LOCUS Exported 4004 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..4004 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 2017..2064 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" primer_bind 3892..3908 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3909..3940 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 1..0 /label="MMSYN1_0039" /note="Putative FtsH peptidase" /translation="MNKKKKKSTFWFWIILIVGFIILLSVISITSRGTTQNLTIEQLNSLFDQGKPFNNVVLQRNNIQGIDIITGWYNNGSGWTKFTVNTNPNAINGFSDAFKNFVWRSNTTRYTESSWFSLLSSLLPMLILILFYIGLFYFMAKGGAAGAGANGLFGMGKNKARREKSNVKFSDVAGIEEEKSELVELVDYLKQPAKYASAGARAPKGVLMEGPPGTGKTLLAKAVAGEANVSFFSIAGSEFEEMFVGVGASRVREMFNEAKKAAPAIIFIDEIDAVGRKRNSAIGTGTNEQTLNQLLVELDGFETNSGIIVMAATNRVDVLDPALLRPGRFDRVIQVSLPDIKEREQILKLHARNKKIDPSIDWHRIAERTPGFSGAQLENVLNEAAILMVREGKTVIGINEIDEAIDRVVGGPAKKSRAMTMHDKEIVSYHESGHALIGLKLESASKVQKVTIIPRGNAGGYTIMTPKDETLFSSKTDLYAMIAGYLGGRAAEEIKFGKDNVTTGAHDDFDKATAIARRMVMQFGMSELGITKFLTMADEAYGKTEGSYSEKTAAKIDAEVERILEESYKLAIKVISENMETLELLAESLRVLETITAEQIDYINKNKKLPEAVIYEKEKYKQEQEKINSGKIIDLDINDVKEEEDKDK" primer_bind complement(2069..2085) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(2152..2740) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2911..3771) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(3772..3876) /label="bla promoter" /note="ampicillin resistance gene promoter" CDS complement(1..0) /label="MMSYN1_0039" /note="Putative FtsH peptidase" /translation="MNKKKKKSTFWFWIILIVGFIILLSVISITSRGTTQNLTIEQLNSLFDQGKPFNNVVLQRNNIQGIDIITGWYNNGSGWTKFTVNTNPNAINGFSDAFKNFVWRSNTTRYTESSWFSLLSSLLPMLILILFYIGLFYFMAKGGAAGAGANGLFGMGKNKARREKSNVKFSDVAGIEEEKSELVELVDYLKQPAKYASAGARAPKGVLMEGPPGTGKTLLAKAVAGEANVSFFSIAGSEFEEMFVGVGASRVREMFNEAKKAAPAIIFIDEIDAVGRKRNSAIGTGTNEQTLNQLLVELDGFETNSGIIVMAATNRVDVLDPALLRPGRFDRVIQVSLPDIKEREQILKLHARNKKIDPSIDWHRIAERTPGFSGAQLENVLNEAAILMVREGKTVIGINEIDEAIDRVVGGPAKKSRAMTMHDKEIVSYHESGHALIGLKLESASKVQKVTIIPRGNAGGYTIMTPKDETLFSSKTDLYAMIAGYLGGRAAEEIKFGKDNVTTGAHDDFDKATAIARRMVMQFGMSELGITKFLTMADEAYGKTEGSYSEKTAAKIDAEVERILEESYKLAIKVISENMETLELLAESLRVLETITAEQIDYINKNKKLPEAVIYEKEKYKQEQEKINSGKIIDLDINDVKEEEDKDK" ORIGIN 1 ATGAACAAGA AGAAAAAGAA GTCAACCTTC TGGTTCTGGA TTATTCTGAT CGTTGGATTC 61 ATCATTTTGT TAAGCGTTAT TAGTATTACC TCGCGCGGCA CCACACAGAA CCTGACCATT 121 GAGCAATTGA ATAGCCTTTT CGATCAAGGG AAGCCGTTTA ACAACGTAGT CCTGCAGCGC 181 AATAACATTC AGGGTATTGA CATCATTACT GGGTGGTATA ATAACGGCTC AGGATGGACC 241 AAGTTTACAG TTAACACCAA CCCCAATGCT ATCAACGGCT TTTCGGATGC ATTCAAGAAT 301 TTTGTCTGGC GCTCCAACAC AACTCGTTAT ACTGAATCTT CTTGGTTCTC ACTTCTTTCA 361 TCGTTGCTGC CAATGTTGAT CCTGATTCTC TTTTACATTG GACTGTTTTA CTTCATGGCG 421 AAAGGAGGCG CTGCGGGGGC CGGCGCAAAT GGCCTTTTCG GCATGGGGAA GAATAAAGCG 481 CGTCGTGAAA AATCCAATGT CAAATTCTCA GATGTCGCCG GGATTGAGGA AGAAAAATCT 541 GAATTAGTTG AGCTTGTCGA TTATTTAAAA CAGCCAGCCA AATATGCGTC AGCGGGGGCC 601 CGCGCCCCTA AAGGTGTTTT GATGGAGGGA CCTCCTGGTA CTGGGAAAAC GTTATTAGCC 661 AAGGCAGTCG CTGGCGAGGC CAATGTCTCG TTCTTTTCGA TTGCAGGCAG CGAATTTGAA 721 GAAATGTTTG TCGGGGTAGG CGCTAGTCGC GTACGCGAGA TGTTCAATGA GGCCAAAAAG 781 GCGGCACCCG CCATCATCTT TATTGACGAG ATTGATGCTG TAGGTCGTAA ACGCAACAGC 841 GCCATTGGTA CTGGTACCAA TGAGCAGACC TTGAATCAAT TATTAGTTGA GCTTGATGGG 901 TTCGAGACAA ATTCTGGTAT TATCGTTATG GCCGCAACGA ACCGTGTCGA TGTTCTGGAT 961 CCGGCCTTAC TTCGCCCAGG TCGTTTTGAC CGCGTGATCC AGGTTTCTTT ACCTGACATC 1021 AAGGAGCGCG AACAAATCTT AAAGCTTCAC GCGCGCAATA AGAAGATTGA TCCGTCTATT 1081 GATTGGCACC GGATCGCGGA GCGCACTCCC GGCTTCAGTG GCGCACAGCT TGAAAACGTG 1141 TTAAATGAGG CTGCAATCCT GATGGTTCGC GAAGGTAAGA CTGTAATTGG TATTAACGAA 1201 ATTGATGAAG CGATCGATCG TGTAGTGGGA GGTCCTGCTA AGAAGTCACG TGCAATGACC 1261 ATGCATGACA AGGAAATCGT TTCCTATCAT GAGTCCGGGC ACGCTCTGAT CGGACTGAAA 1321 CTTGAATCGG CATCTAAAGT TCAGAAGGTT ACGATCATTC CGCGCGGCAA CGCAGGAGGA 1381 TACACAATCA TGACTCCTAA GGATGAGACT CTTTTTTCAA GCAAAACTGA CCTGTACGCC 1441 ATGATTGCTG GATACCTGGG AGGACGCGCT GCGGAGGAAA TTAAGTTCGG GAAAGATAAC 1501 GTCACCACGG GTGCACATGA TGATTTCGAC AAAGCTACAG CGATTGCACG CCGTATGGTT 1561 ATGCAATTTG GGATGTCCGA ACTTGGAATC ACAAAGTTTT TAACAATGGC TGACGAAGCA 1621 TACGGCAAAA CGGAGGGCAG CTACAGCGAA AAGACAGCGG CAAAGATCGA TGCCGAGGTC 1681 GAGCGCATCC TGGAAGAGTC TTATAAACTG GCAATTAAGG TCATTAGTGA GAATATGGAA 1741 ACATTAGAGT TGTTGGCTGA ATCTCTTCGC GTGTTAGAGA CTATCACAGC AGAGCAGATT 1801 GATTACATCA ATAAAAACAA AAAGCTGCCC GAGGCCGTGA TTTACGAAAA AGAAAAGTAT 1861 AAACAGGAGC AGGAAAAAAT CAACAGTGGA AAAATTATCG ATCTGGATAT CAATGATGTT 1921 AAGGAGGAGG AGGATAAGGA TAAGTGAAGA GCTTAGAGAC CGGTACTAGT TCCTGCAGGG 1981 CTCGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT 2041 AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG 2101 ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG 2161 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 2221 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 2281 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 2341 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 2401 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 2461 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 2521 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 2581 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 2641 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 2701 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 2761 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 2821 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 2881 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT 2941 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC 3001 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG 3061 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG 3121 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT 3181 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT 3241 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT 3301 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT 3361 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT 3421 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT 3481 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC 3541 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA 3601 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA 3661 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA 3721 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT 3781 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA 3841 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA 3901 CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTCAGGTG GAGTGAATTC 3961 GCGGCCGCTT CTAGAGGCTC TTCGTAATTA CGAAGAGGTC TCTA //