LOCUS       Exported     3326 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3326
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1339..1386
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3214..3230
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3231..3262
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0061"
                     /note="Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec)."
                     /translation="MLDINYIEQNLDEVIQRLNKRNQQDYSEDLKYAVEKNFKRKEILVKSEALKSRKNQLSKEIGILIKDNKNKEAEKAKAEVVSLNEQIIKLDDELRIVNEQILEKLSYIPNLPHKDIYFGKSDEDNVEIRKTKHNPLLTHSTPHWEIATKLGLVDFEKGVKLSGSRFLIYTGLGSKLVRAIADILLKRHEKHNYKEIFCPLIVNKSAMLGTGQLPKFSEDMYQVGEQYLIPTSEVPLTNLHANEILTYDMLPLKYTSFTQCFRQEAGSAGRDTKGMIRLHQFNKVELVKIVHPDQSMNELELLVKDAEDVLNMFDLPYRVVELCSGDIGFSSAKTYDLEVWFPEQNKYREISSCSNCTDFQARNIQTRFKDKDGKIKLVHTLNGSGVAVDRLIAAILENYWDGEKLVLPTILRPYFDKKEFLK"
     primer_bind     complement(1391..1407)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1474..2062)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2233..3093)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3094..3198)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0061"
                     /note="Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec)."
                     /translation="MLDINYIEQNLDEVIQRLNKRNQQDYSEDLKYAVEKNFKRKEILVKSEALKSRKNQLSKEIGILIKDNKNKEAEKAKAEVVSLNEQIIKLDDELRIVNEQILEKLSYIPNLPHKDIYFGKSDEDNVEIRKTKHNPLLTHSTPHWEIATKLGLVDFEKGVKLSGSRFLIYTGLGSKLVRAIADILLKRHEKHNYKEIFCPLIVNKSAMLGTGQLPKFSEDMYQVGEQYLIPTSEVPLTNLHANEILTYDMLPLKYTSFTQCFRQEAGSAGRDTKGMIRLHQFNKVELVKIVHPDQSMNELELLVKDAEDVLNMFDLPYRVVELCSGDIGFSSAKTYDLEVWFPEQNKYREISSCSNCTDFQARNIQTRFKDKDGKIKLVHTLNGSGVAVDRLIAAILENYWDGEKLVLPTILRPYFDKKEFLK"
ORIGIN
        1 ATGCTTGACA TCAACTATAT CGAGCAAAAC TTAGACGAGG TTATTCAGCG TCTTAATAAA
       61 CGCAACCAGC AAGATTACAG TGAGGACCTT AAATACGCGG TTGAGAAGAA TTTTAAACGT
      121 AAAGAAATTT TAGTAAAGTC TGAAGCGTTA AAATCACGCA AGAATCAATT GTCTAAAGAG
      181 ATCGGTATTC TTATCAAGGA CAACAAGAAT AAGGAAGCAG AAAAAGCAAA GGCTGAAGTC
      241 GTAAGCCTTA ACGAACAAAT TATCAAGCTT GATGACGAGT TGCGTATCGT TAATGAACAG
      301 ATTTTAGAGA AACTTAGCTA CATTCCAAAC CTTCCACACA AGGACATCTA TTTCGGTAAA
      361 AGCGACGAGG ACAATGTCGA AATCCGCAAA ACCAAACATA ATCCTTTGTT AACTCATTCG
      421 ACACCCCATT GGGAGATTGC GACCAAACTG GGCCTTGTGG ATTTTGAAAA AGGGGTCAAA
      481 TTGAGCGGCA GCCGCTTCCT GATCTATACA GGACTTGGCT CGAAACTGGT ACGTGCTATT
      541 GCCGATATCC TGTTAAAACG TCATGAGAAA CATAACTATA AAGAAATCTT CTGTCCGCTG
      601 ATTGTTAATA AAAGTGCCAT GCTTGGTACA GGCCAACTGC CGAAATTTTC AGAGGACATG
      661 TACCAAGTTG GCGAGCAGTA CCTTATCCCG ACGAGCGAGG TCCCCCTGAC TAACTTGCAT
      721 GCCAACGAGA TCCTTACTTA TGACATGCTG CCTTTGAAAT ATACGTCGTT CACGCAATGC
      781 TTCCGTCAGG AAGCAGGCAG CGCCGGTCGC GACACAAAGG GAATGATCCG CTTGCATCAG
      841 TTCAATAAAG TCGAGCTTGT GAAAATTGTC CATCCGGACC AGAGTATGAA TGAGCTGGAG
      901 TTACTTGTCA AGGATGCCGA GGATGTATTG AACATGTTTG ACTTGCCATA CCGCGTTGTC
      961 GAACTTTGCA GTGGCGACAT TGGATTTAGC TCCGCCAAGA CGTACGACTT AGAAGTTTGG
     1021 TTCCCAGAAC AAAACAAGTA TCGCGAAATT AGTTCATGTT CAAATTGTAC AGACTTCCAA
     1081 GCCCGCAACA TCCAAACACG TTTCAAAGAC AAAGATGGGA AGATCAAATT AGTTCATACC
     1141 TTGAATGGCA GCGGAGTTGC TGTGGACCGC CTTATTGCAG CTATCTTGGA AAATTATTGG
     1201 GATGGTGAAA AGTTGGTGTT ACCAACTATT CTGCGTCCCT ACTTTGACAA AAAAGAATTT
     1261 CTGAAGTGAA GAGCTTAGAG ACCGGTACTA GTTCCTGCAG GGCTCGAGGT GTCAATCGTC
     1321 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT
     1381 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA
     1441 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA
     1501 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     1561 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     1621 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     1681 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     1741 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     1801 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     1861 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     1921 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     1981 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     2041 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA
     2101 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC
     2161 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC
     2221 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT
     2281 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT
     2341 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT
     2401 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC
     2461 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA
     2521 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG
     2581 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT
     2641 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC
     2701 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT
     2761 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG
     2821 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC
     2881 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC
     2941 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT
     3001 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG
     3061 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG
     3121 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA
     3181 ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC
     3241 CTCCGACCGG AGGCTTTTGA CTTGGTCAGG TGGAGTGAAT TCGCGGCCGC TTCTAGAGGC
     3301 TCTTCGTAAT TACGAAGAGG TCTCTA
//