LOCUS       Exported     2900 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2900
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      913..960
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     2788..2804
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2805..2836
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             1..0
                     /label="MMSYN1_0066"
                     /note="Cof-like hydrolase"
                     /translation="MDKKNIIIFSDLDGTLLYDDYIFSPKTIEVVEKLYKKGIYLVPITARTIKDLKQKASLLQIDKFKGIIVASNGAQIYDYKTDKIIFDKTLPKEFIKEMFNRYHNKFFAKMIFYSPNCCYVFAEGKNSKYWAHQVMGLKYISVDSPDQIDEPITHFYIVTNSKATPEENLNEYKYLMNNYADSYKVDSYNNRVFDISVKGVDKGCGVAEVMKYLNLDEKTTHSYGFGDGPNDFSLLKACTTGIAMKNGIIELKEIADDITDYSNDKDGVARYICDKILNID"
     primer_bind     complement(965..981)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1048..1636)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1807..2667)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2668..2772)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0066"
                     /note="Cof-like hydrolase"
                     /translation="MDKKNIIIFSDLDGTLLYDDYIFSPKTIEVVEKLYKKGIYLVPITARTIKDLKQKASLLQIDKFKGIIVASNGAQIYDYKTDKIIFDKTLPKEFIKEMFNRYHNKFFAKMIFYSPNCCYVFAEGKNSKYWAHQVMGLKYISVDSPDQIDEPITHFYIVTNSKATPEENLNEYKYLMNNYADSYKVDSYNNRVFDISVKGVDKGCGVAEVMKYLNLDEKTTHSYGFGDGPNDFSLLKACTTGIAMKNGIIELKEIADDITDYSNDKDGVARYICDKILNID"
ORIGIN
        1 ATGGATAAAA AGAACATCAT TATCTTCTCT GATCTGGACG GGACGTTGCT TTACGACGAT
       61 TACATCTTCT CCCCCAAAAC TATCGAAGTA GTAGAGAAGT TGTATAAAAA AGGCATTTAT
      121 CTTGTGCCCA TCACCGCGCG TACAATTAAG GATTTAAAGC AGAAGGCTAG TTTACTTCAA
      181 ATTGATAAAT TCAAGGGAAT CATTGTTGCG TCGAATGGTG CACAGATCTA CGACTACAAG
      241 ACAGACAAAA TCATTTTTGA TAAAACATTG CCTAAAGAAT TCATTAAGGA GATGTTCAAT
      301 CGTTATCACA ACAAATTCTT CGCGAAAATG ATTTTCTATA GCCCCAACTG TTGCTATGTT
      361 TTCGCAGAAG GGAAGAATTC TAAGTATTGG GCTCATCAGG TGATGGGACT GAAATACATC
      421 TCAGTAGACT CTCCAGACCA AATTGACGAG CCTATTACAC ATTTTTATAT CGTCACTAAC
      481 TCCAAAGCAA CTCCGGAGGA AAACTTGAAC GAATATAAGT ACCTGATGAA TAATTATGCT
      541 GATTCATACA AGGTGGACAG TTATAACAAC CGTGTTTTCG ATATCTCGGT GAAAGGAGTG
      601 GATAAAGGTT GTGGTGTAGC CGAAGTAATG AAATATTTGA ATCTGGATGA AAAGACGACT
      661 CACAGCTATG GTTTCGGCGA CGGACCAAAC GACTTTTCGC TGTTGAAGGC GTGCACGACG
      721 GGCATTGCCA TGAAGAACGG GATTATTGAG TTGAAAGAGA TCGCGGATGA TATCACCGAT
      781 TATTCTAATG ACAAGGATGG GGTTGCTCGC TATATTTGCG ATAAAATCTT GAACATCGAT
      841 TGAAGAGCTT AGAGACCGGT ACTAGTTCCT GCAGGGCTCG AGGTGTCAAT CGTCGGAGCC
      901 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG
      961 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT
     1021 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     1081 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     1141 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     1201 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     1261 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     1321 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     1381 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     1441 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
     1501 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     1561 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     1621 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
     1681 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
     1741 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
     1801 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC
     1861 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
     1921 TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC
     1981 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC
     2041 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
     2101 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC
     2161 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
     2221 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT
     2281 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG
     2341 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
     2401 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA
     2461 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
     2521 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT
     2581 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG
     2641 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
     2701 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT
     2761 AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA
     2821 CCGGAGGCTT TTGACTTGGT CAGGTGGAGT GAATTCGCGG CCGCTTCTAG AGGCTCTTCG
     2881 TAATTACGAA GAGGTCTCTA
//