LOCUS       Exported     3170 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3170
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1183..1230
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     3058..3074
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3075..3106
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0108"
                     /note="lipoprotein, putative"
                     /translation="MKKLLSIITGFSLLITPSLFAISCSSKVQVISKFDDITSIKNTGAFKNNQAFISRNELKEIVNSNNTTNSSTASSTAVMTSTSTTSTGTQPNNNDAKYASERLKALAANNFTKNKKQAWDSLQNTSMTFYKKVEPTAVNVLGYEQITKDNVEKLEKNLKTVFLVFKDNTKETEKLEVELLPEINNGNKVIDNGSLYLDLLEKPENLKLANQKSIIEVLRPEITKIKVVLQNTKNNNSTNKEDIKNTEVFNLLIKQLSIYLANTVKYFNSESGIITTNPTFSYKTRSNQIYDYIVKNKKDELYKKLETAFTSEFNKINFIDIFKDFQFDENNSNDNKKITTKIIKSSTNSSTSSSNSSTTTTTEPSSTTTR"
     primer_bind     complement(1235..1251)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1318..1906)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2077..2937)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2938..3042)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0108"
                     /note="lipoprotein, putative"
                     /translation="MKKLLSIITGFSLLITPSLFAISCSSKVQVISKFDDITSIKNTGAFKNNQAFISRNELKEIVNSNNTTNSSTASSTAVMTSTSTTSTGTQPNNNDAKYASERLKALAANNFTKNKKQAWDSLQNTSMTFYKKVEPTAVNVLGYEQITKDNVEKLEKNLKTVFLVFKDNTKETEKLEVELLPEINNGNKVIDNGSLYLDLLEKPENLKLANQKSIIEVLRPEITKIKVVLQNTKNNNSTNKEDIKNTEVFNLLIKQLSIYLANTVKYFNSESGIITTNPTFSYKTRSNQIYDYIVKNKKDELYKKLETAFTSEFNKINFIDIFKDFQFDENNSNDNKKITTKIIKSSTNSSTSSSNSSTTTTTEPSSTTTR"
ORIGIN
        1 ATGAAAAAAC TGCTGAGCAT TATTACCGGG TTCTCACTTC TTATTACCCC ATCATTATTC
       61 GCCATTTCCT GCTCCTCCAA AGTACAAGTT ATCAGTAAAT TCGACGATAT CACGTCGATC
      121 AAAAACACCG GTGCGTTCAA AAACAATCAG GCCTTTATCA GCCGTAACGA ACTGAAAGAA
      181 ATCGTTAATT CTAACAACAC CACCAACAGC AGCACCGCTT CTTCAACCGC TGTTATGACG
      241 AGTACCAGCA CCACCAGCAC AGGTACACAG CCGAATAACA ACGATGCTAA ATATGCGAGC
      301 GAGCGCCTGA AGGCGCTGGC CGCTAATAAT TTTACTAAAA ATAAGAAGCA GGCATGGGAT
      361 TCGCTTCAGA ACACATCAAT GACCTTCTAC AAGAAGGTTG AGCCAACCGC TGTGAACGTA
      421 TTGGGGTACG AGCAAATTAC TAAGGATAAC GTCGAGAAGT TGGAGAAGAA CCTCAAGACA
      481 GTCTTTTTAG TGTTTAAGGA TAACACCAAG GAAACCGAGA AGCTTGAAGT GGAATTATTG
      541 CCCGAAATCA ACAACGGCAA TAAGGTGATC GACAATGGTT CGTTATATTT GGACCTTCTT
      601 GAAAAACCAG AGAATTTAAA GCTTGCTAAT CAAAAATCTA TTATTGAAGT CCTGCGTCCG
      661 GAGATTACCA AAATCAAGGT CGTTTTGCAA AATACTAAAA ACAATAACTC AACAAACAAG
      721 GAAGATATTA AAAACACAGA AGTGTTTAAT TTGTTGATCA AGCAGTTGAG CATTTATCTT
      781 GCTAACACCG TGAAATATTT CAACAGTGAG AGCGGGATCA TTACAACAAA CCCCACCTTC
      841 AGTTACAAAA CACGCTCCAA CCAAATTTAT GATTACATCG TCAAGAATAA GAAGGATGAA
      901 TTATACAAGA AGCTTGAAAC CGCCTTCACC TCGGAATTTA ATAAAATTAA CTTTATTGAT
      961 ATTTTCAAGG ACTTTCAGTT TGACGAAAAC AATAGTAATG ATAACAAGAA GATCACAACT
     1021 AAGATCATCA AATCTTCGAC TAATTCAAGT ACTAGTAGTA GCAATTCATC CACAACTACA
     1081 ACCACCGAGC CATCGAGTAC TACCACCCGC TGAAGAGCTT AGAGACCGGT ACTAGTTCCT
     1141 GCAGGGCTCG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA ACCCCTTGGG
     1201 GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT GAGAGCTTGG
     1261 CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG CCATTAATTT
     1321 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
     1381 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
     1441 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
     1501 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
     1561 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
     1621 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
     1681 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
     1741 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
     1801 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
     1861 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
     1921 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT
     1981 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC
     2041 AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC
     2101 ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA
     2161 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA
     2221 ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG
     2281 CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC
     2341 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT
     2401 CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG
     2461 GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT
     2521 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA
     2581 TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA
     2641 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA
     2701 TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG
     2761 GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC
     2821 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG
     2881 AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT
     2941 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT
     3001 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA
     3061 AAACGACGGC CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT CAGGTGGAGT
     3121 GAATTCGCGG CCGCTTCTAG AGGCTCTTCG TAATTACGAA GAGGTCTCTA
//