LOCUS       Exported     3032 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3032
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1045..1092
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     2920..2936
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2937..2968
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0063"
                     /note="Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines."
                     /translation="MKIRDIQIDGKVVQGPMAGVSNEAFRIISKQHGASLVYAEMVSVAGMVHDNKKTLNMLNVNEIEHPMSMQIFGNDVDEFIKATQWIEKNVDCDIIDLNLGCPAPKVAIRSQSGSALLKTPDLIYEIVKNVVKNTTKPVTAKIRLGWDKNSVNAVEVAKLIEKAGASAIAVHARTRNDFYTGHADWEKIKEVKQAVSIPVIGNGDVIDAKSAKKMLDETGCDAVMVSRACQGNPWIFDQINHYLKTGKELEKPSFEEWKTTVLQHLDLLVKLKTEQHAIKEFRKHLTWYLDVLNNKALTKILKEKANKIETIKDVEEIIKEYKEE"
     primer_bind     complement(1097..1113)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1180..1768)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1939..2799)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(2800..2904)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0063"
                     /note="Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines."
                     /translation="MKIRDIQIDGKVVQGPMAGVSNEAFRIISKQHGASLVYAEMVSVAGMVHDNKKTLNMLNVNEIEHPMSMQIFGNDVDEFIKATQWIEKNVDCDIIDLNLGCPAPKVAIRSQSGSALLKTPDLIYEIVKNVVKNTTKPVTAKIRLGWDKNSVNAVEVAKLIEKAGASAIAVHARTRNDFYTGHADWEKIKEVKQAVSIPVIGNGDVIDAKSAKKMLDETGCDAVMVSRACQGNPWIFDQINHYLKTGKELEKPSFEEWKTTVLQHLDLLVKLKTEQHAIKEFRKHLTWYLDVLNNKALTKILKEKANKIETIKDVEEIIKEYKEE"
ORIGIN
        1 ATGAAGATCC GTGATATTCA AATCGATGGT AAAGTCGTGC AAGGACCTAT GGCTGGTGTG
       61 TCCAATGAGG CATTTCGTAT TATTAGCAAG CAACATGGGG CTTCTCTTGT GTATGCCGAA
      121 ATGGTTTCAG TAGCGGGTAT GGTGCATGAT AACAAAAAGA CCTTGAATAT GCTGAATGTT
      181 AACGAAATTG AGCACCCCAT GTCGATGCAA ATTTTCGGAA ACGACGTTGA TGAGTTTATC
      241 AAAGCTACCC AATGGATTGA GAAGAACGTG GACTGTGATA TCATTGACCT TAATCTTGGC
      301 TGTCCGGCCC CAAAGGTTGC AATCCGCAGC CAATCAGGAT CGGCTTTACT CAAGACCCCT
      361 GATCTGATCT ACGAGATTGT CAAGAACGTG GTTAAAAATA CCACCAAACC AGTTACCGCG
      421 AAGATCCGCT TAGGATGGGA TAAAAACTCT GTCAACGCAG TCGAGGTCGC CAAGCTTATT
      481 GAGAAGGCCG GCGCATCGGC GATCGCGGTC CACGCTCGCA CACGCAACGA CTTCTATACT
      541 GGTCACGCAG ATTGGGAAAA GATTAAAGAA GTGAAGCAAG CTGTTTCCAT TCCGGTCATT
      601 GGGAATGGGG ATGTGATCGA CGCTAAAAGT GCTAAGAAAA TGCTTGATGA AACCGGGTGT
      661 GACGCGGTTA TGGTTTCTCG CGCATGCCAA GGGAACCCCT GGATCTTCGA CCAGATTAAT
      721 CATTACCTTA AGACTGGTAA GGAACTTGAA AAGCCTTCAT TTGAGGAGTG GAAAACAACG
      781 GTTTTACAGC ACTTGGATCT TTTAGTCAAA TTGAAAACAG AACAGCACGC GATTAAAGAA
      841 TTTCGCAAGC ATCTGACTTG GTACTTAGAC GTTTTAAACA ATAAAGCATT GACGAAAATT
      901 TTAAAAGAGA AGGCTAACAA GATTGAGACA ATCAAGGATG TCGAAGAAAT CATTAAGGAG
      961 TATAAAGAAG AGTGAAGAGC TTAGAGACCG GTACTAGTTC CTGCAGGGCT CGAGGTGTCA
     1021 ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG
     1081 AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT
     1141 AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC
     1201 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
     1261 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     1321 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
     1381 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     1441 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     1501 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     1561 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
     1621 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     1681 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     1741 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA
     1801 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT
     1861 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA
     1921 GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG
     1981 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA
     2041 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC
     2101 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC
     2161 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC
     2221 AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC
     2281 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC
     2341 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT
     2401 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC
     2461 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG
     2521 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG
     2581 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT
     2641 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC
     2701 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA
     2761 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA
     2821 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA
     2881 AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC
     2941 AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTCAGGTGGA GTGAATTCGC GGCCGCTTCT
     3001 AGAGGCTCTT CGTAATTACG AAGAGGTCTC TA
//