LOCUS       Exported     2897 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2897
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      910..957
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     2785..2801
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2802..2833
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0077"
                     /note=""
                     /translation="MLKNIKLIVTDLDGTVLHHGKLANDIDKPILEKAIKNNIHVTIATGQPYKSAKPRADLFNIGEHVDLAVLANGALISKISNFEPVYVNKIDNAIVNKMVKKLTELNICTVIFTATASDVYWNNIPFEVDSMIKRNWFERFNKTICSTDGNFDFIDPVQIMIFVPLEKNQILEDWFKAEKLDEHLTSMRNHIETIPIYEFTNITATKGKAIKKMAEILNVDINDVLVFGDNMNDMTMFEEIPNCVAVENAVDPIKQKAKYITDTNINGGVGKFIEKYILN"
     primer_bind     complement(962..978)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1045..1633)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(1804..2664)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2665..2769)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0077"
                     /note=""
                     /translation="MLKNIKLIVTDLDGTVLHHGKLANDIDKPILEKAIKNNIHVTIATGQPYKSAKPRADLFNIGEHVDLAVLANGALISKISNFEPVYVNKIDNAIVNKMVKKLTELNICTVIFTATASDVYWNNIPFEVDSMIKRNWFERFNKTICSTDGNFDFIDPVQIMIFVPLEKNQILEDWFKAEKLDEHLTSMRNHIETIPIYEFTNITATKGKAIKKMAEILNVDINDVLVFGDNMNDMTMFEEIPNCVAVENAVDPIKQKAKYITDTNINGGVGKFIEKYILN"
ORIGIN
        1 ATGTTGAAAA ATATCAAGTT GATTGTCACA GACCTTGACG GTACGGTTTT GCATCATGGT
       61 AAACTTGCGA ATGACATTGA CAAACCAATT TTGGAAAAGG CCATTAAGAA TAACATCCAT
      121 GTCACGATTG CAACCGGCCA ACCCTATAAA TCCGCTAAAC CTCGCGCCGA CCTTTTTAAT
      181 ATCGGTGAAC ACGTTGATCT TGCCGTGTTG GCCAACGGAG CTTTGATTTC CAAAATTAGT
      241 AACTTCGAGC CAGTGTACGT GAACAAAATC GATAACGCGA TTGTCAACAA GATGGTGAAG
      301 AAATTAACTG AGTTGAATAT TTGCACAGTA ATCTTTACGG CCACGGCTTC TGACGTTTAC
      361 TGGAATAACA TTCCTTTTGA AGTTGATTCA ATGATCAAAC GTAATTGGTT TGAGCGTTTC
      421 AACAAGACTA TTTGCTCGAC AGACGGAAAT TTTGACTTCA TCGACCCAGT TCAGATCATG
      481 ATTTTCGTGC CTCTTGAAAA GAATCAAATC TTAGAGGACT GGTTTAAAGC TGAGAAACTG
      541 GATGAACACT TAACATCGAT GCGTAACCAC ATCGAAACGA TCCCTATTTA CGAATTCACC
      601 AACATTACAG CGACGAAGGG GAAAGCCATT AAGAAGATGG CAGAGATCCT GAATGTTGAC
      661 ATTAACGATG TACTGGTTTT TGGTGACAAT ATGAATGATA TGACTATGTT CGAGGAAATT
      721 CCGAACTGCG TGGCCGTGGA GAATGCAGTT GATCCGATTA AACAAAAAGC TAAGTATATC
      781 ACTGACACTA ACATCAACGG TGGTGTAGGA AAATTCATCG AAAAATACAT CTTAAACTGA
      841 AGAGCTTAGA GACCGGTACT AGTTCCTGCA GGGCTCGAGG TGTCAATCGT CGGAGCCGCT
      901 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT
      961 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG
     1021 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
     1081 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
     1141 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     1201 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
     1261 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     1321 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     1381 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     1441 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
     1501 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     1561 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     1621 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA
     1681 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT
     1741 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA
     1801 CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC
     1861 CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG
     1921 CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT
     1981 AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT
     2041 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG
     2101 CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC
     2161 ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA
     2221 AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC
     2281 ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT
     2341 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG
     2401 TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT
     2461 GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG
     2521 ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC
     2581 CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC
     2641 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA
     2701 GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG
     2761 GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG
     2821 GAGGCTTTTG ACTTGGTCAG GTGGAGTGAA TTCGCGGCCG CTTCTAGAGG CTCTTCGTAA
     2881 TTACGAAGAG GTCTCTA
//