LOCUS       Exported     3263 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3263
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1276..1323
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3151..3167
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3168..3199
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0040"
                     /note="TilS: tRNA(Ile)-lysidine synthase"
                     /translation="MKLFNINKSKKYLIAVSGGPDSVFLLCNVVKLVDPNNLVVCHVNYNFRSDSNNDQMIVTNLCKQFNLKLEILNINKDYSLLKQNFESWARVQRYDFFNMIASKYQIYNLLVAHNFNDLIETYLLQLQRNNLVDYYGLKPVSHYKDLVVYRPLLDIKKSQILNYLNTNKISYAIDSTNSDTKYQRNKIRTTLNENNFTKILDQINKANNNLNSIKKIVDNYLKDNIINNELNLTKELFLFDQNSIQRIIYTYFKLINKESLLLNRSNKTIIEIVKRLVNSNKNHWKINLNDHSLIKDYNKLFVIKNSLLEPKTIIINDLNDLINQTTFKNIKEIEQIILKDKNFSYVITNNYEMYKSITTIANKKTNRYFIDRKISYKTRLLSPVVYNVKDKVILNKIKKHY"
     primer_bind     complement(1328..1344)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1411..1999)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2170..3030)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3031..3135)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0040"
                     /note="TilS: tRNA(Ile)-lysidine synthase"
                     /translation="MKLFNINKSKKYLIAVSGGPDSVFLLCNVVKLVDPNNLVVCHVNYNFRSDSNNDQMIVTNLCKQFNLKLEILNINKDYSLLKQNFESWARVQRYDFFNMIASKYQIYNLLVAHNFNDLIETYLLQLQRNNLVDYYGLKPVSHYKDLVVYRPLLDIKKSQILNYLNTNKISYAIDSTNSDTKYQRNKIRTTLNENNFTKILDQINKANNNLNSIKKIVDNYLKDNIINNELNLTKELFLFDQNSIQRIIYTYFKLINKESLLLNRSNKTIIEIVKRLVNSNKNHWKINLNDHSLIKDYNKLFVIKNSLLEPKTIIINDLNDLINQTTFKNIKEIEQIILKDKNFSYVITNNYEMYKSITTIANKKTNRYFIDRKISYKTRLLSPVVYNVKDKVILNKIKKHY"
ORIGIN
        1 ATGAAGTTAT TTAACATTAA CAAGTCCAAG AAATACCTGA TCGCAGTTTC TGGAGGGCCG
       61 GACTCCGTTT TCCTGCTGTG CAACGTGGTC AAGTTAGTAG ATCCTAATAA TTTAGTAGTG
      121 TGCCACGTGA ACTACAACTT CCGCTCCGAC AGCAATAATG ACCAGATGAT TGTCACCAAC
      181 CTGTGTAAGC AGTTCAATTT GAAGTTGGAG ATCTTAAATA TCAATAAGGA CTATTCGCTT
      241 CTGAAACAAA ACTTCGAATC ATGGGCGCGT GTCCAGCGCT ACGACTTTTT TAATATGATT
      301 GCAAGCAAGT ACCAGATCTA TAACTTGTTG GTCGCACATA ACTTTAATGA TCTGATCGAA
      361 ACCTATTTGT TGCAGTTACA GCGCAATAAT TTGGTGGACT ATTATGGCTT AAAGCCTGTC
      421 AGCCATTACA AAGATCTTGT AGTATATCGC CCCCTGCTTG ACATCAAGAA AAGCCAAATC
      481 TTGAACTATC TGAACACTAA TAAAATTAGC TACGCTATCG ATTCAACAAA TTCGGATACG
      541 AAATATCAAC GTAATAAAAT TCGTACCACA CTTAACGAAA ATAACTTCAC AAAAATTTTG
      601 GATCAAATTA ACAAAGCGAA CAATAATCTG AATAGCATCA AGAAAATTGT TGACAATTAC
      661 TTAAAGGATA ATATTATCAA TAACGAATTA AATTTAACAA AGGAATTGTT CTTGTTTGAC
      721 CAAAACTCAA TTCAGCGTAT TATCTATACT TACTTTAAGT TGATTAACAA GGAGTCTCTT
      781 TTATTAAATC GCTCGAACAA AACGATCATC GAAATCGTCA AACGTCTGGT TAACTCAAAT
      841 AAAAATCATT GGAAGATCAA CTTAAACGAT CATAGCCTTA TTAAAGACTA TAACAAGCTT
      901 TTCGTAATTA AAAACAGCTT ACTTGAGCCA AAGACGATTA TTATCAATGA TTTAAACGAT
      961 CTTATCAACC AAACGACGTT CAAAAATATT AAGGAAATTG AGCAAATTAT CTTGAAAGAC
     1021 AAAAACTTCT CGTATGTAAT CACAAATAAC TATGAAATGT ATAAGTCCAT CACGACAATC
     1081 GCCAACAAAA AGACGAATCG CTACTTTATT GACCGTAAGA TCAGCTACAA AACACGCTTG
     1141 TTGTCTCCAG TCGTATACAA CGTAAAGGAT AAGGTTATCT TGAACAAAAT TAAAAAGCAC
     1201 TACTGAAGAG CTTAGAGACC GGTACTAGTT CCTGCAGGGC TCGAGGTGTC AATCGTCGGA
     1261 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
     1321 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT
     1381 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA
     1441 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
     1501 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
     1561 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
     1621 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
     1681 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
     1741 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
     1801 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
     1861 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     1921 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     1981 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG
     2041 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA
     2101 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG
     2161 GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG
     2221 TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC
     2281 ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC
     2341 AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC
     2401 CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG
     2461 TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT
     2521 GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG
     2581 CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT
     2641 GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG
     2701 ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG
     2761 ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT
     2821 AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT
     2881 GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC
     2941 TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT
     3001 AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT
     3061 TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA
     3121 AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC GGCCAGTAGT CAAAAGCCTC
     3181 CGACCGGAGG CTTTTGACTT GGTCAGGTGG AGTGAATTCG CGGCCGCTTC TAGAGGCTCT
     3241 TCGTAATTAC GAAGAGGTCT CTA
//