LOCUS       Exported     3014 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3014
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1027..1074
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2902..2918
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2919..2950
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0079"
                     /note="Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction."
                     /translation="MKILAIESSCDEFSISIIDNNKILTNVISSQIKDHQVFGGVVPELAARLHVQNFNWVLKAALTEANLSIKDIDYVAYTKSPGLIGSLIVGKLVAETISLYINKPILALDHIQGHIFGASIENEFIYPVLAMVVSGGHTQIEIINSANDFQIIGSTRDDAIGECYDKVARVLGLSYPGGPILDKLALKGNKDSYLLPVLKDEDNYDFSYSGLKTACINLIHNLNQKNQEINLEDFAASFQYTATNIVEKKLEKAIKEFKPKTLTVAGGVSANSEIRKIILRLGEKYSIKNTFVPKMSYCTDNAAMIAKLAYEKILLNNK"
     primer_bind     complement(1079..1095)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1162..1750)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1921..2781)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2782..2886)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0079"
                     /note="Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction."
                     /translation="MKILAIESSCDEFSISIIDNNKILTNVISSQIKDHQVFGGVVPELAARLHVQNFNWVLKAALTEANLSIKDIDYVAYTKSPGLIGSLIVGKLVAETISLYINKPILALDHIQGHIFGASIENEFIYPVLAMVVSGGHTQIEIINSANDFQIIGSTRDDAIGECYDKVARVLGLSYPGGPILDKLALKGNKDSYLLPVLKDEDNYDFSYSGLKTACINLIHNLNQKNQEINLEDFAASFQYTATNIVEKKLEKAIKEFKPKTLTVAGGVSANSEIRKIILRLGEKYSIKNTFVPKMSYCTDNAAMIAKLAYEKILLNNK"
ORIGIN
        1 ATGAAAATCC TGGCTATTGA AAGCAGCTGT GACGAGTTCT CTATCTCCAT CATTGACAAT
       61 AATAAGATCC TGACCAATGT GATTTCAAGT CAGATTAAGG ATCACCAGGT ATTCGGGGGC
      121 GTTGTTCCAG AGCTTGCCGC GCGTCTGCAT GTTCAGAATT TCAATTGGGT ATTGAAGGCT
      181 GCCCTGACTG AAGCCAACCT TTCAATTAAA GACATCGACT ACGTCGCCTA TACAAAAAGT
      241 CCAGGTTTAA TTGGTTCGTT AATTGTCGGA AAGTTAGTAG CTGAAACCAT CAGCTTATAC
      301 ATCAATAAGC CAATTTTGGC ATTAGACCAT ATCCAAGGTC ATATTTTCGG GGCCTCGATT
      361 GAGAATGAGT TTATCTACCC AGTCCTTGCC ATGGTTGTCT CTGGGGGGCA CACTCAAATC
      421 GAGATTATTA ACAGCGCAAA CGACTTCCAA ATTATTGGGA GCACTCGCGA CGATGCAATC
      481 GGGGAGTGTT ACGACAAAGT TGCACGCGTT CTTGGCTTAA GTTACCCCGG TGGACCTATT
      541 TTGGATAAAC TTGCGCTGAA GGGAAATAAG GACTCTTATC TGCTTCCAGT CTTGAAAGAC
      601 GAAGATAACT ATGACTTTTC CTATTCAGGG CTTAAAACAG CCTGCATCAA TTTGATTCAC
      661 AATTTGAATC AAAAGAACCA GGAAATCAAC CTGGAGGATT TCGCTGCGTC CTTCCAATAC
      721 ACGGCCACCA ATATTGTCGA GAAGAAGCTT GAGAAGGCGA TCAAGGAGTT CAAGCCAAAG
      781 ACATTGACGG TGGCAGGCGG GGTGTCAGCG AATTCCGAGA TTCGCAAAAT CATCTTGCGT
      841 TTAGGTGAAA AGTATTCAAT TAAGAATACG TTTGTTCCTA AAATGAGTTA CTGTACCGAC
      901 AATGCTGCTA TGATTGCAAA ACTTGCCTAT GAAAAAATCC TTTTAAACAA CAAGTGAAGA
      961 GCTTAGAGAC CGGTACTAGT TCCTGCAGGG CTCGAGGTGT CAATCGTCGG AGCCGCTGAG
     1021 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
     1081 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
     1141 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     1201 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     1261 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     1321 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     1381 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     1441 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     1501 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     1561 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     1621 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     1681 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     1741 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA
     1801 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
     1861 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG
     1921 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
     1981 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC
     2041 CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA
     2101 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
     2161 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
     2221 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT
     2281 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC
     2341 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT
     2401 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
     2461 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
     2521 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT
     2581 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC
     2641 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG
     2701 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
     2761 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
     2821 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT
     2881 TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG
     2941 GCTTTTGACT TGGTCAGGTG GAGTGAATTC GCGGCCGCTT CTAGAGGCTC TTCGTAATTA
     3001 CGAAGAGGTC TCTA
//