LOCUS Exported 2774 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2774 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 787..834 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" primer_bind 2662..2678 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2679..2710 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 1..0 /label="MMSYN1_0094" /note="" /translation="MQKRTIKSDTIFYSVILFLNLLTNFIYWITHAFNVVYVDEPTNLDIVLALDSASIAIWGLWISTFYAGICLYHSFIKKQLYQAYLLQLFIISMLISTGLIFIGISIINKTANINNWSALLRVVNVHFLLPTSMLLYLIFFRTNMIISKKSKLVGMWRILAVGLSYISWITYRTVPNVQVNLINKPFLYTSLQPSNIGWAIFMSLSFSSFILYFLTYLIIVLINNKINDKYGGCDAKTI" primer_bind complement(839..855) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(922..1510) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1681..2541) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2542..2646) /label="bla promoter" /note="ampicillin resistance gene promoter" CDS complement(1..0) /label="MMSYN1_0094" /note="" /translation="MQKRTIKSDTIFYSVILFLNLLTNFIYWITHAFNVVYVDEPTNLDIVLALDSASIAIWGLWISTFYAGICLYHSFIKKQLYQAYLLQLFIISMLISTGLIFIGISIINKTANINNWSALLRVVNVHFLLPTSMLLYLIFFRTNMIISKKSKLVGMWRILAVGLSYISWITYRTVPNVQVNLINKPFLYTSLQPSNIGWAIFMSLSFSSFILYFLTYLIIVLINNKINDKYGGCDAKTI" ORIGIN 1 ATGCAGAAAC GGACCATTAA ATCTGATACA ATTTTCTATT CAGTCATCCT TTTCCTGAAT 61 CTTCTGACGA ATTTTATATA CTGGATCACC CATGCCTTCA ACGTCGTTTA CGTGGACGAG 121 CCTACGAATC TGGATATAGT CTTAGCCCTT GACTCTGCGT CGATTGCGAT CTGGGGATTA 181 TGGATTTCCA CGTTTTACGC TGGCATATGT CTTTATCATT CATTCATAAA GAAGCAGCTG 241 TATCAGGCTT ACCTTTTGCA ACTGTTCATA ATCTCCATGT TAATTTCTAC CGGCCTGATT 301 TTTATTGGCA TTTCGATTAT TAATAAGACA GCGAACATCA ATAATTGGTC AGCTCTGCTG 361 CGCGTCGTAA ACGTTCACTT CTTGCTGCCC ACTAGTATGC TGCTGTACCT GATATTCTTC 421 AGAACAAATA TGATTATCTC TAAAAAATCT AAGCTGGTTG GGATGTGGCG CATCTTGGCT 481 GTCGGCCTGT CTTACATTTC TTGGATCACA TACCGGACCG TCCCTAACGT CCAAGTCAAC 541 TTGATTAACA AGCCCTTTTT ATACACCTCC TTACAACCCT CGAACATTGG CTGGGCAATT 601 TTTATGAGCT TGTCTTTTAG CAGTTTCATT TTGTACTTCT TAACCTACTT GATTATTGTC 661 CTTATCAATA ACAAGATCAA CGATAAGTAT GGAGGATGTG ATGCCAAAAC CATTTGAAGA 721 GCTTAGAGAC CGGTACTAGT TCCTGCAGGG CTCGAGGTGT CAATCGTCGG AGCCGCTGAG 781 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT 841 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG 901 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 961 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 1021 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 1081 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 1141 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 1201 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 1261 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 1321 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 1381 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 1441 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 1501 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 1561 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 1621 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG 1681 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT 1741 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC 1801 CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA 1861 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA 1921 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA 1981 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT 2041 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC 2101 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT 2161 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC 2221 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG 2281 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT 2341 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC 2401 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG 2461 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC 2521 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG 2581 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT 2641 TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG 2701 GCTTTTGACT TGGTCAGGTG GAGTGAATTC GCGGCCGCTT CTAGAGGCTC TTCGTAATTA 2761 CGAAGAGGTC TCTA //