LOCUS       Exported     4064 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4064
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2077..2124
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3952..3968
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3969..4000
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0047"
                     /note="DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity."
                     /translation="MNTNKQSLYRTYRPKDFNSVAGHNNIKEILEKQIKDNRINHALLFSGQRGTGKTSVARIFAKTINCLNLTNSTACEQCNNCKLANQNQLIDIIEIDAASNNGVDEIREIKNSVSTLPLNSKYKVYIIDEVHMLTKQAFNALLKTLEEPPVYAIFILATTEFNKIPQTILSRCQIFNFTKIDKNSLKNRLQYIANQENYQIEKEVLDEMFYLSEGSLRDAINILEQLMLATDDLITIKTLKSIFLIATKQEQLQVVHQSLNNNTSFIISYFQKANDQGMNWDVFALGLIEILKEIIEYKLTNNTEFLNILEKNEVEQFNSVNANNLFILADNLAEAYFKTKAANISFNYLLLSLLKTINSNNNNLQTVSKTINTKQVEQNQEILKPNDITPEILDEPIIDEPVIQQPIIDEPVLQQPVIDEPVIQQPVIDNLSLTKDLDDKTLIKNTIENDKPLDSTNLDDPINEFDFYNQKEQTIDEICKTLSELKIKFNIHISQAINSKVKMLFNEDLISILIETKNYKNQIHNIEQLLEDLFLQDDNQLVNAQIASELFMLLDSKIISLTNDVIVLKTQTKAQANLINDSMLDNHVLQQIYNWFKKPYLIFAIDKMKWDEIKTIFIDLKNKNKLSEYSEINLKQLKEKYLTINDEIDQDLINKAKDLFNDDFMIGD"
     primer_bind     complement(2129..2145)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2212..2800)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2971..3831)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3832..3936)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0047"
                     /note="DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity."
                     /translation="MNTNKQSLYRTYRPKDFNSVAGHNNIKEILEKQIKDNRINHALLFSGQRGTGKTSVARIFAKTINCLNLTNSTACEQCNNCKLANQNQLIDIIEIDAASNNGVDEIREIKNSVSTLPLNSKYKVYIIDEVHMLTKQAFNALLKTLEEPPVYAIFILATTEFNKIPQTILSRCQIFNFTKIDKNSLKNRLQYIANQENYQIEKEVLDEMFYLSEGSLRDAINILEQLMLATDDLITIKTLKSIFLIATKQEQLQVVHQSLNNNTSFIISYFQKANDQGMNWDVFALGLIEILKEIIEYKLTNNTEFLNILEKNEVEQFNSVNANNLFILADNLAEAYFKTKAANISFNYLLLSLLKTINSNNNNLQTVSKTINTKQVEQNQEILKPNDITPEILDEPIIDEPVIQQPIIDEPVLQQPVIDEPVIQQPVIDNLSLTKDLDDKTLIKNTIENDKPLDSTNLDDPINEFDFYNQKEQTIDEICKTLSELKIKFNIHISQAINSKVKMLFNEDLISILIETKNYKNQIHNIEQLLEDLFLQDDNQLVNAQIASELFMLLDSKIISLTNDVIVLKTQTKAQANLINDSMLDNHVLQQIYNWFKKPYLIFAIDKMKWDEIKTIFIDLKNKNKLSEYSEINLKQLKEKYLTINDEIDQDLINKAKDLFNDDFMIGD"
ORIGIN
        1 ATGAACACTA ACAAGCAGTC CCTGTACCGT ACCTATCGCC CGAAAGACTT CAATAGTGTC
       61 GCCGGGCACA ACAATATTAA AGAGATCCTG GAAAAGCAAA TTAAGGACAA CCGCATTAAC
      121 CATGCATTAT TGTTTTCGGG TCAACGCGGT ACTGGTAAGA CTAGCGTAGC GCGTATCTTT
      181 GCCAAGACTA TCAACTGCTT AAACTTGACG AACTCCACTG CCTGCGAGCA ATGCAATAAC
      241 TGCAAATTAG CGAACCAGAA CCAGCTGATC GACATCATTG AGATCGATGC TGCATCGAAC
      301 AATGGCGTGG ATGAGATCCG CGAAATTAAA AACTCCGTAT CGACGCTTCC TTTAAACTCG
      361 AAGTATAAGG TATACATCAT TGACGAAGTA CACATGCTTA CTAAGCAAGC GTTTAACGCA
      421 CTTCTTAAGA CCTTGGAGGA GCCGCCTGTG TACGCAATTT TCATTTTGGC GACGACGGAA
      481 TTTAACAAAA TTCCGCAGAC AATTTTGTCG CGTTGCCAGA TTTTTAACTT TACAAAAATC
      541 GACAAAAATT CGCTTAAAAA CCGCTTACAG TATATCGCGA ATCAAGAGAA TTACCAAATT
      601 GAGAAGGAGG TGTTAGACGA AATGTTTTAT TTATCGGAGG GATCGCTTCG TGACGCAATC
      661 AATATCTTGG AACAACTTAT GTTAGCTACA GATGACCTGA TCACAATTAA GACGCTTAAA
      721 AGCATTTTCT TAATCGCCAC AAAACAAGAG CAACTGCAAG TAGTCCACCA GTCGTTGAAC
      781 AATAATACCA GTTTCATCAT TTCCTACTTT CAGAAAGCTA ACGACCAGGG CATGAATTGG
      841 GATGTGTTCG CCCTGGGCTT GATTGAAATC CTGAAAGAGA TTATTGAATA TAAATTAACC
      901 AACAATACAG AGTTTTTAAA CATCCTGGAA AAGAATGAGG TCGAACAGTT TAATTCAGTA
      961 AACGCGAATA ATCTTTTCAT CTTAGCGGAC AATCTGGCTG AGGCTTATTT CAAAACAAAG
     1021 GCGGCGAATA TCTCGTTTAA TTATCTGCTT CTGAGTTTGT TGAAAACCAT TAACAGTAAT
     1081 AATAATAACT TGCAGACTGT ATCCAAGACC ATTAATACAA AGCAAGTAGA ACAAAACCAG
     1141 GAGATTTTAA AACCTAACGA CATTACTCCC GAAATCTTGG ATGAACCTAT CATTGATGAG
     1201 CCAGTGATCC AACAGCCGAT CATCGACGAA CCCGTTTTGC AGCAACCTGT GATTGATGAA
     1261 CCGGTCATTC AACAGCCCGT TATTGACAAC CTGTCTTTAA CAAAGGACCT TGATGACAAA
     1321 ACTTTGATTA AGAATACTAT CGAAAATGAC AAACCCCTGG ATAGTACAAA TCTTGACGAC
     1381 CCCATTAACG AGTTTGATTT TTACAACCAA AAAGAGCAGA CCATTGATGA AATCTGCAAA
     1441 ACACTTAGTG AGCTGAAAAT CAAATTTAAT ATCCACATCA GCCAGGCCAT TAATTCAAAG
     1501 GTCAAAATGC TTTTTAACGA GGACCTTATT AGTATTCTTA TCGAAACCAA GAACTATAAG
     1561 AACCAAATCC ATAACATCGA ACAGTTGCTG GAGGACTTAT TTTTGCAAGA CGACAATCAA
     1621 CTTGTAAACG CGCAGATCGC GTCCGAATTA TTTATGTTAT TAGATTCTAA AATCATCTCT
     1681 CTGACCAACG ACGTGATTGT ATTAAAGACC CAGACGAAGG CCCAGGCAAA TTTGATTAAC
     1741 GATTCAATGT TAGACAACCA TGTACTGCAA CAGATCTACA ATTGGTTCAA GAAGCCCTAT
     1801 CTTATCTTTG CTATCGATAA AATGAAATGG GATGAGATCA AGACGATCTT CATCGACCTG
     1861 AAGAACAAAA ATAAGTTAAG TGAATACTCA GAAATTAATC TGAAGCAATT AAAAGAGAAG
     1921 TACTTAACGA TCAATGACGA AATTGATCAG GACCTTATCA ATAAAGCTAA GGATCTGTTT
     1981 AATGATGATT TTATGATCGG GGATTGAAGA GCTTAGAGAC CGGTACTAGT TCCTGCAGGG
     2041 CTCGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT
     2101 AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG
     2161 ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG
     2221 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
     2281 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
     2341 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
     2401 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
     2461 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
     2521 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
     2581 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
     2641 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
     2701 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
     2761 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC
     2821 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT
     2881 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA
     2941 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT
     3001 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC
     3061 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG
     3121 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG
     3181 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT
     3241 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT
     3301 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT
     3361 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT
     3421 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT
     3481 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT
     3541 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC
     3601 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA
     3661 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA
     3721 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA
     3781 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT
     3841 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
     3901 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA
     3961 CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTCAGGTG GAGTGAATTC
     4021 GCGGCCGCTT CTAGAGGCTC TTCGTAATTA CGAAGAGGTC TCTA
//