LOCUS       Exported     2798 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2798
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      811..858
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2686..2702
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2703..2734
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0044"
                     /note=""
                     /translation="MKKEQVISRLKKLIDNNSLFSNIILNCKDEQTSWDVIYQIIYYAFNKNVKDLDFNKLKDQIQNNTHVDILTIGNNINITNQEVLDLINKMSLSATATQNIKFFIIKNAQNLKQSAANSLLKFLEEPPINTYGILLTNNYSEIINTIWSRCQLINIDNETKIDNKLNRFEELLISKNKDEILLFNKEMKAMNKNELVKMIDDAYNRTIIYQFTNLISCTLELLDDLKFLPLTNIAIDNYLIRIVEQI"
     primer_bind     complement(863..879)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(946..1534)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1705..2565)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2566..2670)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0044"
                     /note=""
                     /translation="MKKEQVISRLKKLIDNNSLFSNIILNCKDEQTSWDVIYQIIYYAFNKNVKDLDFNKLKDQIQNNTHVDILTIGNNINITNQEVLDLINKMSLSATATQNIKFFIIKNAQNLKQSAANSLLKFLEEPPINTYGILLTNNYSEIINTIWSRCQLINIDNETKIDNKLNRFEELLISKNKDEILLFNKEMKAMNKNELVKMIDDAYNRTIIYQFTNLISCTLELLDDLKFLPLTNIAIDNYLIRIVEQI"
ORIGIN
        1 ATGAAAAAGG AACAGGTCAT CTCGCGCCTG AAAAAGCTGA TTGACAATAA TTCCCTGTTT
       61 TCAAACATTA TCCTTAATTG TAAGGACGAG CAGACTAGTT GGGATGTTAT CTACCAGATT
      121 ATTTATTACG CTTTCAACAA GAATGTGAAG GACTTGGACT TTAATAAATT GAAGGATCAA
      181 ATCCAGAATA ATACACACGT TGACATCCTG ACAATCGGTA ATAACATTAA CATCACCAAT
      241 CAAGAAGTCT TGGACTTGAT CAACAAGATG TCTCTGAGTG CAACGGCTAC GCAGAATATT
      301 AAGTTCTTCA TCATTAAGAA CGCGCAGAAC CTTAAACAAA GTGCAGCTAA CAGCTTGTTA
      361 AAGTTTCTGG AGGAACCACC TATCAACACC TACGGCATCC TTTTGACAAA CAATTATTCC
      421 GAAATTATCA ACACGATCTG GAGCCGTTGC CAGTTAATCA ATATTGACAA TGAGACTAAG
      481 ATCGATAATA AATTGAATCG TTTCGAGGAG TTGCTTATCT CTAAGAATAA AGACGAAATC
      541 CTGTTATTCA ACAAGGAAAT GAAGGCCATG AACAAAAACG AGTTAGTGAA GATGATCGAC
      601 GATGCATATA ACCGTACTAT CATCTACCAA TTCACCAACT TGATTTCGTG TACTCTTGAA
      661 CTGTTGGATG ATCTTAAATT CTTGCCTCTT ACAAATATTG CTATCGACAA CTACCTGATC
      721 CGCATCGTGG AGCAGATTTG AAGAGCTTAG AGACCGGTAC TAGTTCCTGC AGGGCTCGAG
      781 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
      841 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA
      901 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG
      961 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
     1021 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
     1081 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
     1141 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     1201 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     1261 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     1321 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     1381 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     1441 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     1501 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG
     1561 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA
     1621 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT
     1681 ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC
     1741 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT
     1801 ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC
     1861 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC
     1921 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG
     1981 TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG
     2041 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG
     2101 ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG
     2161 TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT
     2221 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA
     2281 ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC
     2341 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC
     2401 AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC
     2461 TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC
     2521 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA
     2581 ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT
     2641 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC AGTCAGTAAA ACGACGGCCA
     2701 GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTCA GGTGGAGTGA ATTCGCGGCC
     2761 GCTTCTAGAG GCTCTTCGTA ATTACGAAGA GGTCTCTA
//