TATCTTCTGG GCACACGACC
     6181 GCTTTGGTGG CTACGCTCAA TCTGGCCTGT TGGCTGAAAT CACCCCGGAC AAAGCGTTCC
     6241 AGGACAAGCT GTATCCGTTT ACCTGGGATG CCGTACGTTA CAACGGCAAG CTGATTGCTT
     6301 ACCCGATCGC TGTTGAAGCG TTATCGCTGA TTTATAACAA AGACCTGCTG CCGAACCCGC
     6361 CAAAAACCTG GGAAGAGATC CCGGCGCTGG ATAAAGAACT GAAAGCGAAA GGTAAGAGCG
     6421 CGCTGATGTT CAACCTGCAA GAACCGTACT TCACCTGGCC GCTGATTGCT GCTGACGGGG
     6481 GTTATGCGTT CAAGTATGAA AACGGCAAGT ACGACATTAA AGACGTGGGC GTGGATAACG
     6541 CTGGCGCGAA AGCGGGTCTG ACCTTCCTGG TTGACCTGAT TAAAAACAAA CACATGAATG
     6601 CAGACACCGA TTACTCCATC GCAGAAGCTG CCTTTAATAA AGGCGAAACA GCGATGACCA
     6661 TCAACGGCCC GTGGGCATGG TCCAACATCG ACACCAGCAA AGTGAATTAT GGTGTAACGG
     6721 TACTGCCGAC CTTCAAGGGT CAACCATCCA AACCGTTCGT TGGCGTGCTG AGCGCAGGTA
     6781 TTAACGCCGC CAGTCCGAAC AAAGAGCTGG CAAAAGAGTT CCTCGAAAAC TATCTGCTGA
     6841 CTGATGAAGG TCTGGAAGCG GTTAATAAAG ACAAACCGCT GGGTGCCGTA GCGCTGAAGT
     6901 CTTACGAGGA AGAGTTGGTG AAAGATCCGC GTATTGCCGC CACTATGGAA AACGCCCAGA
     6961 AAGGTGAAAT CATGCCGAAC ATCCCGCAGA TGTCCGCTTT CTGGTATGCC GTGCGTACTG
     7021 CGGTGATCAA CGCCGCCAGC GGTCGTCAGA CTGTCGATGA AGCCCTGAAA GACGCGCAGA
     7081 CTAATTCGAG CTCGAACAAC AACAACAATA ACAATAACAA CAACCTCGGG ATTGAGGGAA
     7141 GGGAAAATCT TTATTTTCAA GGTAGTCTTG ACATCCAGTG TGAGCAGCTG AGTGATGCCC
     7201 GGTGGACAGA GCTCCTTCCT CTGATCCAGC AATACGAAGT GGTCAGGCTG GATGACTGTG
     7261 GCCTCACTGA AGTGCGGTGC AAAGACATCA GCTCAGCAGT CCAAGCCAAC CCTGCCCTGA
     7321 CAGAGCTCAG CCTTCGCACC AATGAACTGG GTGATGGCGG TGTGGGTCTG GTGCTCCAGG
     7381 GCCTGCAAAA TCCCACTTGT AAGATCCAGA AGCTGAGCCT TCAGAACTGC GGCTTGACGG
     7441 AGGCTGGCTG TGGGATTCTG CCTGGCATGC TGCGCTCTTT GTCTACCCTG CGTGAGCTAC
     7501 ATCTCAATGA TAACCCTATG GGGGACGCAG GCCTGAAGCT GCTCTGTGAA GGACTTCAGG
     7561 ACCCCCAGTG CCGTCTTGAG AAGCTTCAGT TGGAATACTG TAACCTCACA GCTACCAGCT
     7621 GCGAGCCCCT GGCTTCAGTG CTCAGGGTGA AAGCTGACTT TAAGGAGCTA GTATTGAGCA
     7681 ACAACGACCT CCATGAACCT GGTGTCCGTA TTCTGTGCCA GGGCCTGAAG GACTCTGCCT
     7741 GTCAACTGGA GTCACTCAAG CTGGAGAACT GTGGTATCAC AGCAGCCAAC TGCAAGGATC
     7801 TGTGCGATGT TGTGGCCTCC AAAGCTTCGC TACAGGAACT GGACTTGAGC AGCAACAAGC
     7861 TAGGTAATGC AGGCATTGCA GCACTGTGCC CAGGACTGCT GCTTCCCAGC TGTAAGCTCA
     7921 GGACTCTGTG GCTCTGGGAA TGTGATATCA CTGCGGAGGG CTGCAAGGAC CTGTGCCGTG
     7981 TCCTCAGAGC CAAGCAGAGC CTGAAGGAAC TCAGCCTTGC TTCCAATGAG CTGAAGGATG
     8041 AGGGTGCCCG GCTGCTGTGT GAGAGCCTAT TAGAGCCTGG CTGTCAGCTG GAGTCACTGT
     8101 GGATAAAGAC CTGTAGCCTC ACAGCTGCCT CCTGTCCCTA CTTCTGCTCC GTGTTGACCA
     8161 AAAGCCGTTC TCTGTTGGAG CTGCAAATGA GCAGCAACCC ACTGGGAGAT GAGGGAGTCC
     8221 AGGAGCTTTG CAAGGCCCTG AGCCAGCCAG ATACAGTGCT GCGTGAGCTT TGGTTGGGAG
     8281 ACTGTGATGT GACAAATAGT GGTTGCAGCA GCCTTGCCAA TGTCCTGCTG GCCAACCGCA
     8341 GCCTGAGGGA ACTGGACCTC AGTAACAACT GCATGGGGGG CCCAGGTGTC CTGCAACTGC
     8401 TGGAGAGCCT CAAACAGCCC AGCTGCACCC TTCAGCAGCT CGTCCTGTAT GACATTTACT
     8461 GGACAAATGA GGTGGAAGAA CAGCTTCGGG CCCTGGAGGA GGAAAGGCCT TCCCTGAGGA
     8521 TCATTTCCTA ACCTGCACAA ATAAAACGAA AGGCTCAGTC GAAAGACTGG GCCTTTCGTT
     8581 TTATCTGTTG TTTGTCGGTG AACGCTCTCC TGAGTAGGAC AAATCCCCAG GATACATAGA
     8641 TTACCACAAC TCCGAGCCCT TCCACCACTA GT
//