LOCUS       Exported     5182 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..5182
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             2010..5168
                     /label="MMSYN1_0033"
                     /note="hypothetical protein"
                     /translation="MLKFIKNNKWWVAIISVFAIFLSSFGIFAKSYVDSNKQKIVNKVQNYVQASSYAVQSRILKETENLNEDYLNQKIGKKSLLDEFSNDFIWRPNNTKTTSTDTISDLWNTYFGSSTNVLDKNLQIQYKNNNEYKNIENSKGEITPQNIDFLFSISKSLEKFLNGFAPSLASLGLSFIQNTVLNNREKSNFKNYKDGLNKFADIIENNKNLFSYLGKILTPKQLEKDYYNNLTVQQALIKNINQIAAAISNDQEFSKEVETDKIPEALDKLLTELGLDSLSEIIGELINSQNGSTNLTQLFNKIKNIFTLKNFEKLKAKALELLDRITPHLATYLYSEIFFGLYYAANQHIKDPNELLVQKVDSNKFLALTNNKLDLGILLNGIEVILKDKKGFERFYNFIFKRFDENKIFNNLNNISSNKGTGNLTYDLLNWLEDKLNGFSNVLNILIKFAEIALNDSNIIKTIQEKIVSFIKEKLPKISSGDWKVEFKFESIEISLSFLGIRTPLYLKANLFGKAGLLSQVINILKSLNNFVDYLSNWFFKYIKNTFYLKSSEKLSVVLLQKLINDIDVLLKDNKNIYITIAQDVISVWPFGKPDVEIKTIYDFLTLPYNKEFLNGLVYKRAEKDIKPAVEKLKTFLESLKTYNFITESTKLKEQFPQYLENLSKYIKKYEEIEITDFNLLNSLYEGNIISDFALKWIEFLTKDISKEDNPVLPILRTIFKDEKFEKLGQIKNKWTTKISELANKIKEFENITKIKNIKINLPEDLLKQFGLESLNTQTIYQLIQTLTTYFNDYLSINPNKVIGLNISSIGKILTALTIKVSVEYNTRNKDKNFLYNKDPLKDKSKTLLKALAYGFDTHDNYSDNIVNISNIRPSESYYNWDKIDFYINGSDKPFTINRTNLKEEQSYSPLHILLGIDVDKTSYIKDSLGYVFGTLFGGLSASDPNYKLSIENKTDATSILNVFNYVLDKKDKQLKKQEDQIATQYYDKTAWSTKILNSSENEINYQLIRLKTSNTDKSKQLGTKFEVKLLKNKNNSYWTINKIIALDYKTA"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGTTAAAATT TATTAAAAAT AACAAGTGGT
     2041 GGGTAGCGAT TATTTCAGTA TTTGCCATTT TCCTTTCCAG CTTTGGCATC TTTGCAAAGT
     2101 CTTACGTAGA TTCTAATAAA CAAAAAATCG TTAACAAGGT GCAAAATTAT GTTCAAGCAT
     2161 CCAGCTACGC CGTTCAGAGT CGCATTCTGA AAGAGACAGA GAATCTGAAT GAAGATTACT
     2221 TAAACCAGAA GATTGGGAAA AAGAGCCTTC TGGATGAGTT CTCCAATGAT TTCATCTGGC
     2281 GCCCCAATAA CACCAAGACC ACCTCAACAG ACACCATCTC TGATTTATGG AACACGTACT
     2341 TTGGAAGCTC GACAAACGTC CTGGACAAGA ATTTACAGAT TCAATATAAG AATAACAACG
     2401 AGTACAAAAA CATTGAGAAC AGCAAAGGAG AGATTACCCC GCAGAACATC GATTTTCTGT
     2461 TTAGCATTTC AAAATCGTTG GAGAAGTTCT TGAACGGGTT TGCTCCTTCG CTTGCGTCCT
     2521 TGGGACTTTC GTTTATCCAG AACACCGTTT TGAATAATCG TGAAAAGAGT AACTTTAAGA
     2581 ACTACAAAGA TGGGTTGAAC AAATTTGCAG ATATTATTGA AAATAACAAA AACTTATTTA
     2641 GCTATTTGGG AAAAATTTTG ACACCCAAAC AACTGGAGAA AGACTACTAT AACAACTTAA
     2701 CCGTGCAGCA GGCGTTGATT AAAAACATCA ACCAAATCGC AGCTGCGATT TCTAACGACC
     2761 AGGAATTTAG CAAGGAAGTT GAGACTGACA AAATCCCAGA GGCCCTTGAT AAATTACTTA
     2821 CAGAATTGGG GTTGGATTCA CTTTCAGAGA TCATCGGTGA GTTAATCAAT AGCCAAAACG
     2881 GCTCAACGAA TTTGACTCAA CTTTTTAATA AAATCAAAAA CATCTTCACT TTAAAAAATT
     2941 TCGAGAAACT GAAGGCAAAG GCTTTAGAGC TTCTTGACCG CATCACGCCC CATCTGGCGA
     3001 CCTACTTATA CTCAGAGATC TTTTTCGGAC TGTACTACGC TGCCAACCAA CATATCAAAG
     3061 ATCCGAACGA GCTTCTGGTT CAAAAGGTCG ACAGTAACAA ATTTCTGGCC CTTACTAACA
     3121 ACAAACTGGA CTTAGGTATT TTGCTTAACG GTATCGAAGT AATCCTGAAA GATAAGAAAG
     3181 GTTTTGAACG CTTTTATAAC TTTATTTTTA AACGCTTCGA TGAAAATAAG ATCTTTAACA
     3241 ATCTTAACAA CATTAGCAGC AACAAAGGTA CAGGGAACTT AACATACGAT CTTTTGAATT
     3301 GGCTTGAGGA CAAATTAAAC GGCTTCTCAA ACGTCCTGAA CATTTTAATT AAATTTGCGG
     3361 AAATTGCTCT GAATGATTCT AACATCATTA AGACAATTCA GGAAAAGATC GTTAGCTTCA
     3421 TCAAAGAAAA ACTTCCTAAG ATCTCCAGTG GAGACTGGAA GGTCGAATTT AAGTTTGAGA
     3481 GTATCGAAAT CTCTTTATCA TTCCTTGGTA TCCGTACCCC TCTGTATTTG AAGGCGAACT
     3541 TGTTCGGTAA AGCAGGGCTT TTATCTCAGG TAATCAACAT TCTGAAATCT TTGAACAATT
     3601 TCGTGGATTA TCTTTCTAAT TGGTTCTTTA AATATATCAA GAACACTTTT TATCTGAAGT
     3661 CAAGCGAGAA GTTGTCTGTT GTACTGCTTC AAAAATTAAT CAACGACATC GACGTGCTTC
     3721 TGAAAGACAA CAAAAACATT TACATTACTA TCGCCCAGGA TGTGATCTCG GTTTGGCCTT
     3781 TTGGGAAGCC AGACGTTGAA ATTAAAACTA TCTATGATTT TCTTACGCTG CCTTACAACA
     3841 AGGAGTTCTT GAACGGCTTG GTATACAAGC GCGCCGAAAA GGATATTAAG CCTGCAGTAG
     3901 AAAAGCTGAA AACTTTTCTT GAAAGTCTTA AGACATACAA CTTCATCACC GAGTCAACTA
     3961 AGCTGAAGGA GCAGTTTCCT CAATACTTGG AGAATCTTTC TAAATACATT AAAAAGTACG
     4021 AAGAAATTGA GATTACGGAC TTCAACTTGC TGAATTCTCT TTATGAAGGC AACATTATTT
     4081 CCGATTTTGC GCTGAAGTGG ATTGAATTTT TAACAAAAGA CATTTCGAAG GAGGACAATC
     4141 CAGTCTTGCC TATTCTGCGC ACAATCTTTA AAGATGAAAA GTTCGAGAAA TTAGGACAAA
     4201 TTAAGAACAA ATGGACGACT AAAATCTCTG AATTAGCAAA CAAGATTAAG GAGTTCGAAA
     4261 ACATTACGAA AATCAAAAAT ATCAAGATTA ATTTGCCTGA AGATCTGCTG AAACAATTCG
     4321 GTCTGGAATC CCTTAACACG CAGACAATTT ATCAATTGAT TCAGACGTTA ACCACTTATT
     4381 TCAATGATTA TCTTTCTATC AATCCAAACA AAGTTATCGG GTTGAACATC TCATCAATTG
     4441 GGAAAATCTT AACCGCCCTT ACCATCAAAG TATCCGTAGA GTACAATACT CGTAATAAAG
     4501 ATAAGAATTT CCTTTACAAT AAGGACCCCT TAAAGGACAA ATCTAAGACG CTGCTGAAGG
     4561 CACTTGCATA TGGTTTCGAT ACCCACGATA ATTACTCAGA TAACATTGTA AACATTTCAA
     4621 ATATTCGCCC GTCCGAATCG TACTATAACT GGGACAAGAT TGATTTCTAT ATTAACGGTT
     4681 CAGACAAGCC ATTTACGATT AACCGCACCA ATTTAAAGGA GGAACAATCA TATTCACCTT
     4741 TACATATCTT ACTGGGCATT GATGTAGATA AGACTAGCTA TATCAAGGAT TCCCTGGGTT
     4801 ACGTATTCGG CACTCTTTTC GGTGGCCTTT CGGCTTCCGA CCCGAATTAT AAATTATCTA
     4861 TTGAAAACAA GACGGATGCG ACTTCTATTC TGAATGTGTT CAATTACGTA TTAGACAAAA
     4921 AAGACAAGCA ACTGAAAAAG CAGGAGGACC AAATTGCCAC GCAGTATTAT GACAAGACTG
     4981 CATGGAGTAC AAAGATCTTG AACTCTTCAG AAAATGAGAT TAACTACCAG TTGATCCGCC
     5041 TTAAGACTTC TAACACCGAT AAGTCCAAGC AGCTTGGTAC CAAGTTCGAA GTAAAATTAT
     5101 TAAAGAATAA GAATAACTCA TATTGGACGA TCAATAAAAT TATCGCTCTT GACTACAAGA
     5161 CTGCCTGAAG AGCTTAGAGA CC
//