LOCUS       Exported     4004 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4004
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2017..2064
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     3892..3908
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3909..3940
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0039"
                     /note="Putative FtsH peptidase"
                     /translation="MNKKKKKSTFWFWIILIVGFIILLSVISITSRGTTQNLTIEQLNSLFDQGKPFNNVVLQRNNIQGIDIITGWYNNGSGWTKFTVNTNPNAINGFSDAFKNFVWRSNTTRYTESSWFSLLSSLLPMLILILFYIGLFYFMAKGGAAGAGANGLFGMGKNKARREKSNVKFSDVAGIEEEKSELVELVDYLKQPAKYASAGARAPKGVLMEGPPGTGKTLLAKAVAGEANVSFFSIAGSEFEEMFVGVGASRVREMFNEAKKAAPAIIFIDEIDAVGRKRNSAIGTGTNEQTLNQLLVELDGFETNSGIIVMAATNRVDVLDPALLRPGRFDRVIQVSLPDIKEREQILKLHARNKKIDPSIDWHRIAERTPGFSGAQLENVLNEAAILMVREGKTVIGINEIDEAIDRVVGGPAKKSRAMTMHDKEIVSYHESGHALIGLKLESASKVQKVTIIPRGNAGGYTIMTPKDETLFSSKTDLYAMIAGYLGGRAAEEIKFGKDNVTTGAHDDFDKATAIARRMVMQFGMSELGITKFLTMADEAYGKTEGSYSEKTAAKIDAEVERILEESYKLAIKVISENMETLELLAESLRVLETITAEQIDYINKNKKLPEAVIYEKEKYKQEQEKINSGKIIDLDINDVKEEEDKDK"
     primer_bind     complement(2069..2085)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2152..2740)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2911..3771)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3772..3876)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0039"
                     /note="Putative FtsH peptidase"
                     /translation="MNKKKKKSTFWFWIILIVGFIILLSVISITSRGTTQNLTIEQLNSLFDQGKPFNNVVLQRNNIQGIDIITGWYNNGSGWTKFTVNTNPNAINGFSDAFKNFVWRSNTTRYTESSWFSLLSSLLPMLILILFYIGLFYFMAKGGAAGAGANGLFGMGKNKARREKSNVKFSDVAGIEEEKSELVELVDYLKQPAKYASAGARAPKGVLMEGPPGTGKTLLAKAVAGEANVSFFSIAGSEFEEMFVGVGASRVREMFNEAKKAAPAIIFIDEIDAVGRKRNSAIGTGTNEQTLNQLLVELDGFETNSGIIVMAATNRVDVLDPALLRPGRFDRVIQVSLPDIKEREQILKLHARNKKIDPSIDWHRIAERTPGFSGAQLENVLNEAAILMVREGKTVIGINEIDEAIDRVVGGPAKKSRAMTMHDKEIVSYHESGHALIGLKLESASKVQKVTIIPRGNAGGYTIMTPKDETLFSSKTDLYAMIAGYLGGRAAEEIKFGKDNVTTGAHDDFDKATAIARRMVMQFGMSELGITKFLTMADEAYGKTEGSYSEKTAAKIDAEVERILEESYKLAIKVISENMETLELLAESLRVLETITAEQIDYINKNKKLPEAVIYEKEKYKQEQEKINSGKIIDLDINDVKEEEDKDK"
ORIGIN
        1 ATGAACAAGA AGAAAAAGAA GTCAACCTTC TGGTTCTGGA TTATTCTGAT CGTTGGATTC
       61 ATCATTTTGT TAAGCGTTAT TAGTATTACC TCGCGCGGCA CCACACAGAA CCTGACCATT
      121 GAGCAATTGA ATAGCCTTTT CGATCAAGGG AAGCCGTTTA ACAACGTAGT CCTGCAGCGC
      181 AATAACATTC AGGGTATTGA CATCATTACT GGGTGGTATA ATAACGGCTC AGGATGGACC
      241 AAGTTTACAG TTAACACCAA CCCCAATGCT ATCAACGGCT TTTCGGATGC ATTCAAGAAT
      301 TTTGTCTGGC GCTCCAACAC AACTCGTTAT ACTGAATCTT CTTGGTTCTC ACTTCTTTCA
      361 TCGTTGCTGC CAATGTTGAT CCTGATTCTC TTTTACATTG GACTGTTTTA CTTCATGGCG
      421 AAAGGAGGCG CTGCGGGGGC CGGCGCAAAT GGCCTTTTCG GCATGGGGAA GAATAAAGCG
      481 CGTCGTGAAA AATCCAATGT CAAATTCTCA GATGTCGCCG GGATTGAGGA AGAAAAATCT
      541 GAATTAGTTG AGCTTGTCGA TTATTTAAAA CAGCCAGCCA AATATGCGTC AGCGGGGGCC
      601 CGCGCCCCTA AAGGTGTTTT GATGGAGGGA CCTCCTGGTA CTGGGAAAAC GTTATTAGCC
      661 AAGGCAGTCG CTGGCGAGGC CAATGTCTCG TTCTTTTCGA TTGCAGGCAG CGAATTTGAA
      721 GAAATGTTTG TCGGGGTAGG CGCTAGTCGC GTACGCGAGA TGTTCAATGA GGCCAAAAAG
      781 GCGGCACCCG CCATCATCTT TATTGACGAG ATTGATGCTG TAGGTCGTAA ACGCAACAGC
      841 GCCATTGGTA CTGGTACCAA TGAGCAGACC TTGAATCAAT TATTAGTTGA GCTTGATGGG
      901 TTCGAGACAA ATTCTGGTAT TATCGTTATG GCCGCAACGA ACCGTGTCGA TGTTCTGGAT
      961 CCGGCCTTAC TTCGCCCAGG TCGTTTTGAC CGCGTGATCC AGGTTTCTTT ACCTGACATC
     1021 AAGGAGCGCG AACAAATCTT AAAGCTTCAC GCGCGCAATA AGAAGATTGA TCCGTCTATT
     1081 GATTGGCACC GGATCGCGGA GCGCACTCCC GGCTTCAGTG GCGCACAGCT TGAAAACGTG
     1141 TTAAATGAGG CTGCAATCCT GATGGTTCGC GAAGGTAAGA CTGTAATTGG TATTAACGAA
     1201 ATTGATGAAG CGATCGATCG TGTAGTGGGA GGTCCTGCTA AGAAGTCACG TGCAATGACC
     1261 ATGCATGACA AGGAAATCGT TTCCTATCAT GAGTCCGGGC ACGCTCTGAT CGGACTGAAA
     1321 CTTGAATCGG CATCTAAAGT TCAGAAGGTT ACGATCATTC CGCGCGGCAA CGCAGGAGGA
     1381 TACACAATCA TGACTCCTAA GGATGAGACT CTTTTTTCAA GCAAAACTGA CCTGTACGCC
     1441 ATGATTGCTG GATACCTGGG AGGACGCGCT GCGGAGGAAA TTAAGTTCGG GAAAGATAAC
     1501 GTCACCACGG GTGCACATGA TGATTTCGAC AAAGCTACAG CGATTGCACG CCGTATGGTT
     1561 ATGCAATTTG GGATGTCCGA ACTTGGAATC ACAAAGTTTT TAACAATGGC TGACGAAGCA
     1621 TACGGCAAAA CGGAGGGCAG CTACAGCGAA AAGACAGCGG CAAAGATCGA TGCCGAGGTC
     1681 GAGCGCATCC TGGAAGAGTC TTATAAACTG GCAATTAAGG TCATTAGTGA GAATATGGAA
     1741 ACATTAGAGT TGTTGGCTGA ATCTCTTCGC GTGTTAGAGA CTATCACAGC AGAGCAGATT
     1801 GATTACATCA ATAAAAACAA AAAGCTGCCC GAGGCCGTGA TTTACGAAAA AGAAAAGTAT
     1861 AAACAGGAGC AGGAAAAAAT CAACAGTGGA AAAATTATCG ATCTGGATAT CAATGATGTT
     1921 AAGGAGGAGG AGGATAAGGA TAAGTGAAGA GCTTAGAGAC CGGTACTAGT TCCTGCAGGG
     1981 CTCGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT
     2041 AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG
     2101 ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG
     2161 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
     2221 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
     2281 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
     2341 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
     2401 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
     2461 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
     2521 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
     2581 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
     2641 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
     2701 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC
     2761 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT
     2821 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA
     2881 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT
     2941 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC
     3001 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG
     3061 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG
     3121 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT
     3181 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT
     3241 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT
     3301 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT
     3361 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT
     3421 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT
     3481 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC
     3541 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA
     3601 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA
     3661 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA
     3721 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT
     3781 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
     3841 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA
     3901 CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTCAGGTG GAGTGAATTC
     3961 GCGGCCGCTT CTAGAGGCTC TTCGTAATTA CGAAGAGGTC TCTA
//